Cargando…
Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)
The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160802/ https://www.ncbi.nlm.nih.gov/pubmed/30298074 http://dx.doi.org/10.3389/fpls.2018.01282 |
_version_ | 1783358847446941696 |
---|---|
author | Zhao, Junmin Zhai, Zhiwen Li, Yanan Geng, Shuaifeng Song, Gaoyuan Guan, Jiantao Jia, Meiling Wang, Fang Sun, Guoliang Feng, Nan Kong, Xingchen Chen, Liang Mao, Long Li, Aili |
author_facet | Zhao, Junmin Zhai, Zhiwen Li, Yanan Geng, Shuaifeng Song, Gaoyuan Guan, Jiantao Jia, Meiling Wang, Fang Sun, Guoliang Feng, Nan Kong, Xingchen Chen, Liang Mao, Long Li, Aili |
author_sort | Zhao, Junmin |
collection | PubMed |
description | The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this important crop has been conducted. Here, we performed a genome-wide survey in wheat and found 66 TCP genes that belonged to 22 homoeologous groups. We then mapped these genes on wheat chromosomes and found that several TCP genes were duplicated in wheat including the ortholog of the maize TEOSINTE BRANCHED 1. Expression study using both RT-PCR and in situ hybridization assay showed that most wheat TCP genes were expressed throughout development of young spike and immature seed. Cis-acting element survey along promoter regions suggests that subfunctionalization may have occurred for homoeologous genes. Moreover, protein–protein interaction experiments of three TCP proteins showed that they can form either homodimers or heterodimers. Finally, we characterized two TaTCP9 mutants from tetraploid wheat. Each of these two mutant lines contained a premature stop codon in the A subgenome homoeolog that was dominantly expressed over the B subgenome homoeolog. We observed that mutation caused increased spike and grain lengths. Together, our analysis of the wheat TCP gene family provides a start point for further functional study of these important transcription factors in wheat. |
format | Online Article Text |
id | pubmed-6160802 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61608022018-10-08 Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) Zhao, Junmin Zhai, Zhiwen Li, Yanan Geng, Shuaifeng Song, Gaoyuan Guan, Jiantao Jia, Meiling Wang, Fang Sun, Guoliang Feng, Nan Kong, Xingchen Chen, Liang Mao, Long Li, Aili Front Plant Sci Plant Science The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this important crop has been conducted. Here, we performed a genome-wide survey in wheat and found 66 TCP genes that belonged to 22 homoeologous groups. We then mapped these genes on wheat chromosomes and found that several TCP genes were duplicated in wheat including the ortholog of the maize TEOSINTE BRANCHED 1. Expression study using both RT-PCR and in situ hybridization assay showed that most wheat TCP genes were expressed throughout development of young spike and immature seed. Cis-acting element survey along promoter regions suggests that subfunctionalization may have occurred for homoeologous genes. Moreover, protein–protein interaction experiments of three TCP proteins showed that they can form either homodimers or heterodimers. Finally, we characterized two TaTCP9 mutants from tetraploid wheat. Each of these two mutant lines contained a premature stop codon in the A subgenome homoeolog that was dominantly expressed over the B subgenome homoeolog. We observed that mutation caused increased spike and grain lengths. Together, our analysis of the wheat TCP gene family provides a start point for further functional study of these important transcription factors in wheat. Frontiers Media S.A. 2018-09-10 /pmc/articles/PMC6160802/ /pubmed/30298074 http://dx.doi.org/10.3389/fpls.2018.01282 Text en Copyright © 2018 Zhao, Zhai, Li, Geng, Song, Guan, Jia, Wang, Sun, Feng, Kong, Chen, Mao and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhao, Junmin Zhai, Zhiwen Li, Yanan Geng, Shuaifeng Song, Gaoyuan Guan, Jiantao Jia, Meiling Wang, Fang Sun, Guoliang Feng, Nan Kong, Xingchen Chen, Liang Mao, Long Li, Aili Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) |
title | Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) |
title_full | Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) |
title_fullStr | Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) |
title_full_unstemmed | Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) |
title_short | Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) |
title_sort | genome-wide identification and expression profiling of the tcp family genes in spike and grain development of wheat (triticum aestivum l.) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160802/ https://www.ncbi.nlm.nih.gov/pubmed/30298074 http://dx.doi.org/10.3389/fpls.2018.01282 |
work_keys_str_mv | AT zhaojunmin genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT zhaizhiwen genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT liyanan genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT gengshuaifeng genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT songgaoyuan genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT guanjiantao genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT jiameiling genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT wangfang genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT sunguoliang genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT fengnan genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT kongxingchen genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT chenliang genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT maolong genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml AT liaili genomewideidentificationandexpressionprofilingofthetcpfamilygenesinspikeandgraindevelopmentofwheattriticumaestivuml |