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Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)

The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this...

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Autores principales: Zhao, Junmin, Zhai, Zhiwen, Li, Yanan, Geng, Shuaifeng, Song, Gaoyuan, Guan, Jiantao, Jia, Meiling, Wang, Fang, Sun, Guoliang, Feng, Nan, Kong, Xingchen, Chen, Liang, Mao, Long, Li, Aili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160802/
https://www.ncbi.nlm.nih.gov/pubmed/30298074
http://dx.doi.org/10.3389/fpls.2018.01282
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author Zhao, Junmin
Zhai, Zhiwen
Li, Yanan
Geng, Shuaifeng
Song, Gaoyuan
Guan, Jiantao
Jia, Meiling
Wang, Fang
Sun, Guoliang
Feng, Nan
Kong, Xingchen
Chen, Liang
Mao, Long
Li, Aili
author_facet Zhao, Junmin
Zhai, Zhiwen
Li, Yanan
Geng, Shuaifeng
Song, Gaoyuan
Guan, Jiantao
Jia, Meiling
Wang, Fang
Sun, Guoliang
Feng, Nan
Kong, Xingchen
Chen, Liang
Mao, Long
Li, Aili
author_sort Zhao, Junmin
collection PubMed
description The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this important crop has been conducted. Here, we performed a genome-wide survey in wheat and found 66 TCP genes that belonged to 22 homoeologous groups. We then mapped these genes on wheat chromosomes and found that several TCP genes were duplicated in wheat including the ortholog of the maize TEOSINTE BRANCHED 1. Expression study using both RT-PCR and in situ hybridization assay showed that most wheat TCP genes were expressed throughout development of young spike and immature seed. Cis-acting element survey along promoter regions suggests that subfunctionalization may have occurred for homoeologous genes. Moreover, protein–protein interaction experiments of three TCP proteins showed that they can form either homodimers or heterodimers. Finally, we characterized two TaTCP9 mutants from tetraploid wheat. Each of these two mutant lines contained a premature stop codon in the A subgenome homoeolog that was dominantly expressed over the B subgenome homoeolog. We observed that mutation caused increased spike and grain lengths. Together, our analysis of the wheat TCP gene family provides a start point for further functional study of these important transcription factors in wheat.
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spelling pubmed-61608022018-10-08 Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.) Zhao, Junmin Zhai, Zhiwen Li, Yanan Geng, Shuaifeng Song, Gaoyuan Guan, Jiantao Jia, Meiling Wang, Fang Sun, Guoliang Feng, Nan Kong, Xingchen Chen, Liang Mao, Long Li, Aili Front Plant Sci Plant Science The TCP family genes are plant-specific transcription factors and play important roles in plant development. TCPs have been evolutionarily and functionally studied in several plants. Although common wheat (Triticum aestivum L.) is a major staple crop worldwide, no systematic analysis of TCPs in this important crop has been conducted. Here, we performed a genome-wide survey in wheat and found 66 TCP genes that belonged to 22 homoeologous groups. We then mapped these genes on wheat chromosomes and found that several TCP genes were duplicated in wheat including the ortholog of the maize TEOSINTE BRANCHED 1. Expression study using both RT-PCR and in situ hybridization assay showed that most wheat TCP genes were expressed throughout development of young spike and immature seed. Cis-acting element survey along promoter regions suggests that subfunctionalization may have occurred for homoeologous genes. Moreover, protein–protein interaction experiments of three TCP proteins showed that they can form either homodimers or heterodimers. Finally, we characterized two TaTCP9 mutants from tetraploid wheat. Each of these two mutant lines contained a premature stop codon in the A subgenome homoeolog that was dominantly expressed over the B subgenome homoeolog. We observed that mutation caused increased spike and grain lengths. Together, our analysis of the wheat TCP gene family provides a start point for further functional study of these important transcription factors in wheat. Frontiers Media S.A. 2018-09-10 /pmc/articles/PMC6160802/ /pubmed/30298074 http://dx.doi.org/10.3389/fpls.2018.01282 Text en Copyright © 2018 Zhao, Zhai, Li, Geng, Song, Guan, Jia, Wang, Sun, Feng, Kong, Chen, Mao and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhao, Junmin
Zhai, Zhiwen
Li, Yanan
Geng, Shuaifeng
Song, Gaoyuan
Guan, Jiantao
Jia, Meiling
Wang, Fang
Sun, Guoliang
Feng, Nan
Kong, Xingchen
Chen, Liang
Mao, Long
Li, Aili
Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)
title Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)
title_full Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)
title_fullStr Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)
title_full_unstemmed Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)
title_short Genome-Wide Identification and Expression Profiling of the TCP Family Genes in Spike and Grain Development of Wheat (Triticum aestivum L.)
title_sort genome-wide identification and expression profiling of the tcp family genes in spike and grain development of wheat (triticum aestivum l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6160802/
https://www.ncbi.nlm.nih.gov/pubmed/30298074
http://dx.doi.org/10.3389/fpls.2018.01282
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