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Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling?
Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161023/ https://www.ncbi.nlm.nih.gov/pubmed/30041453 http://dx.doi.org/10.3390/vaccines6030044 |
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author | McCrudden, Maelíosa T. C. O’Donnell, Katherine Irwin, Chris R. Lundy, Fionnuala T. |
author_facet | McCrudden, Maelíosa T. C. O’Donnell, Katherine Irwin, Chris R. Lundy, Fionnuala T. |
author_sort | McCrudden, Maelíosa T. C. |
collection | PubMed |
description | Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal tissues, and gingival fibroblasts play an important role in maintaining and repairing the gingival tissues which are constantly exposed to external insults. In this study we examined the direct effects of LL-37 treatment on gingival fibroblasts and found that LL-37 significantly increased secretion of both interleukin 8 (IL-8) and IL-6 from these cells. LL-37 tended to decrease matrix metalloproteinase (MMP) activity in gingival fibroblasts, but this decrease did not reach statistical significance. LL-37 significantly increased tissue inhibitor of metalloproteinase-1 (TIMP-1) production by gingival fibroblasts, but had no significant effect on TIMP-2 levels. LL-37 was also shown to significantly increase production of basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and keratinocyte growth factor (KGF) in gingival fibroblasts. Taken together, these results suggest an important role for the host defence peptide, LL-37, in modulating the fibroblast response to remodeling in periodontal tissues. |
format | Online Article Text |
id | pubmed-6161023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61610232018-10-01 Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? McCrudden, Maelíosa T. C. O’Donnell, Katherine Irwin, Chris R. Lundy, Fionnuala T. Vaccines (Basel) Article Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal tissues, and gingival fibroblasts play an important role in maintaining and repairing the gingival tissues which are constantly exposed to external insults. In this study we examined the direct effects of LL-37 treatment on gingival fibroblasts and found that LL-37 significantly increased secretion of both interleukin 8 (IL-8) and IL-6 from these cells. LL-37 tended to decrease matrix metalloproteinase (MMP) activity in gingival fibroblasts, but this decrease did not reach statistical significance. LL-37 significantly increased tissue inhibitor of metalloproteinase-1 (TIMP-1) production by gingival fibroblasts, but had no significant effect on TIMP-2 levels. LL-37 was also shown to significantly increase production of basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and keratinocyte growth factor (KGF) in gingival fibroblasts. Taken together, these results suggest an important role for the host defence peptide, LL-37, in modulating the fibroblast response to remodeling in periodontal tissues. MDPI 2018-07-23 /pmc/articles/PMC6161023/ /pubmed/30041453 http://dx.doi.org/10.3390/vaccines6030044 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article McCrudden, Maelíosa T. C. O’Donnell, Katherine Irwin, Chris R. Lundy, Fionnuala T. Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
title | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
title_full | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
title_fullStr | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
title_full_unstemmed | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
title_short | Effects of LL-37 on Gingival Fibroblasts: A Role in Periodontal Tissue Remodeling? |
title_sort | effects of ll-37 on gingival fibroblasts: a role in periodontal tissue remodeling? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161023/ https://www.ncbi.nlm.nih.gov/pubmed/30041453 http://dx.doi.org/10.3390/vaccines6030044 |
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