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Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit

Plant ATP binding cassette (ABC) transporters are membrane proteins that are important for transporting a wide range of compounds, including secondary metabolites and phytohormones. In Arabidopsis, some members of the ABCB subfamily of ABC transporter, also known as Multi-Drug Resistance proteins (M...

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Autores principales: Ofori, Peter Amoako, Geisler, Markus, di Donato, Martin, Pengchao, Hao, Otagaki, Shungo, Matsumoto, Shogo, Shiratake, Katsuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161087/
https://www.ncbi.nlm.nih.gov/pubmed/30104476
http://dx.doi.org/10.3390/plants7030065
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author Ofori, Peter Amoako
Geisler, Markus
di Donato, Martin
Pengchao, Hao
Otagaki, Shungo
Matsumoto, Shogo
Shiratake, Katsuhiro
author_facet Ofori, Peter Amoako
Geisler, Markus
di Donato, Martin
Pengchao, Hao
Otagaki, Shungo
Matsumoto, Shogo
Shiratake, Katsuhiro
author_sort Ofori, Peter Amoako
collection PubMed
description Plant ATP binding cassette (ABC) transporters are membrane proteins that are important for transporting a wide range of compounds, including secondary metabolites and phytohormones. In Arabidopsis, some members of the ABCB subfamily of ABC transporter, also known as Multi-Drug Resistance proteins (MDRs), have been implicated in auxin transport. However, reports on the roles of the auxin-mediated ABCBs in fleshy fruit development are rare. Here, we present that SlABCB4, a member of the tomato ABCB subfamily, transports auxin in the developing fruit of tomato. Transient expression of SlABCB4-GFP fusion proteins in tobacco cells showed plasma membrane localization. The transport activity of SlABCB4, expressed in Nicotiana benthamiana protoplasts, revealed substrate specificity for indole-3-acetic acid export. Gene expression analysis of SlABCB4 revealed high expression levels at the early stages of fruit development. Therefore, SlABCB4 is considered to facilitate auxin distribution in tomato fruit, which is important for tomato fruit development.
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spelling pubmed-61610872018-10-01 Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit Ofori, Peter Amoako Geisler, Markus di Donato, Martin Pengchao, Hao Otagaki, Shungo Matsumoto, Shogo Shiratake, Katsuhiro Plants (Basel) Article Plant ATP binding cassette (ABC) transporters are membrane proteins that are important for transporting a wide range of compounds, including secondary metabolites and phytohormones. In Arabidopsis, some members of the ABCB subfamily of ABC transporter, also known as Multi-Drug Resistance proteins (MDRs), have been implicated in auxin transport. However, reports on the roles of the auxin-mediated ABCBs in fleshy fruit development are rare. Here, we present that SlABCB4, a member of the tomato ABCB subfamily, transports auxin in the developing fruit of tomato. Transient expression of SlABCB4-GFP fusion proteins in tobacco cells showed plasma membrane localization. The transport activity of SlABCB4, expressed in Nicotiana benthamiana protoplasts, revealed substrate specificity for indole-3-acetic acid export. Gene expression analysis of SlABCB4 revealed high expression levels at the early stages of fruit development. Therefore, SlABCB4 is considered to facilitate auxin distribution in tomato fruit, which is important for tomato fruit development. MDPI 2018-08-13 /pmc/articles/PMC6161087/ /pubmed/30104476 http://dx.doi.org/10.3390/plants7030065 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ofori, Peter Amoako
Geisler, Markus
di Donato, Martin
Pengchao, Hao
Otagaki, Shungo
Matsumoto, Shogo
Shiratake, Katsuhiro
Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit
title Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit
title_full Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit
title_fullStr Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit
title_full_unstemmed Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit
title_short Tomato ATP-Binding Cassette Transporter SlABCB4 Is Involved in Auxin Transport in the Developing Fruit
title_sort tomato atp-binding cassette transporter slabcb4 is involved in auxin transport in the developing fruit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161087/
https://www.ncbi.nlm.nih.gov/pubmed/30104476
http://dx.doi.org/10.3390/plants7030065
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