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Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?

Background: Dynamic light scattering (DLS) is an important tool to characterize colloidal systems and adequate sizing is particularly critical in the field of protein formulations. Among the different factors that can influence the measurement result, the effect of laser power has so far not been st...

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Detalles Bibliográficos
Autores principales: Aleandri, Simone, Vaccaro, Andrea, Armenta, Ricardo, Völker, Andreas Charles, Kuentz, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161136/
https://www.ncbi.nlm.nih.gov/pubmed/30018274
http://dx.doi.org/10.3390/pharmaceutics10030094
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author Aleandri, Simone
Vaccaro, Andrea
Armenta, Ricardo
Völker, Andreas Charles
Kuentz, Martin
author_facet Aleandri, Simone
Vaccaro, Andrea
Armenta, Ricardo
Völker, Andreas Charles
Kuentz, Martin
author_sort Aleandri, Simone
collection PubMed
description Background: Dynamic light scattering (DLS) is an important tool to characterize colloidal systems and adequate sizing is particularly critical in the field of protein formulations. Among the different factors that can influence the measurement result, the effect of laser power has so far not been studied thoroughly. Methods: The sensitivity of a DLS instrument was first considered on a theoretical level, followed by experiments using DLS instruments, equipped with two different lasers of (nominal) 45 mW, and 100 mW, respectively. This work analyzes dilute colloidal dispersions of lysozyme as model protein. Results: Theoretical findings agreed with experiments in that only enhanced laser power of 100 mW laser allowed measuring a 0.1 mg/mL protein dispersion in a reliable manner. Results confirmed the usefulness of the presented theoretical considerations in improving a general understanding of the limiting factors in DLS. Conclusions: Laser power is a critical aspect regarding adequate colloidal analysis by DLS. Practical guidance is provided to help scientists specifically with measuring dilute samples to choose both an optimal instrument configuration as well as a robust experimental procedure.
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spelling pubmed-61611362018-10-01 Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power? Aleandri, Simone Vaccaro, Andrea Armenta, Ricardo Völker, Andreas Charles Kuentz, Martin Pharmaceutics Article Background: Dynamic light scattering (DLS) is an important tool to characterize colloidal systems and adequate sizing is particularly critical in the field of protein formulations. Among the different factors that can influence the measurement result, the effect of laser power has so far not been studied thoroughly. Methods: The sensitivity of a DLS instrument was first considered on a theoretical level, followed by experiments using DLS instruments, equipped with two different lasers of (nominal) 45 mW, and 100 mW, respectively. This work analyzes dilute colloidal dispersions of lysozyme as model protein. Results: Theoretical findings agreed with experiments in that only enhanced laser power of 100 mW laser allowed measuring a 0.1 mg/mL protein dispersion in a reliable manner. Results confirmed the usefulness of the presented theoretical considerations in improving a general understanding of the limiting factors in DLS. Conclusions: Laser power is a critical aspect regarding adequate colloidal analysis by DLS. Practical guidance is provided to help scientists specifically with measuring dilute samples to choose both an optimal instrument configuration as well as a robust experimental procedure. MDPI 2018-07-17 /pmc/articles/PMC6161136/ /pubmed/30018274 http://dx.doi.org/10.3390/pharmaceutics10030094 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aleandri, Simone
Vaccaro, Andrea
Armenta, Ricardo
Völker, Andreas Charles
Kuentz, Martin
Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?
title Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?
title_full Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?
title_fullStr Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?
title_full_unstemmed Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?
title_short Dynamic Light Scattering of Biopharmaceutics—Can Analytical Performance Be Enhanced by Laser Power?
title_sort dynamic light scattering of biopharmaceutics—can analytical performance be enhanced by laser power?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161136/
https://www.ncbi.nlm.nih.gov/pubmed/30018274
http://dx.doi.org/10.3390/pharmaceutics10030094
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