Cargando…
Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques
Pulmonary delivery of drugs and vaccines is an established route of administration, with particulate-based carriers becoming an attractive strategy to enhance the benefits of pulmonary therapeutic delivery. Despite the increasing number of publications using the pulmonary route of delivery, the lack...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161314/ https://www.ncbi.nlm.nih.gov/pubmed/29996506 http://dx.doi.org/10.3390/vaccines6030041 |
_version_ | 1783358961500553216 |
---|---|
author | Kunda, Nitesh K. Price, Dominique N. Muttil, Pavan |
author_facet | Kunda, Nitesh K. Price, Dominique N. Muttil, Pavan |
author_sort | Kunda, Nitesh K. |
collection | PubMed |
description | Pulmonary delivery of drugs and vaccines is an established route of administration, with particulate-based carriers becoming an attractive strategy to enhance the benefits of pulmonary therapeutic delivery. Despite the increasing number of publications using the pulmonary route of delivery, the lack of effective and uniform administration techniques in preclinical models generally results in poor translational success. In this study, we used the IVIS Spectrum small-animal in vivo imaging system to compare the respiratory tract deposition and distribution pattern of a microsphere suspension (5 µm) in mice after 1, 4, and 24 h when delivered by oropharyngeal aspiration, the Microsprayer(®) Aerosolizer, and the BioLite Intubation System, three-widely reported preclinical inhalation techniques. We saw no significant differences in microsphere deposition in whole body images and excised lungs (at 1, 4, and 24 h); however, the three-dimensional (3D) images showed more localized deposition in the lungs with the MicroSprayer(®) and BioLite delivery techniques. Further, oropharyngeal aspiration (at 1 h) showed microsphere deposition in the oral cavity, in contrast to the MicroSprayer(®) and BioLite systems. The studies shown here will allow researchers to choose the appropriate pulmonary delivery method in animal models based on their study requirements. |
format | Online Article Text |
id | pubmed-6161314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61613142018-10-01 Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques Kunda, Nitesh K. Price, Dominique N. Muttil, Pavan Vaccines (Basel) Communication Pulmonary delivery of drugs and vaccines is an established route of administration, with particulate-based carriers becoming an attractive strategy to enhance the benefits of pulmonary therapeutic delivery. Despite the increasing number of publications using the pulmonary route of delivery, the lack of effective and uniform administration techniques in preclinical models generally results in poor translational success. In this study, we used the IVIS Spectrum small-animal in vivo imaging system to compare the respiratory tract deposition and distribution pattern of a microsphere suspension (5 µm) in mice after 1, 4, and 24 h when delivered by oropharyngeal aspiration, the Microsprayer(®) Aerosolizer, and the BioLite Intubation System, three-widely reported preclinical inhalation techniques. We saw no significant differences in microsphere deposition in whole body images and excised lungs (at 1, 4, and 24 h); however, the three-dimensional (3D) images showed more localized deposition in the lungs with the MicroSprayer(®) and BioLite delivery techniques. Further, oropharyngeal aspiration (at 1 h) showed microsphere deposition in the oral cavity, in contrast to the MicroSprayer(®) and BioLite systems. The studies shown here will allow researchers to choose the appropriate pulmonary delivery method in animal models based on their study requirements. MDPI 2018-07-10 /pmc/articles/PMC6161314/ /pubmed/29996506 http://dx.doi.org/10.3390/vaccines6030041 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Kunda, Nitesh K. Price, Dominique N. Muttil, Pavan Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques |
title | Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques |
title_full | Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques |
title_fullStr | Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques |
title_full_unstemmed | Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques |
title_short | Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques |
title_sort | respiratory tract deposition and distribution pattern of microparticles in mice using different pulmonary delivery techniques |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161314/ https://www.ncbi.nlm.nih.gov/pubmed/29996506 http://dx.doi.org/10.3390/vaccines6030041 |
work_keys_str_mv | AT kundaniteshk respiratorytractdepositionanddistributionpatternofmicroparticlesinmiceusingdifferentpulmonarydeliverytechniques AT pricedominiquen respiratorytractdepositionanddistributionpatternofmicroparticlesinmiceusingdifferentpulmonarydeliverytechniques AT muttilpavan respiratorytractdepositionanddistributionpatternofmicroparticlesinmiceusingdifferentpulmonarydeliverytechniques |