Cargando…
Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle
BACKGROUND: Members of the species Kocuria rhizophila, belonging to the family Micrococcaceae in the phylum Actinobacteria, have been isolated from a wide variety of natural sources, such as soil, freshwater, fish gut, and clinical specimens. K. rhizophila is important from an industrial viewpoint,...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161389/ https://www.ncbi.nlm.nih.gov/pubmed/30275909 http://dx.doi.org/10.1186/s13099-018-0270-9 |
_version_ | 1783358977438908416 |
---|---|
author | Whon, Tae Woong Kim, Hyun Sik Bae, Jin-Woo |
author_facet | Whon, Tae Woong Kim, Hyun Sik Bae, Jin-Woo |
author_sort | Whon, Tae Woong |
collection | PubMed |
description | BACKGROUND: Members of the species Kocuria rhizophila, belonging to the family Micrococcaceae in the phylum Actinobacteria, have been isolated from a wide variety of natural sources, such as soil, freshwater, fish gut, and clinical specimens. K. rhizophila is important from an industrial viewpoint, because the bacterium grows rapidly with high cell density and exhibits robustness at various growth conditions. However, the bacterium is an opportunistic pathogen involved in human infections. Here, we sequenced and analyzed the genome of the K. rhizophila strain BT304, isolated from the small intestine of adult castrated beef cattle. RESULTS: The genome of K. rhizophila BT304 consisted of a single circular chromosome of 2,763,150 bp with a GC content of 71.2%. The genome contained 2359 coding sequences, 51 tRNA genes, and 9 rRNA genes. Sequence annotations with the RAST server revealed many genes related to amino acid, carbohydrate, and protein metabolism. Moreover, the genome contained genes related to branched chain amino acid biosynthesis and degradation. Analysis of the OrthoANI values revealed that the genome has high similarity (> 97.8%) with other K. rhizophila strains, such as DC2201, FDAARGOS 302, and G2. Comparative genomic analysis further revealed that the antibiotic properties of K. rhizophila vary among the strains. CONCLUSION: The relatively small number of virulence-related genes and the great potential in production of host available nutrients suggest potential application of the BT304 strain as a probiotic in breeding beef cattle. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0270-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6161389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-61613892018-10-01 Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle Whon, Tae Woong Kim, Hyun Sik Bae, Jin-Woo Gut Pathog Genome Report BACKGROUND: Members of the species Kocuria rhizophila, belonging to the family Micrococcaceae in the phylum Actinobacteria, have been isolated from a wide variety of natural sources, such as soil, freshwater, fish gut, and clinical specimens. K. rhizophila is important from an industrial viewpoint, because the bacterium grows rapidly with high cell density and exhibits robustness at various growth conditions. However, the bacterium is an opportunistic pathogen involved in human infections. Here, we sequenced and analyzed the genome of the K. rhizophila strain BT304, isolated from the small intestine of adult castrated beef cattle. RESULTS: The genome of K. rhizophila BT304 consisted of a single circular chromosome of 2,763,150 bp with a GC content of 71.2%. The genome contained 2359 coding sequences, 51 tRNA genes, and 9 rRNA genes. Sequence annotations with the RAST server revealed many genes related to amino acid, carbohydrate, and protein metabolism. Moreover, the genome contained genes related to branched chain amino acid biosynthesis and degradation. Analysis of the OrthoANI values revealed that the genome has high similarity (> 97.8%) with other K. rhizophila strains, such as DC2201, FDAARGOS 302, and G2. Comparative genomic analysis further revealed that the antibiotic properties of K. rhizophila vary among the strains. CONCLUSION: The relatively small number of virulence-related genes and the great potential in production of host available nutrients suggest potential application of the BT304 strain as a probiotic in breeding beef cattle. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0270-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-27 /pmc/articles/PMC6161389/ /pubmed/30275909 http://dx.doi.org/10.1186/s13099-018-0270-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Genome Report Whon, Tae Woong Kim, Hyun Sik Bae, Jin-Woo Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle |
title | Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle |
title_full | Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle |
title_fullStr | Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle |
title_full_unstemmed | Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle |
title_short | Complete genome sequence of Kocuria rhizophila BT304, isolated from the small intestine of castrated beef cattle |
title_sort | complete genome sequence of kocuria rhizophila bt304, isolated from the small intestine of castrated beef cattle |
topic | Genome Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161389/ https://www.ncbi.nlm.nih.gov/pubmed/30275909 http://dx.doi.org/10.1186/s13099-018-0270-9 |
work_keys_str_mv | AT whontaewoong completegenomesequenceofkocuriarhizophilabt304isolatedfromthesmallintestineofcastratedbeefcattle AT kimhyunsik completegenomesequenceofkocuriarhizophilabt304isolatedfromthesmallintestineofcastratedbeefcattle AT baejinwoo completegenomesequenceofkocuriarhizophilabt304isolatedfromthesmallintestineofcastratedbeefcattle |