Effect of root canal preparation, obturation, and retreatment on the induction of dentinal microcracks: A microcomputed tomography study

INTRODUCTION: This study evaluated the effect of root canal preparation, obturation, and different techniques of retreatment on the percentage increase in the number of dentinal microcracks using microcomputed tomography (CT) analysis. MATERIALS AND METHODS: Forty-five mandibular premolars after micro-CT scanning were prepared using Protaper universal files up to F4 and obturated. After the second micro-CT scanning again, the samples were randomly divided into three groups (n = 15) for retreatment procedure. In Group 1, retreatment was done with Protaper universal D1, D2, and D3 retreatment files followed by Protaper universal file till F4. In Group 2, retreatment was performed with R-Endo reciprocating motion, Re, R1, R2, R3, and Rs followed by Hero Shaper size #40. In Group 3, retreatment was performed with H-files and xylene till size #40. After gutta percha removal, the samples were again scanned for micro-CT for the evaluation of percentage increase in dentinal microcracks. Data were analyzed using “Kruskal–Wallis test ANOVA” and “student t-test” at 5% level of significance. RESULTS: Few unprepared samples showed preexisting microcracks. There were statistically significant percentage increase dentinal cracks after root canal preparation and obturation as well as after retreatment procedure with statistically significant percentage increase in the number of dentinal microcracks between all three groups. CONCLUSION: Intraradicular procedures such as shaping and cleaning and obturation as well as the use of retreatment file lead to the induction and propagation of dentinal microcracks. In the present study, the maximum microcracks were produced with Protaper universal retreatment file system followed by R-Endo system. H-files produced minimum cracks during retreatment procedures.