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Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls

Colorectal cancer (CRC), a primary cause of morbidity and mortality worldwide is expected to rise in the coming years. A better understanding of the metabolic changes taking place during the disease progression is needed for effective improvements of screening strategies and treatments. In the prese...

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Autores principales: Le Gall, Gwénaëlle, Guttula, Kiran, Kellingray, Lee, Tett, Adrian J., ten Hoopen, Rogier, Kemsley, Kate E., Savva, George M., Ibrahim, Ashraf, Narbad, Arjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161785/
https://www.ncbi.nlm.nih.gov/pubmed/30279959
http://dx.doi.org/10.18632/oncotarget.26022
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author Le Gall, Gwénaëlle
Guttula, Kiran
Kellingray, Lee
Tett, Adrian J.
ten Hoopen, Rogier
Kemsley, Kate E.
Savva, George M.
Ibrahim, Ashraf
Narbad, Arjan
author_facet Le Gall, Gwénaëlle
Guttula, Kiran
Kellingray, Lee
Tett, Adrian J.
ten Hoopen, Rogier
Kemsley, Kate E.
Savva, George M.
Ibrahim, Ashraf
Narbad, Arjan
author_sort Le Gall, Gwénaëlle
collection PubMed
description Colorectal cancer (CRC), a primary cause of morbidity and mortality worldwide is expected to rise in the coming years. A better understanding of the metabolic changes taking place during the disease progression is needed for effective improvements of screening strategies and treatments. In the present study, Nuclear Magnetic Resonance (NMR) metabolomics was used to quantify the absolute concentrations of metabolites in faecal extracts from two cohorts of CRC patients and healthy controls. The quantification of over 80 compounds revealed that patients with CRC had increased faecal concentrations of branched chain fatty acids (BCFA), isovalerate and isobutyrate plus valerate and phenylacetate but diminished concentrations of amino acids, sugars, methanol and bile acids (deoxycholate, lithodeoxycholate and cholate). These results suggest that alterations in microbial activity and composition could have triggered an increase in utilisation of host intestinal slough cells and mucins and led to an increase in BCFA, valerate and phenylacetate. Concurrently, a general reduction in the microbial metabolic function may have led to reduced levels of other components (amino acids, sugars and bile acids) normally produced under healthy conditions. This study provides a thorough listing of the most abundant compounds found in human faecal waters and presents a template for absolute quantification of metabolites. The production of BCFA and phenylacetate in colonic carcinogenesis warrants further investigations.
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spelling pubmed-61617852018-10-02 Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls Le Gall, Gwénaëlle Guttula, Kiran Kellingray, Lee Tett, Adrian J. ten Hoopen, Rogier Kemsley, Kate E. Savva, George M. Ibrahim, Ashraf Narbad, Arjan Oncotarget Research Paper Colorectal cancer (CRC), a primary cause of morbidity and mortality worldwide is expected to rise in the coming years. A better understanding of the metabolic changes taking place during the disease progression is needed for effective improvements of screening strategies and treatments. In the present study, Nuclear Magnetic Resonance (NMR) metabolomics was used to quantify the absolute concentrations of metabolites in faecal extracts from two cohorts of CRC patients and healthy controls. The quantification of over 80 compounds revealed that patients with CRC had increased faecal concentrations of branched chain fatty acids (BCFA), isovalerate and isobutyrate plus valerate and phenylacetate but diminished concentrations of amino acids, sugars, methanol and bile acids (deoxycholate, lithodeoxycholate and cholate). These results suggest that alterations in microbial activity and composition could have triggered an increase in utilisation of host intestinal slough cells and mucins and led to an increase in BCFA, valerate and phenylacetate. Concurrently, a general reduction in the microbial metabolic function may have led to reduced levels of other components (amino acids, sugars and bile acids) normally produced under healthy conditions. This study provides a thorough listing of the most abundant compounds found in human faecal waters and presents a template for absolute quantification of metabolites. The production of BCFA and phenylacetate in colonic carcinogenesis warrants further investigations. Impact Journals LLC 2018-09-07 /pmc/articles/PMC6161785/ /pubmed/30279959 http://dx.doi.org/10.18632/oncotarget.26022 Text en Copyright: © 2018 Le Gall et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Le Gall, Gwénaëlle
Guttula, Kiran
Kellingray, Lee
Tett, Adrian J.
ten Hoopen, Rogier
Kemsley, Kate E.
Savva, George M.
Ibrahim, Ashraf
Narbad, Arjan
Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls
title Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls
title_full Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls
title_fullStr Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls
title_full_unstemmed Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls
title_short Metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls
title_sort metabolite quantification of faecal extracts from colorectal cancer patients and healthy controls
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161785/
https://www.ncbi.nlm.nih.gov/pubmed/30279959
http://dx.doi.org/10.18632/oncotarget.26022
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