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Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype
Cell adhesion plays a key role in cell behavior, in terms of migration, proliferation, differentiation and apoptosis. All of these events concur with tissue regeneration and remodeling mechanisms, integrating a complex network of intracellular signaling modules. Morphogenetic responses, which involv...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161806/ https://www.ncbi.nlm.nih.gov/pubmed/24756781 http://dx.doi.org/10.5301/jabfm.5000186 |
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author | Boccafoschi, Francesca Rasponi, Marco Ramella, Martina Ferreira, Ana Marina Vesentini, Simone Cannas, Mario |
author_facet | Boccafoschi, Francesca Rasponi, Marco Ramella, Martina Ferreira, Ana Marina Vesentini, Simone Cannas, Mario |
author_sort | Boccafoschi, Francesca |
collection | PubMed |
description | Cell adhesion plays a key role in cell behavior, in terms of migration, proliferation, differentiation and apoptosis. All of these events concur with tissue regeneration and remodeling mechanisms, integrating a complex network of intracellular signaling modules. Morphogenetic responses, which involve changes in cell shape, proliferation and differentiation, are thought to be controlled by both biochemical and biophysical cues. Indeed, the extracellular matrix not only displays adhesive ligands necessary for cell adhesion but also plays an essential biomechanical role — responsible, for instance, for the acquisition of the contractile phenotype. The substrate topography around the forming tissues and the associated mechanical stresses that are generated regulate cellular morphology, proliferation and differentiation. Thus, the ability to tailor topographical features around cells can be a crucial design parameter in tissue engineering applications, inducing cells to exhibit the required performances. In this work, we designed micropillared substrates using highly spaced arrays (interspacing equal to 25 μm) to evaluate the effects of topography on C2C12 myoblasts' adhesion and differentiation. Optical and fluorescence microscopy images were used to observe cell adhesion, together with Western blot analysis on vinculin and focal adhesion kinase (FAK) expression, a protein highly involved in adhesive processes. Differentiation marker (Myf5, myogenin and myosin heavy chain [MHC]) expression was also studied, in relation to the effect of different substrate topographies on the enhancement of a contractile phenotype. Our results demonstrated that microstructured surfaces may play a key role in the regeneration of functional tissues. |
format | Online Article Text |
id | pubmed-6161806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-61618062018-10-11 Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype Boccafoschi, Francesca Rasponi, Marco Ramella, Martina Ferreira, Ana Marina Vesentini, Simone Cannas, Mario J Appl Biomater Funct Mater Original Article Cell adhesion plays a key role in cell behavior, in terms of migration, proliferation, differentiation and apoptosis. All of these events concur with tissue regeneration and remodeling mechanisms, integrating a complex network of intracellular signaling modules. Morphogenetic responses, which involve changes in cell shape, proliferation and differentiation, are thought to be controlled by both biochemical and biophysical cues. Indeed, the extracellular matrix not only displays adhesive ligands necessary for cell adhesion but also plays an essential biomechanical role — responsible, for instance, for the acquisition of the contractile phenotype. The substrate topography around the forming tissues and the associated mechanical stresses that are generated regulate cellular morphology, proliferation and differentiation. Thus, the ability to tailor topographical features around cells can be a crucial design parameter in tissue engineering applications, inducing cells to exhibit the required performances. In this work, we designed micropillared substrates using highly spaced arrays (interspacing equal to 25 μm) to evaluate the effects of topography on C2C12 myoblasts' adhesion and differentiation. Optical and fluorescence microscopy images were used to observe cell adhesion, together with Western blot analysis on vinculin and focal adhesion kinase (FAK) expression, a protein highly involved in adhesive processes. Differentiation marker (Myf5, myogenin and myosin heavy chain [MHC]) expression was also studied, in relation to the effect of different substrate topographies on the enhancement of a contractile phenotype. Our results demonstrated that microstructured surfaces may play a key role in the regeneration of functional tissues. SAGE Publications 2014-04-18 2015-07 /pmc/articles/PMC6161806/ /pubmed/24756781 http://dx.doi.org/10.5301/jabfm.5000186 Text en © 2015 SAGE Publications http://www.creativecommons.org/licenses/by-nc-nd/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License (http://www.creativecommons.org/licenses/by-nc-nd/4.0/) which permits non-commercial use, reproduction and distribution of the work as published without adaptation or alteration, without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Boccafoschi, Francesca Rasponi, Marco Ramella, Martina Ferreira, Ana Marina Vesentini, Simone Cannas, Mario Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype |
title | Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype |
title_full | Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype |
title_fullStr | Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype |
title_full_unstemmed | Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype |
title_short | Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype |
title_sort | short-term effects of microstructured surfaces: role in cell differentiation toward a contractile phenotype |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161806/ https://www.ncbi.nlm.nih.gov/pubmed/24756781 http://dx.doi.org/10.5301/jabfm.5000186 |
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