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Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype

Cell adhesion plays a key role in cell behavior, in terms of migration, proliferation, differentiation and apoptosis. All of these events concur with tissue regeneration and remodeling mechanisms, integrating a complex network of intracellular signaling modules. Morphogenetic responses, which involv...

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Autores principales: Boccafoschi, Francesca, Rasponi, Marco, Ramella, Martina, Ferreira, Ana Marina, Vesentini, Simone, Cannas, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161806/
https://www.ncbi.nlm.nih.gov/pubmed/24756781
http://dx.doi.org/10.5301/jabfm.5000186
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author Boccafoschi, Francesca
Rasponi, Marco
Ramella, Martina
Ferreira, Ana Marina
Vesentini, Simone
Cannas, Mario
author_facet Boccafoschi, Francesca
Rasponi, Marco
Ramella, Martina
Ferreira, Ana Marina
Vesentini, Simone
Cannas, Mario
author_sort Boccafoschi, Francesca
collection PubMed
description Cell adhesion plays a key role in cell behavior, in terms of migration, proliferation, differentiation and apoptosis. All of these events concur with tissue regeneration and remodeling mechanisms, integrating a complex network of intracellular signaling modules. Morphogenetic responses, which involve changes in cell shape, proliferation and differentiation, are thought to be controlled by both biochemical and biophysical cues. Indeed, the extracellular matrix not only displays adhesive ligands necessary for cell adhesion but also plays an essential biomechanical role — responsible, for instance, for the acquisition of the contractile phenotype. The substrate topography around the forming tissues and the associated mechanical stresses that are generated regulate cellular morphology, proliferation and differentiation. Thus, the ability to tailor topographical features around cells can be a crucial design parameter in tissue engineering applications, inducing cells to exhibit the required performances. In this work, we designed micropillared substrates using highly spaced arrays (interspacing equal to 25 μm) to evaluate the effects of topography on C2C12 myoblasts' adhesion and differentiation. Optical and fluorescence microscopy images were used to observe cell adhesion, together with Western blot analysis on vinculin and focal adhesion kinase (FAK) expression, a protein highly involved in adhesive processes. Differentiation marker (Myf5, myogenin and myosin heavy chain [MHC]) expression was also studied, in relation to the effect of different substrate topographies on the enhancement of a contractile phenotype. Our results demonstrated that microstructured surfaces may play a key role in the regeneration of functional tissues.
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spelling pubmed-61618062018-10-11 Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype Boccafoschi, Francesca Rasponi, Marco Ramella, Martina Ferreira, Ana Marina Vesentini, Simone Cannas, Mario J Appl Biomater Funct Mater Original Article Cell adhesion plays a key role in cell behavior, in terms of migration, proliferation, differentiation and apoptosis. All of these events concur with tissue regeneration and remodeling mechanisms, integrating a complex network of intracellular signaling modules. Morphogenetic responses, which involve changes in cell shape, proliferation and differentiation, are thought to be controlled by both biochemical and biophysical cues. Indeed, the extracellular matrix not only displays adhesive ligands necessary for cell adhesion but also plays an essential biomechanical role — responsible, for instance, for the acquisition of the contractile phenotype. The substrate topography around the forming tissues and the associated mechanical stresses that are generated regulate cellular morphology, proliferation and differentiation. Thus, the ability to tailor topographical features around cells can be a crucial design parameter in tissue engineering applications, inducing cells to exhibit the required performances. In this work, we designed micropillared substrates using highly spaced arrays (interspacing equal to 25 μm) to evaluate the effects of topography on C2C12 myoblasts' adhesion and differentiation. Optical and fluorescence microscopy images were used to observe cell adhesion, together with Western blot analysis on vinculin and focal adhesion kinase (FAK) expression, a protein highly involved in adhesive processes. Differentiation marker (Myf5, myogenin and myosin heavy chain [MHC]) expression was also studied, in relation to the effect of different substrate topographies on the enhancement of a contractile phenotype. Our results demonstrated that microstructured surfaces may play a key role in the regeneration of functional tissues. SAGE Publications 2014-04-18 2015-07 /pmc/articles/PMC6161806/ /pubmed/24756781 http://dx.doi.org/10.5301/jabfm.5000186 Text en © 2015 SAGE Publications http://www.creativecommons.org/licenses/by-nc-nd/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License (http://www.creativecommons.org/licenses/by-nc-nd/4.0/) which permits non-commercial use, reproduction and distribution of the work as published without adaptation or alteration, without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Boccafoschi, Francesca
Rasponi, Marco
Ramella, Martina
Ferreira, Ana Marina
Vesentini, Simone
Cannas, Mario
Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype
title Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype
title_full Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype
title_fullStr Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype
title_full_unstemmed Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype
title_short Short-Term Effects of Microstructured Surfaces: Role in Cell Differentiation toward a Contractile Phenotype
title_sort short-term effects of microstructured surfaces: role in cell differentiation toward a contractile phenotype
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161806/
https://www.ncbi.nlm.nih.gov/pubmed/24756781
http://dx.doi.org/10.5301/jabfm.5000186
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