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Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens
Sheep colibacillosis is one of the most common bacterial diseases in large-scale sheep farms. In this study, we orally administered Escherichia coli F17 (E. coli F17) to lambs to obtain antagonistic and sensitive individuals. We used RNA-seq to screen for differential circRNAs in the spleens of both...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162294/ https://www.ncbi.nlm.nih.gov/pubmed/30266913 http://dx.doi.org/10.1038/s41598-018-31719-5 |
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author | Jin, Chengyan Bao, Jianjun Wang, Yue Chen, Weihao Zou, Shuangxia Wu, Tianyi Wang, Lihong Lv, Xiaoyang Gao, Wen Wang, Buzhong Zhu, Guoqiang Dai, Guojun Shi, Dongfang Sun, Wei |
author_facet | Jin, Chengyan Bao, Jianjun Wang, Yue Chen, Weihao Zou, Shuangxia Wu, Tianyi Wang, Lihong Lv, Xiaoyang Gao, Wen Wang, Buzhong Zhu, Guoqiang Dai, Guojun Shi, Dongfang Sun, Wei |
author_sort | Jin, Chengyan |
collection | PubMed |
description | Sheep colibacillosis is one of the most common bacterial diseases in large-scale sheep farms. In this study, we orally administered Escherichia coli F17 (E. coli F17) to lambs to obtain antagonistic and sensitive individuals. We used RNA-seq to screen for differential circRNAs in the spleens of both antagonist and sensitive individuals to explore the effect of circRNA on anti-diarrhoea in sheep. The results showed that 60 differentially expressed (DE) circRNAs were screened by RNA-seq in the spleen of antagonistic and sensitive lambs, among which 31 were up-regulated and 29 were down-regulated; q-PCR was used to validate the relative expression levels of six randomly selected circRNAs in antagonist and susceptible lambs and found to be consistent with the results of RNA-seq. Using Miranda analysis of circRNA-miRNA-mRNA interactions, we found a certain target relationship between 6 circRNAs, 5 miRNAs and 9 mRNAs. The relative expression levels of mRNA in antagonistic and sensitive lambs were verified by q-PCR and were consistent with the results of RNA-seq. This study explored the expression profile of circRNA in the spleen of an antagonistic and susceptible lamb with diarrhoea and found that differentially expressed circRNAs were helpful for determining how the lambs resist the pathogenesis of diarrhoea and provided a scientific basis for lambs to resist diarrhoea. |
format | Online Article Text |
id | pubmed-6162294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61622942018-10-02 Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens Jin, Chengyan Bao, Jianjun Wang, Yue Chen, Weihao Zou, Shuangxia Wu, Tianyi Wang, Lihong Lv, Xiaoyang Gao, Wen Wang, Buzhong Zhu, Guoqiang Dai, Guojun Shi, Dongfang Sun, Wei Sci Rep Article Sheep colibacillosis is one of the most common bacterial diseases in large-scale sheep farms. In this study, we orally administered Escherichia coli F17 (E. coli F17) to lambs to obtain antagonistic and sensitive individuals. We used RNA-seq to screen for differential circRNAs in the spleens of both antagonist and sensitive individuals to explore the effect of circRNA on anti-diarrhoea in sheep. The results showed that 60 differentially expressed (DE) circRNAs were screened by RNA-seq in the spleen of antagonistic and sensitive lambs, among which 31 were up-regulated and 29 were down-regulated; q-PCR was used to validate the relative expression levels of six randomly selected circRNAs in antagonist and susceptible lambs and found to be consistent with the results of RNA-seq. Using Miranda analysis of circRNA-miRNA-mRNA interactions, we found a certain target relationship between 6 circRNAs, 5 miRNAs and 9 mRNAs. The relative expression levels of mRNA in antagonistic and sensitive lambs were verified by q-PCR and were consistent with the results of RNA-seq. This study explored the expression profile of circRNA in the spleen of an antagonistic and susceptible lamb with diarrhoea and found that differentially expressed circRNAs were helpful for determining how the lambs resist the pathogenesis of diarrhoea and provided a scientific basis for lambs to resist diarrhoea. Nature Publishing Group UK 2018-09-28 /pmc/articles/PMC6162294/ /pubmed/30266913 http://dx.doi.org/10.1038/s41598-018-31719-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jin, Chengyan Bao, Jianjun Wang, Yue Chen, Weihao Zou, Shuangxia Wu, Tianyi Wang, Lihong Lv, Xiaoyang Gao, Wen Wang, Buzhong Zhu, Guoqiang Dai, Guojun Shi, Dongfang Sun, Wei Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens |
title | Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens |
title_full | Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens |
title_fullStr | Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens |
title_full_unstemmed | Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens |
title_short | Changes in circRNA expression profiles related to the antagonistic effects of Escherichia coli F17 in lamb spleens |
title_sort | changes in circrna expression profiles related to the antagonistic effects of escherichia coli f17 in lamb spleens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162294/ https://www.ncbi.nlm.nih.gov/pubmed/30266913 http://dx.doi.org/10.1038/s41598-018-31719-5 |
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