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miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162422/ https://www.ncbi.nlm.nih.gov/pubmed/30149628 http://dx.doi.org/10.3390/cancers10090289 |
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author | Nagesh, Prashanth K. B. Chowdhury, Pallabita Hatami, Elham Boya, Vijaya K. N. Kashyap, Vivek K. Khan, Sheema Hafeez, Bilal B. Chauhan, Subhash C. Jaggi, Meena Yallapu, Murali M. |
author_facet | Nagesh, Prashanth K. B. Chowdhury, Pallabita Hatami, Elham Boya, Vijaya K. N. Kashyap, Vivek K. Khan, Sheema Hafeez, Bilal B. Chauhan, Subhash C. Jaggi, Meena Yallapu, Murali M. |
author_sort | Nagesh, Prashanth K. B. |
collection | PubMed |
description | The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells. |
format | Online Article Text |
id | pubmed-6162422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61624222018-10-02 miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy Nagesh, Prashanth K. B. Chowdhury, Pallabita Hatami, Elham Boya, Vijaya K. N. Kashyap, Vivek K. Khan, Sheema Hafeez, Bilal B. Chauhan, Subhash C. Jaggi, Meena Yallapu, Murali M. Cancers (Basel) Article The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells. MDPI 2018-08-25 /pmc/articles/PMC6162422/ /pubmed/30149628 http://dx.doi.org/10.3390/cancers10090289 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nagesh, Prashanth K. B. Chowdhury, Pallabita Hatami, Elham Boya, Vijaya K. N. Kashyap, Vivek K. Khan, Sheema Hafeez, Bilal B. Chauhan, Subhash C. Jaggi, Meena Yallapu, Murali M. miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy |
title | miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy |
title_full | miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy |
title_fullStr | miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy |
title_full_unstemmed | miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy |
title_short | miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy |
title_sort | mirna-205 nanoformulation sensitizes prostate cancer cells to chemotherapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162422/ https://www.ncbi.nlm.nih.gov/pubmed/30149628 http://dx.doi.org/10.3390/cancers10090289 |
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