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miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy

The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical...

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Autores principales: Nagesh, Prashanth K. B., Chowdhury, Pallabita, Hatami, Elham, Boya, Vijaya K. N., Kashyap, Vivek K., Khan, Sheema, Hafeez, Bilal B., Chauhan, Subhash C., Jaggi, Meena, Yallapu, Murali M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162422/
https://www.ncbi.nlm.nih.gov/pubmed/30149628
http://dx.doi.org/10.3390/cancers10090289
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author Nagesh, Prashanth K. B.
Chowdhury, Pallabita
Hatami, Elham
Boya, Vijaya K. N.
Kashyap, Vivek K.
Khan, Sheema
Hafeez, Bilal B.
Chauhan, Subhash C.
Jaggi, Meena
Yallapu, Murali M.
author_facet Nagesh, Prashanth K. B.
Chowdhury, Pallabita
Hatami, Elham
Boya, Vijaya K. N.
Kashyap, Vivek K.
Khan, Sheema
Hafeez, Bilal B.
Chauhan, Subhash C.
Jaggi, Meena
Yallapu, Murali M.
author_sort Nagesh, Prashanth K. B.
collection PubMed
description The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells.
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spelling pubmed-61624222018-10-02 miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy Nagesh, Prashanth K. B. Chowdhury, Pallabita Hatami, Elham Boya, Vijaya K. N. Kashyap, Vivek K. Khan, Sheema Hafeez, Bilal B. Chauhan, Subhash C. Jaggi, Meena Yallapu, Murali M. Cancers (Basel) Article The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells. MDPI 2018-08-25 /pmc/articles/PMC6162422/ /pubmed/30149628 http://dx.doi.org/10.3390/cancers10090289 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nagesh, Prashanth K. B.
Chowdhury, Pallabita
Hatami, Elham
Boya, Vijaya K. N.
Kashyap, Vivek K.
Khan, Sheema
Hafeez, Bilal B.
Chauhan, Subhash C.
Jaggi, Meena
Yallapu, Murali M.
miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
title miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
title_full miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
title_fullStr miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
title_full_unstemmed miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
title_short miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy
title_sort mirna-205 nanoformulation sensitizes prostate cancer cells to chemotherapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162422/
https://www.ncbi.nlm.nih.gov/pubmed/30149628
http://dx.doi.org/10.3390/cancers10090289
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