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Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
The World Health Organization plans to eliminate hepatitis B and C Infections by 2030. Therefore, there is a need to study and understand hepatitis B virus (HBV) epidemiology and viral evolution further, including evaluating occult (HBsAg-negative) HBV infection (OBI), given that such infections are...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162474/ https://www.ncbi.nlm.nih.gov/pubmed/30205537 http://dx.doi.org/10.3390/genes9090453 |
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author | Anderson, Motswedi Choga, Wonderful Tatenda Moyo, Sikhulile Bell, Trevor Graham Mbangiwa, Tshepiso Phinius, Bonolo Bonita Bhebhe, Lynnette Sebunya, Theresa Kibirige Lockman, Shahin Marlink, Richard Kramvis, Anna Essex, Max Musonda, Rosemary Mubanga Blackard, Jason Tory Gaseitsiwe, Simani |
author_facet | Anderson, Motswedi Choga, Wonderful Tatenda Moyo, Sikhulile Bell, Trevor Graham Mbangiwa, Tshepiso Phinius, Bonolo Bonita Bhebhe, Lynnette Sebunya, Theresa Kibirige Lockman, Shahin Marlink, Richard Kramvis, Anna Essex, Max Musonda, Rosemary Mubanga Blackard, Jason Tory Gaseitsiwe, Simani |
author_sort | Anderson, Motswedi |
collection | PubMed |
description | The World Health Organization plans to eliminate hepatitis B and C Infections by 2030. Therefore, there is a need to study and understand hepatitis B virus (HBV) epidemiology and viral evolution further, including evaluating occult (HBsAg-negative) HBV infection (OBI), given that such infections are frequently undiagnosed and rarely treated. We aimed to molecularly characterize HBV genomes from 108 individuals co-infected with human immunodeficiency virus (HIV) and chronic hepatitis B (CHB) or OBI identified from previous HIV studies conducted in Botswana from 2009 to 2012. Full-length (3.2 kb) and nearly full-length (~3 kb) genomes were amplified by nested polymerase chain reaction (PCR). Sequences from OBI participants were compared to sequences from CHB participants and GenBank references to identify OBI-unique mutations. HBV genomes from 50 (25 CHB and 25 OBI) individuals were successfully genotyped. Among OBI participants, subgenotype A1 was identified in 12 (48%), D3 in 12 (48%), and E in 1 (4%). A similar genotype distribution was observed in CHB participants. Whole HBV genome sequences from Botswana, representing OBI and CHB, were compared for the first time. There were 43 OBI-unique mutations, of which 26 were novel. Future studies using larger sample sizes and functional analysis of OBI-unique mutations are warranted. |
format | Online Article Text |
id | pubmed-6162474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61624742018-10-10 Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana Anderson, Motswedi Choga, Wonderful Tatenda Moyo, Sikhulile Bell, Trevor Graham Mbangiwa, Tshepiso Phinius, Bonolo Bonita Bhebhe, Lynnette Sebunya, Theresa Kibirige Lockman, Shahin Marlink, Richard Kramvis, Anna Essex, Max Musonda, Rosemary Mubanga Blackard, Jason Tory Gaseitsiwe, Simani Genes (Basel) Article The World Health Organization plans to eliminate hepatitis B and C Infections by 2030. Therefore, there is a need to study and understand hepatitis B virus (HBV) epidemiology and viral evolution further, including evaluating occult (HBsAg-negative) HBV infection (OBI), given that such infections are frequently undiagnosed and rarely treated. We aimed to molecularly characterize HBV genomes from 108 individuals co-infected with human immunodeficiency virus (HIV) and chronic hepatitis B (CHB) or OBI identified from previous HIV studies conducted in Botswana from 2009 to 2012. Full-length (3.2 kb) and nearly full-length (~3 kb) genomes were amplified by nested polymerase chain reaction (PCR). Sequences from OBI participants were compared to sequences from CHB participants and GenBank references to identify OBI-unique mutations. HBV genomes from 50 (25 CHB and 25 OBI) individuals were successfully genotyped. Among OBI participants, subgenotype A1 was identified in 12 (48%), D3 in 12 (48%), and E in 1 (4%). A similar genotype distribution was observed in CHB participants. Whole HBV genome sequences from Botswana, representing OBI and CHB, were compared for the first time. There were 43 OBI-unique mutations, of which 26 were novel. Future studies using larger sample sizes and functional analysis of OBI-unique mutations are warranted. MDPI 2018-09-07 /pmc/articles/PMC6162474/ /pubmed/30205537 http://dx.doi.org/10.3390/genes9090453 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Anderson, Motswedi Choga, Wonderful Tatenda Moyo, Sikhulile Bell, Trevor Graham Mbangiwa, Tshepiso Phinius, Bonolo Bonita Bhebhe, Lynnette Sebunya, Theresa Kibirige Lockman, Shahin Marlink, Richard Kramvis, Anna Essex, Max Musonda, Rosemary Mubanga Blackard, Jason Tory Gaseitsiwe, Simani Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana |
title | Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana |
title_full | Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana |
title_fullStr | Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana |
title_full_unstemmed | Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana |
title_short | Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana |
title_sort | molecular characterization of near full-length genomes of hepatitis b virus isolated from predominantly hiv infected individuals in botswana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162474/ https://www.ncbi.nlm.nih.gov/pubmed/30205537 http://dx.doi.org/10.3390/genes9090453 |
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