Cargando…

Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana

The World Health Organization plans to eliminate hepatitis B and C Infections by 2030. Therefore, there is a need to study and understand hepatitis B virus (HBV) epidemiology and viral evolution further, including evaluating occult (HBsAg-negative) HBV infection (OBI), given that such infections are...

Descripción completa

Detalles Bibliográficos
Autores principales: Anderson, Motswedi, Choga, Wonderful Tatenda, Moyo, Sikhulile, Bell, Trevor Graham, Mbangiwa, Tshepiso, Phinius, Bonolo Bonita, Bhebhe, Lynnette, Sebunya, Theresa Kibirige, Lockman, Shahin, Marlink, Richard, Kramvis, Anna, Essex, Max, Musonda, Rosemary Mubanga, Blackard, Jason Tory, Gaseitsiwe, Simani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162474/
https://www.ncbi.nlm.nih.gov/pubmed/30205537
http://dx.doi.org/10.3390/genes9090453
_version_ 1783359154831753216
author Anderson, Motswedi
Choga, Wonderful Tatenda
Moyo, Sikhulile
Bell, Trevor Graham
Mbangiwa, Tshepiso
Phinius, Bonolo Bonita
Bhebhe, Lynnette
Sebunya, Theresa Kibirige
Lockman, Shahin
Marlink, Richard
Kramvis, Anna
Essex, Max
Musonda, Rosemary Mubanga
Blackard, Jason Tory
Gaseitsiwe, Simani
author_facet Anderson, Motswedi
Choga, Wonderful Tatenda
Moyo, Sikhulile
Bell, Trevor Graham
Mbangiwa, Tshepiso
Phinius, Bonolo Bonita
Bhebhe, Lynnette
Sebunya, Theresa Kibirige
Lockman, Shahin
Marlink, Richard
Kramvis, Anna
Essex, Max
Musonda, Rosemary Mubanga
Blackard, Jason Tory
Gaseitsiwe, Simani
author_sort Anderson, Motswedi
collection PubMed
description The World Health Organization plans to eliminate hepatitis B and C Infections by 2030. Therefore, there is a need to study and understand hepatitis B virus (HBV) epidemiology and viral evolution further, including evaluating occult (HBsAg-negative) HBV infection (OBI), given that such infections are frequently undiagnosed and rarely treated. We aimed to molecularly characterize HBV genomes from 108 individuals co-infected with human immunodeficiency virus (HIV) and chronic hepatitis B (CHB) or OBI identified from previous HIV studies conducted in Botswana from 2009 to 2012. Full-length (3.2 kb) and nearly full-length (~3 kb) genomes were amplified by nested polymerase chain reaction (PCR). Sequences from OBI participants were compared to sequences from CHB participants and GenBank references to identify OBI-unique mutations. HBV genomes from 50 (25 CHB and 25 OBI) individuals were successfully genotyped. Among OBI participants, subgenotype A1 was identified in 12 (48%), D3 in 12 (48%), and E in 1 (4%). A similar genotype distribution was observed in CHB participants. Whole HBV genome sequences from Botswana, representing OBI and CHB, were compared for the first time. There were 43 OBI-unique mutations, of which 26 were novel. Future studies using larger sample sizes and functional analysis of OBI-unique mutations are warranted.
format Online
Article
Text
id pubmed-6162474
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61624742018-10-10 Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana Anderson, Motswedi Choga, Wonderful Tatenda Moyo, Sikhulile Bell, Trevor Graham Mbangiwa, Tshepiso Phinius, Bonolo Bonita Bhebhe, Lynnette Sebunya, Theresa Kibirige Lockman, Shahin Marlink, Richard Kramvis, Anna Essex, Max Musonda, Rosemary Mubanga Blackard, Jason Tory Gaseitsiwe, Simani Genes (Basel) Article The World Health Organization plans to eliminate hepatitis B and C Infections by 2030. Therefore, there is a need to study and understand hepatitis B virus (HBV) epidemiology and viral evolution further, including evaluating occult (HBsAg-negative) HBV infection (OBI), given that such infections are frequently undiagnosed and rarely treated. We aimed to molecularly characterize HBV genomes from 108 individuals co-infected with human immunodeficiency virus (HIV) and chronic hepatitis B (CHB) or OBI identified from previous HIV studies conducted in Botswana from 2009 to 2012. Full-length (3.2 kb) and nearly full-length (~3 kb) genomes were amplified by nested polymerase chain reaction (PCR). Sequences from OBI participants were compared to sequences from CHB participants and GenBank references to identify OBI-unique mutations. HBV genomes from 50 (25 CHB and 25 OBI) individuals were successfully genotyped. Among OBI participants, subgenotype A1 was identified in 12 (48%), D3 in 12 (48%), and E in 1 (4%). A similar genotype distribution was observed in CHB participants. Whole HBV genome sequences from Botswana, representing OBI and CHB, were compared for the first time. There were 43 OBI-unique mutations, of which 26 were novel. Future studies using larger sample sizes and functional analysis of OBI-unique mutations are warranted. MDPI 2018-09-07 /pmc/articles/PMC6162474/ /pubmed/30205537 http://dx.doi.org/10.3390/genes9090453 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Anderson, Motswedi
Choga, Wonderful Tatenda
Moyo, Sikhulile
Bell, Trevor Graham
Mbangiwa, Tshepiso
Phinius, Bonolo Bonita
Bhebhe, Lynnette
Sebunya, Theresa Kibirige
Lockman, Shahin
Marlink, Richard
Kramvis, Anna
Essex, Max
Musonda, Rosemary Mubanga
Blackard, Jason Tory
Gaseitsiwe, Simani
Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
title Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
title_full Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
title_fullStr Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
title_full_unstemmed Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
title_short Molecular Characterization of Near Full-Length Genomes of Hepatitis B Virus Isolated from Predominantly HIV Infected Individuals in Botswana
title_sort molecular characterization of near full-length genomes of hepatitis b virus isolated from predominantly hiv infected individuals in botswana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162474/
https://www.ncbi.nlm.nih.gov/pubmed/30205537
http://dx.doi.org/10.3390/genes9090453
work_keys_str_mv AT andersonmotswedi molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT chogawonderfultatenda molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT moyosikhulile molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT belltrevorgraham molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT mbangiwatshepiso molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT phiniusbonolobonita molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT bhebhelynnette molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT sebunyatheresakibirige molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT lockmanshahin molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT marlinkrichard molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT kramvisanna molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT essexmax molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT musondarosemarymubanga molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT blackardjasontory molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana
AT gaseitsiwesimani molecularcharacterizationofnearfulllengthgenomesofhepatitisbvirusisolatedfrompredominantlyhivinfectedindividualsinbotswana