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Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups

The molecular detection of Pneumocystis jirovecii is an important therapy-relevant tool in microbiological diagnostics. However, the quantification of this pathogen in the past has revealed discordant results depending on the target gene. As the clinical variety of P. jirovecii infections ranges bet...

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Autores principales: Dunaiski, Cara Mia, Janssen, Lena, Erzinger, Hannah, Pieper, Monika, Damaschek, Sarah, Schildgen, Oliver, Schildgen, Verena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162491/
https://www.ncbi.nlm.nih.gov/pubmed/29996561
http://dx.doi.org/10.3390/jof4030084
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author Dunaiski, Cara Mia
Janssen, Lena
Erzinger, Hannah
Pieper, Monika
Damaschek, Sarah
Schildgen, Oliver
Schildgen, Verena
author_facet Dunaiski, Cara Mia
Janssen, Lena
Erzinger, Hannah
Pieper, Monika
Damaschek, Sarah
Schildgen, Oliver
Schildgen, Verena
author_sort Dunaiski, Cara Mia
collection PubMed
description The molecular detection of Pneumocystis jirovecii is an important therapy-relevant tool in microbiological diagnostics. However, the quantification of this pathogen in the past has revealed discordant results depending on the target gene. As the clinical variety of P. jirovecii infections ranges between life-threatening infections and symptom-free colonization, the question arises if qPCRs are reliable tools for quantitative diagnostics of P. jirovecii. P. jirovecii positive BALs were quantitatively tested for the copy numbers of one mitochondrial (COX-1) and two nuclear single-copy genes (KEX1 and DHPS) compared to the mitochondrial large subunit (mtLSU) by qPCR. Independent of the overall mtLSU copy number P. jirovecii clustered into distinct groups based on the ratio patterns of the respective qPCRs. This study, which compared different mitochondrial to nuclear gene ratio patterns of independent patients, shows that the mtLSU gene represents a highly sensitive qPCR tool for the detection of P. jirovecii, but does not display a reliable target for absolute quantification.
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spelling pubmed-61624912018-10-09 Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups Dunaiski, Cara Mia Janssen, Lena Erzinger, Hannah Pieper, Monika Damaschek, Sarah Schildgen, Oliver Schildgen, Verena J Fungi (Basel) Article The molecular detection of Pneumocystis jirovecii is an important therapy-relevant tool in microbiological diagnostics. However, the quantification of this pathogen in the past has revealed discordant results depending on the target gene. As the clinical variety of P. jirovecii infections ranges between life-threatening infections and symptom-free colonization, the question arises if qPCRs are reliable tools for quantitative diagnostics of P. jirovecii. P. jirovecii positive BALs were quantitatively tested for the copy numbers of one mitochondrial (COX-1) and two nuclear single-copy genes (KEX1 and DHPS) compared to the mitochondrial large subunit (mtLSU) by qPCR. Independent of the overall mtLSU copy number P. jirovecii clustered into distinct groups based on the ratio patterns of the respective qPCRs. This study, which compared different mitochondrial to nuclear gene ratio patterns of independent patients, shows that the mtLSU gene represents a highly sensitive qPCR tool for the detection of P. jirovecii, but does not display a reliable target for absolute quantification. MDPI 2018-07-10 /pmc/articles/PMC6162491/ /pubmed/29996561 http://dx.doi.org/10.3390/jof4030084 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dunaiski, Cara Mia
Janssen, Lena
Erzinger, Hannah
Pieper, Monika
Damaschek, Sarah
Schildgen, Oliver
Schildgen, Verena
Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups
title Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups
title_full Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups
title_fullStr Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups
title_full_unstemmed Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups
title_short Inter-Specimen Imbalance of Mitochondrial Gene Copy Numbers Predicts Clustering of Pneumocystis jirovecii Isolates in Distinct Subgroups
title_sort inter-specimen imbalance of mitochondrial gene copy numbers predicts clustering of pneumocystis jirovecii isolates in distinct subgroups
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162491/
https://www.ncbi.nlm.nih.gov/pubmed/29996561
http://dx.doi.org/10.3390/jof4030084
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