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Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells
Classical Hodgkin’s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information was availabl...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162537/ https://www.ncbi.nlm.nih.gov/pubmed/30149530 http://dx.doi.org/10.3390/cancers10090286 |
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author | Contu, Fabio Rangel-Pozzo, Aline Trokajlo, Peter Wark, Landon Klewes, Ludger Johnson, Nathalie A. Petrogiannis-Haliotis, Tina Gartner, John G. Garini, Yuval Vanni, Roberta Knecht, Hans Mai, Sabine |
author_facet | Contu, Fabio Rangel-Pozzo, Aline Trokajlo, Peter Wark, Landon Klewes, Ludger Johnson, Nathalie A. Petrogiannis-Haliotis, Tina Gartner, John G. Garini, Yuval Vanni, Roberta Knecht, Hans Mai, Sabine |
author_sort | Contu, Fabio |
collection | PubMed |
description | Classical Hodgkin’s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information was available about the three-dimensional (3D) expression patterns of lamin A/C in H and RS cells, we analyzed the 3D spatial organization of lamin in such cells, using 3D fluorescent microscopy. H and RS cells from cHL derived cell lines stained positive for lamin A/C, in contrast to peripheral blood lymphocytes (PBLs), in which the lamin A/C protein was not detected or weak, although its presence could be transiently increased with lymphocyte activation by lipopolysaccharide (LPS). Most importantly, in H and RS cells, the regular homogeneous and spherically shaped lamin A/C pattern, identified in activated lymphocytes, was absent. Instead, in H and RS cells, lamin staining showed internal lamin A/C structures, subdividing the nuclei into two or more smaller compartments. Analysis of pre-treatment cHL patients’ samples replicated the lamin patterns identified in cHL cell lines. We conclude that the investigation of lamin A/C protein could be a useful tool for understanding nuclear remodeling in cHL. |
format | Online Article Text |
id | pubmed-6162537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61625372018-10-02 Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells Contu, Fabio Rangel-Pozzo, Aline Trokajlo, Peter Wark, Landon Klewes, Ludger Johnson, Nathalie A. Petrogiannis-Haliotis, Tina Gartner, John G. Garini, Yuval Vanni, Roberta Knecht, Hans Mai, Sabine Cancers (Basel) Article Classical Hodgkin’s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information was available about the three-dimensional (3D) expression patterns of lamin A/C in H and RS cells, we analyzed the 3D spatial organization of lamin in such cells, using 3D fluorescent microscopy. H and RS cells from cHL derived cell lines stained positive for lamin A/C, in contrast to peripheral blood lymphocytes (PBLs), in which the lamin A/C protein was not detected or weak, although its presence could be transiently increased with lymphocyte activation by lipopolysaccharide (LPS). Most importantly, in H and RS cells, the regular homogeneous and spherically shaped lamin A/C pattern, identified in activated lymphocytes, was absent. Instead, in H and RS cells, lamin staining showed internal lamin A/C structures, subdividing the nuclei into two or more smaller compartments. Analysis of pre-treatment cHL patients’ samples replicated the lamin patterns identified in cHL cell lines. We conclude that the investigation of lamin A/C protein could be a useful tool for understanding nuclear remodeling in cHL. MDPI 2018-08-24 /pmc/articles/PMC6162537/ /pubmed/30149530 http://dx.doi.org/10.3390/cancers10090286 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Contu, Fabio Rangel-Pozzo, Aline Trokajlo, Peter Wark, Landon Klewes, Ludger Johnson, Nathalie A. Petrogiannis-Haliotis, Tina Gartner, John G. Garini, Yuval Vanni, Roberta Knecht, Hans Mai, Sabine Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
title | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
title_full | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
title_fullStr | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
title_full_unstemmed | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
title_short | Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells |
title_sort | distinct 3d structural patterns of lamin a/c expression in hodgkin and reed-sternberg cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162537/ https://www.ncbi.nlm.nih.gov/pubmed/30149530 http://dx.doi.org/10.3390/cancers10090286 |
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