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Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts

Diagnostic tests for arachnid accidents remain unavailable for patients and clinicians. Together with snakes, these accidents are still a global medical concern, and are recognized as neglected tropical issues. Due to arachnid toxins’ fast mechanism of action, quick detection and quantification of v...

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Autores principales: Dias-Lopes, Camila, Paiva, Ana Luiza, Guerra-Duarte, Clara, Molina, Franck, Felicori, Liza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162545/
https://www.ncbi.nlm.nih.gov/pubmed/30201918
http://dx.doi.org/10.3390/toxins10090365
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author Dias-Lopes, Camila
Paiva, Ana Luiza
Guerra-Duarte, Clara
Molina, Franck
Felicori, Liza
author_facet Dias-Lopes, Camila
Paiva, Ana Luiza
Guerra-Duarte, Clara
Molina, Franck
Felicori, Liza
author_sort Dias-Lopes, Camila
collection PubMed
description Diagnostic tests for arachnid accidents remain unavailable for patients and clinicians. Together with snakes, these accidents are still a global medical concern, and are recognized as neglected tropical issues. Due to arachnid toxins’ fast mechanism of action, quick detection and quantification of venom is required to accelerate treatment decisions, rationalize therapy, and reduce costs and patient risks. This review aims to understand the current limitations for arachnid venom identification and quantification in biological samples. We benchmarked the already existing initiatives regarding test requirements (sample or biomarkers of choice), performances (time, detection limit, sensitivity and specificity) and their validation (on animal models or on samples from envenomed humans). Our analysis outlines unmet needs for improving diagnosis and consequently treatment of arachnid accidents. Hence, based on lessons from past attempts, we propose a road map for raising best practice guidelines, leading to recommendations for future progress in the development of arachnid diagnostic assays.
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spelling pubmed-61625452018-10-03 Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts Dias-Lopes, Camila Paiva, Ana Luiza Guerra-Duarte, Clara Molina, Franck Felicori, Liza Toxins (Basel) Review Diagnostic tests for arachnid accidents remain unavailable for patients and clinicians. Together with snakes, these accidents are still a global medical concern, and are recognized as neglected tropical issues. Due to arachnid toxins’ fast mechanism of action, quick detection and quantification of venom is required to accelerate treatment decisions, rationalize therapy, and reduce costs and patient risks. This review aims to understand the current limitations for arachnid venom identification and quantification in biological samples. We benchmarked the already existing initiatives regarding test requirements (sample or biomarkers of choice), performances (time, detection limit, sensitivity and specificity) and their validation (on animal models or on samples from envenomed humans). Our analysis outlines unmet needs for improving diagnosis and consequently treatment of arachnid accidents. Hence, based on lessons from past attempts, we propose a road map for raising best practice guidelines, leading to recommendations for future progress in the development of arachnid diagnostic assays. MDPI 2018-09-10 /pmc/articles/PMC6162545/ /pubmed/30201918 http://dx.doi.org/10.3390/toxins10090365 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Dias-Lopes, Camila
Paiva, Ana Luiza
Guerra-Duarte, Clara
Molina, Franck
Felicori, Liza
Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts
title Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts
title_full Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts
title_fullStr Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts
title_full_unstemmed Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts
title_short Venomous Arachnid Diagnostic Assays, Lessons from Past Attempts
title_sort venomous arachnid diagnostic assays, lessons from past attempts
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162545/
https://www.ncbi.nlm.nih.gov/pubmed/30201918
http://dx.doi.org/10.3390/toxins10090365
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