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Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis?
Tissue from 13 autopsy cases with invasive gastrointestinal candidiasis was studied for the binding of the pentraxins, C-reactive protein (CRP), pentraxin 3 (PTX3), and serum amyloid P component (SAP) to fungal surfaces. Invasive candidal infection was demonstrated using a hematoxylin and eosin stai...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162546/ https://www.ncbi.nlm.nih.gov/pubmed/30227609 http://dx.doi.org/10.3390/jof4030111 |
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author | Golconda, Umamaheshwari Sobonya, Richard E. Klotz, Stephen A. |
author_facet | Golconda, Umamaheshwari Sobonya, Richard E. Klotz, Stephen A. |
author_sort | Golconda, Umamaheshwari |
collection | PubMed |
description | Tissue from 13 autopsy cases with invasive gastrointestinal candidiasis was studied for the binding of the pentraxins, C-reactive protein (CRP), pentraxin 3 (PTX3), and serum amyloid P component (SAP) to fungal surfaces. Invasive candidal infection was demonstrated using a hematoxylin and eosin stain and a Gomori methenamine silver stain (GMS). Immunohistochemistry was performed with CRP and PTX3 monoclonal antibodies and did not demonstrate CRP or PTX3 bound to fungi (0 of 13 cases), although CRP was extensively deposited on human tissue. A polyclonal antibody to SAP showed that SAP was bound to fungi in 12 of 13 cases. Although all three pentraxins have been reported to bind to fungi or bacteria, only SAP was bound to filamentous and yeast forms of Candida in human tissue, as detected by immunohistochemistry. SAP was abundantly present on fungi and may have affected the host innate immune response to the invading fungi. |
format | Online Article Text |
id | pubmed-6162546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61625462018-10-09 Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis? Golconda, Umamaheshwari Sobonya, Richard E. Klotz, Stephen A. J Fungi (Basel) Article Tissue from 13 autopsy cases with invasive gastrointestinal candidiasis was studied for the binding of the pentraxins, C-reactive protein (CRP), pentraxin 3 (PTX3), and serum amyloid P component (SAP) to fungal surfaces. Invasive candidal infection was demonstrated using a hematoxylin and eosin stain and a Gomori methenamine silver stain (GMS). Immunohistochemistry was performed with CRP and PTX3 monoclonal antibodies and did not demonstrate CRP or PTX3 bound to fungi (0 of 13 cases), although CRP was extensively deposited on human tissue. A polyclonal antibody to SAP showed that SAP was bound to fungi in 12 of 13 cases. Although all three pentraxins have been reported to bind to fungi or bacteria, only SAP was bound to filamentous and yeast forms of Candida in human tissue, as detected by immunohistochemistry. SAP was abundantly present on fungi and may have affected the host innate immune response to the invading fungi. MDPI 2018-09-17 /pmc/articles/PMC6162546/ /pubmed/30227609 http://dx.doi.org/10.3390/jof4030111 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Golconda, Umamaheshwari Sobonya, Richard E. Klotz, Stephen A. Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis? |
title | Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis? |
title_full | Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis? |
title_fullStr | Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis? |
title_full_unstemmed | Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis? |
title_short | Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis? |
title_sort | do pentraxins bind to fungi in invasive human gastrointestinal candidiasis? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162546/ https://www.ncbi.nlm.nih.gov/pubmed/30227609 http://dx.doi.org/10.3390/jof4030111 |
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