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Impact of Fungal MAPK Pathway Targets on the Cell Wall
The fungal cell wall is an extracellular organelle that provides structure and protection to cells. The cell wall also influences the interactions of cells with each other and surfaces. The cell wall can be reorganized in response to changing environmental conditions and different types of stress. S...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162559/ https://www.ncbi.nlm.nih.gov/pubmed/30096860 http://dx.doi.org/10.3390/jof4030093 |
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author | Chow, Jacky Notaro, Marysa Prabhakar, Aditi Free, Stephen J. Cullen, Paul J. |
author_facet | Chow, Jacky Notaro, Marysa Prabhakar, Aditi Free, Stephen J. Cullen, Paul J. |
author_sort | Chow, Jacky |
collection | PubMed |
description | The fungal cell wall is an extracellular organelle that provides structure and protection to cells. The cell wall also influences the interactions of cells with each other and surfaces. The cell wall can be reorganized in response to changing environmental conditions and different types of stress. Signaling pathways control the remodeling of the cell wall through target proteins that are in many cases not well defined. The Mitogen Activated Protein Kinase pathway that controls filamentous growth in yeast (fMAPK) was required for normal growth in media containing the cell wall perturbing agent Calcofluor White (CFW). A mass spectrometry (MASS-SPEC) approach and analysis of expression profiling data identified cell wall proteins and modifying enzymes whose levels were influenced by the fMAPK pathway. These include Flo11p, Flo10p, Tip1p, Pry2p and the mannosyltransferase, Och1p. Cells lacking Flo11p or Och1p were sensitive to CFW. The identification of cell wall proteins controlled by a MAPK pathway may provide insights into how signaling pathways regulate the cell wall. |
format | Online Article Text |
id | pubmed-6162559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61625592018-10-09 Impact of Fungal MAPK Pathway Targets on the Cell Wall Chow, Jacky Notaro, Marysa Prabhakar, Aditi Free, Stephen J. Cullen, Paul J. J Fungi (Basel) Article The fungal cell wall is an extracellular organelle that provides structure and protection to cells. The cell wall also influences the interactions of cells with each other and surfaces. The cell wall can be reorganized in response to changing environmental conditions and different types of stress. Signaling pathways control the remodeling of the cell wall through target proteins that are in many cases not well defined. The Mitogen Activated Protein Kinase pathway that controls filamentous growth in yeast (fMAPK) was required for normal growth in media containing the cell wall perturbing agent Calcofluor White (CFW). A mass spectrometry (MASS-SPEC) approach and analysis of expression profiling data identified cell wall proteins and modifying enzymes whose levels were influenced by the fMAPK pathway. These include Flo11p, Flo10p, Tip1p, Pry2p and the mannosyltransferase, Och1p. Cells lacking Flo11p or Och1p were sensitive to CFW. The identification of cell wall proteins controlled by a MAPK pathway may provide insights into how signaling pathways regulate the cell wall. MDPI 2018-08-09 /pmc/articles/PMC6162559/ /pubmed/30096860 http://dx.doi.org/10.3390/jof4030093 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chow, Jacky Notaro, Marysa Prabhakar, Aditi Free, Stephen J. Cullen, Paul J. Impact of Fungal MAPK Pathway Targets on the Cell Wall |
title | Impact of Fungal MAPK Pathway Targets on the Cell Wall |
title_full | Impact of Fungal MAPK Pathway Targets on the Cell Wall |
title_fullStr | Impact of Fungal MAPK Pathway Targets on the Cell Wall |
title_full_unstemmed | Impact of Fungal MAPK Pathway Targets on the Cell Wall |
title_short | Impact of Fungal MAPK Pathway Targets on the Cell Wall |
title_sort | impact of fungal mapk pathway targets on the cell wall |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162559/ https://www.ncbi.nlm.nih.gov/pubmed/30096860 http://dx.doi.org/10.3390/jof4030093 |
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