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Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens

In the past decade, Galleria mellonella (wax moth) larvae have become widely used as a non-mammalian infection model. However, the full potential of this infection model has yet to be realised, limited by the variable quality of larvae used and the lack of standardised procedures. Here, we review la...

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Autores principales: Champion, Olivia L., Titball, Richard W., Bates, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162639/
https://www.ncbi.nlm.nih.gov/pubmed/30200639
http://dx.doi.org/10.3390/jof4030108
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author Champion, Olivia L.
Titball, Richard W.
Bates, Steven
author_facet Champion, Olivia L.
Titball, Richard W.
Bates, Steven
author_sort Champion, Olivia L.
collection PubMed
description In the past decade, Galleria mellonella (wax moth) larvae have become widely used as a non-mammalian infection model. However, the full potential of this infection model has yet to be realised, limited by the variable quality of larvae used and the lack of standardised procedures. Here, we review larvae suitable for research, protocols for dosing larvae, and methods for scoring illness in larvae infected with fungal pathogens. The development of standardised protocols for carrying out our experimental work will allow high throughput screens to be developed, changing the way in which we evaluate panels of mutants and strains. It will also enable the in vivo screening of potential antimicrobials at an earlier stage in the research and development cycle.
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spelling pubmed-61626392018-10-09 Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens Champion, Olivia L. Titball, Richard W. Bates, Steven J Fungi (Basel) Review In the past decade, Galleria mellonella (wax moth) larvae have become widely used as a non-mammalian infection model. However, the full potential of this infection model has yet to be realised, limited by the variable quality of larvae used and the lack of standardised procedures. Here, we review larvae suitable for research, protocols for dosing larvae, and methods for scoring illness in larvae infected with fungal pathogens. The development of standardised protocols for carrying out our experimental work will allow high throughput screens to be developed, changing the way in which we evaluate panels of mutants and strains. It will also enable the in vivo screening of potential antimicrobials at an earlier stage in the research and development cycle. MDPI 2018-09-06 /pmc/articles/PMC6162639/ /pubmed/30200639 http://dx.doi.org/10.3390/jof4030108 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Champion, Olivia L.
Titball, Richard W.
Bates, Steven
Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens
title Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens
title_full Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens
title_fullStr Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens
title_full_unstemmed Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens
title_short Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens
title_sort standardization of g. mellonella larvae to provide reliable and reproducible results in the study of fungal pathogens
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162639/
https://www.ncbi.nlm.nih.gov/pubmed/30200639
http://dx.doi.org/10.3390/jof4030108
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