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Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth
Rodent models of liver tumorigenesis have reproducibly shown that dietary sugar intake is a powerful driver of liver tumor initiation and growth. In contrast, dietary sugar restriction with ketogenic diets or calorie restriction generally prevents liver tumor formation. Ketogenic diet is viewed posi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162796/ https://www.ncbi.nlm.nih.gov/pubmed/30189621 http://dx.doi.org/10.3390/cancers10090312 |
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author | Byrne, Frances L. Hargett, Stefan R. Lahiri, Sujoy Roy, R. Jack Berr, Stuart S. Caldwell, Stephen H. Hoehn, Kyle L. |
author_facet | Byrne, Frances L. Hargett, Stefan R. Lahiri, Sujoy Roy, R. Jack Berr, Stuart S. Caldwell, Stephen H. Hoehn, Kyle L. |
author_sort | Byrne, Frances L. |
collection | PubMed |
description | Rodent models of liver tumorigenesis have reproducibly shown that dietary sugar intake is a powerful driver of liver tumor initiation and growth. In contrast, dietary sugar restriction with ketogenic diets or calorie restriction generally prevents liver tumor formation. Ketogenic diet is viewed positively as a therapeutic adjuvant; however, most ketogenic diet studies described to date have been performed in prevention mode rather than treatment mode. Therefore, it remains unclear whether a ketogenic diet can be administered in late stages of disease to stall or reverse liver tumor growth. To model the clinically relevant treatment mode, we administered a ketogenic diet to mice after liver tumor initiation and monitored tumor growth by magnetic resonance imaging (MRI). Male C57BL/6 mice were injected with diethylnitrosamine (DEN) at 2 weeks of age and fed a chow diet until 39 weeks of age, when they underwent MRI imaging to detect liver tumors. Mice were then randomised into two groups and fed either a chow diet or switched to a ketogenic diet from 40–48 weeks of age. Serial MRIs were performed at 44 and 48 weeks of age. All mice had tumors at study completion and there were no differences in total tumor burden between diet groups. Although a ketogenic diet has marked protective effects against DEN-induced liver tumourigenesis in this mouse model, these data demonstrate that ketogenic diet cannot stop the progression of established liver tumors. |
format | Online Article Text |
id | pubmed-6162796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61627962018-10-02 Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth Byrne, Frances L. Hargett, Stefan R. Lahiri, Sujoy Roy, R. Jack Berr, Stuart S. Caldwell, Stephen H. Hoehn, Kyle L. Cancers (Basel) Brief Report Rodent models of liver tumorigenesis have reproducibly shown that dietary sugar intake is a powerful driver of liver tumor initiation and growth. In contrast, dietary sugar restriction with ketogenic diets or calorie restriction generally prevents liver tumor formation. Ketogenic diet is viewed positively as a therapeutic adjuvant; however, most ketogenic diet studies described to date have been performed in prevention mode rather than treatment mode. Therefore, it remains unclear whether a ketogenic diet can be administered in late stages of disease to stall or reverse liver tumor growth. To model the clinically relevant treatment mode, we administered a ketogenic diet to mice after liver tumor initiation and monitored tumor growth by magnetic resonance imaging (MRI). Male C57BL/6 mice were injected with diethylnitrosamine (DEN) at 2 weeks of age and fed a chow diet until 39 weeks of age, when they underwent MRI imaging to detect liver tumors. Mice were then randomised into two groups and fed either a chow diet or switched to a ketogenic diet from 40–48 weeks of age. Serial MRIs were performed at 44 and 48 weeks of age. All mice had tumors at study completion and there were no differences in total tumor burden between diet groups. Although a ketogenic diet has marked protective effects against DEN-induced liver tumourigenesis in this mouse model, these data demonstrate that ketogenic diet cannot stop the progression of established liver tumors. MDPI 2018-09-05 /pmc/articles/PMC6162796/ /pubmed/30189621 http://dx.doi.org/10.3390/cancers10090312 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Byrne, Frances L. Hargett, Stefan R. Lahiri, Sujoy Roy, R. Jack Berr, Stuart S. Caldwell, Stephen H. Hoehn, Kyle L. Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth |
title | Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth |
title_full | Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth |
title_fullStr | Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth |
title_full_unstemmed | Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth |
title_short | Serial MRI Imaging Reveals Minimal Impact of Ketogenic Diet on Established Liver Tumor Growth |
title_sort | serial mri imaging reveals minimal impact of ketogenic diet on established liver tumor growth |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162796/ https://www.ncbi.nlm.nih.gov/pubmed/30189621 http://dx.doi.org/10.3390/cancers10090312 |
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