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Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps

The use of -omics technologies allows for the characterization of snake venom composition at a fast rate and at high levels of detail. In the present study, we investigated the protein content of Red-headed Krait (Bungarus flaviceps) venom. This analysis revealed a high diversity of snake venom prot...

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Autores principales: Chapeaurouge, Alex, Silva, Andreza, Carvalho, Paulo, McCleary, Ryan J. R., Modahl, Cassandra Marie, Perales, Jonas, Kini, R. Manjunatha, Mackessy, Stephen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162843/
https://www.ncbi.nlm.nih.gov/pubmed/30217057
http://dx.doi.org/10.3390/toxins10090373
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author Chapeaurouge, Alex
Silva, Andreza
Carvalho, Paulo
McCleary, Ryan J. R.
Modahl, Cassandra Marie
Perales, Jonas
Kini, R. Manjunatha
Mackessy, Stephen P.
author_facet Chapeaurouge, Alex
Silva, Andreza
Carvalho, Paulo
McCleary, Ryan J. R.
Modahl, Cassandra Marie
Perales, Jonas
Kini, R. Manjunatha
Mackessy, Stephen P.
author_sort Chapeaurouge, Alex
collection PubMed
description The use of -omics technologies allows for the characterization of snake venom composition at a fast rate and at high levels of detail. In the present study, we investigated the protein content of Red-headed Krait (Bungarus flaviceps) venom. This analysis revealed a high diversity of snake venom protein families, as evidenced by high-throughput mass spectrometric analysis. We found all six venom protein families previously reported in a transcriptome study of the venom gland of B. flaviceps, including phospholipases A(2) (PLA(2)s), Kunitz-type serine proteinase inhibitors (KSPIs), three-finger toxins (3FTxs), cysteine-rich secretory proteins (CRISPs), snaclecs, and natriuretic peptides. A combined approach of automated database searches and de novo sequencing of tandem mass spectra, followed by sequence similarity searches, revealed the presence of 12 additional toxin families. De novo sequencing alone was able to identify 58 additional peptides, and this approach contributed significantly to the comprehensive description of the venom. Abundant protein families comprise 3FTxs (22.3%), KSPIs (19%), acetylcholinesterases (12.6%), PLA(2)s (11.9%), venom endothelial growth factors (VEGFs, 8.4%), nucleotidases (4.3%), and C-type lectin-like proteins (snaclecs, 3.3%); an additional 11 toxin families are present at significantly lower concentrations, including complement depleting factors, a family not previously detected in Bungarus venoms. The utility of a multifaceted approach toward unraveling the proteome of snake venoms, employed here, allowed detection of even minor venom components. This more in-depth knowledge of the composition of B. flaviceps venom facilitates a better understanding of snake venom molecular evolution, in turn contributing to more effective treatment of krait bites.
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spelling pubmed-61628432018-10-03 Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps Chapeaurouge, Alex Silva, Andreza Carvalho, Paulo McCleary, Ryan J. R. Modahl, Cassandra Marie Perales, Jonas Kini, R. Manjunatha Mackessy, Stephen P. Toxins (Basel) Article The use of -omics technologies allows for the characterization of snake venom composition at a fast rate and at high levels of detail. In the present study, we investigated the protein content of Red-headed Krait (Bungarus flaviceps) venom. This analysis revealed a high diversity of snake venom protein families, as evidenced by high-throughput mass spectrometric analysis. We found all six venom protein families previously reported in a transcriptome study of the venom gland of B. flaviceps, including phospholipases A(2) (PLA(2)s), Kunitz-type serine proteinase inhibitors (KSPIs), three-finger toxins (3FTxs), cysteine-rich secretory proteins (CRISPs), snaclecs, and natriuretic peptides. A combined approach of automated database searches and de novo sequencing of tandem mass spectra, followed by sequence similarity searches, revealed the presence of 12 additional toxin families. De novo sequencing alone was able to identify 58 additional peptides, and this approach contributed significantly to the comprehensive description of the venom. Abundant protein families comprise 3FTxs (22.3%), KSPIs (19%), acetylcholinesterases (12.6%), PLA(2)s (11.9%), venom endothelial growth factors (VEGFs, 8.4%), nucleotidases (4.3%), and C-type lectin-like proteins (snaclecs, 3.3%); an additional 11 toxin families are present at significantly lower concentrations, including complement depleting factors, a family not previously detected in Bungarus venoms. The utility of a multifaceted approach toward unraveling the proteome of snake venoms, employed here, allowed detection of even minor venom components. This more in-depth knowledge of the composition of B. flaviceps venom facilitates a better understanding of snake venom molecular evolution, in turn contributing to more effective treatment of krait bites. MDPI 2018-09-13 /pmc/articles/PMC6162843/ /pubmed/30217057 http://dx.doi.org/10.3390/toxins10090373 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chapeaurouge, Alex
Silva, Andreza
Carvalho, Paulo
McCleary, Ryan J. R.
Modahl, Cassandra Marie
Perales, Jonas
Kini, R. Manjunatha
Mackessy, Stephen P.
Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps
title Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps
title_full Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps
title_fullStr Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps
title_full_unstemmed Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps
title_short Proteomic Deep Mining the Venom of the Red-Headed Krait, Bungarus flaviceps
title_sort proteomic deep mining the venom of the red-headed krait, bungarus flaviceps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162843/
https://www.ncbi.nlm.nih.gov/pubmed/30217057
http://dx.doi.org/10.3390/toxins10090373
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