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The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer

Microsatellite instability (MSI) is a distinct molecular subtype of gastric cancer. In recent years, the clinical consequences of MSI and the therapeutic opportunities to target this peculiar cancer subtype became evident. However, despite the importance of MSI for the stratification of patients, th...

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Autores principales: Mereiter, Stefan, Polom, Karol, Williams, Coralie, Polonia, Antonio, Guergova-Kuras, Mariana, Karlsson, Niclas G., Roviello, Franco, Magalhães, Ana, Reis, Celso A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162870/
https://www.ncbi.nlm.nih.gov/pubmed/30189652
http://dx.doi.org/10.3390/jcm7090256
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author Mereiter, Stefan
Polom, Karol
Williams, Coralie
Polonia, Antonio
Guergova-Kuras, Mariana
Karlsson, Niclas G.
Roviello, Franco
Magalhães, Ana
Reis, Celso A.
author_facet Mereiter, Stefan
Polom, Karol
Williams, Coralie
Polonia, Antonio
Guergova-Kuras, Mariana
Karlsson, Niclas G.
Roviello, Franco
Magalhães, Ana
Reis, Celso A.
author_sort Mereiter, Stefan
collection PubMed
description Microsatellite instability (MSI) is a distinct molecular subtype of gastric cancer. In recent years, the clinical consequences of MSI and the therapeutic opportunities to target this peculiar cancer subtype became evident. However, despite the importance of MSI for the stratification of patients, the time and resources required for diagnosis still present an obstacle. In an attempt to identify a new marker for MSI in gastric cancer, we evaluated the expression of five cancer-associated glycan epitopes in a cohort of 13 MSI and 17 microsatellite stable (MSS) cases. Our analysis revealed a highly significant (p < 0.001) association between the expression of the Thomsen-Friedenreich (TF) antigen and MSI status. Hence, we present here the identification of the first single marker for MSI in gastric cancer, excelling with a specificity of 94% (16/17), sensitivity of 69.2% (9/13), negative predictive value of 80% (16/20), and positive predictive value of 90% (9/10). The TF antigen, detected by simple antibody-based assays, is highly specific for carcinoma being undetectable in gastric healthy and premalignant epithelia. This finding lays the basis for new studies and holds promise in improving the rapid identification of MSI in the clinical setting.
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spelling pubmed-61628702018-10-02 The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer Mereiter, Stefan Polom, Karol Williams, Coralie Polonia, Antonio Guergova-Kuras, Mariana Karlsson, Niclas G. Roviello, Franco Magalhães, Ana Reis, Celso A. J Clin Med Communication Microsatellite instability (MSI) is a distinct molecular subtype of gastric cancer. In recent years, the clinical consequences of MSI and the therapeutic opportunities to target this peculiar cancer subtype became evident. However, despite the importance of MSI for the stratification of patients, the time and resources required for diagnosis still present an obstacle. In an attempt to identify a new marker for MSI in gastric cancer, we evaluated the expression of five cancer-associated glycan epitopes in a cohort of 13 MSI and 17 microsatellite stable (MSS) cases. Our analysis revealed a highly significant (p < 0.001) association between the expression of the Thomsen-Friedenreich (TF) antigen and MSI status. Hence, we present here the identification of the first single marker for MSI in gastric cancer, excelling with a specificity of 94% (16/17), sensitivity of 69.2% (9/13), negative predictive value of 80% (16/20), and positive predictive value of 90% (9/10). The TF antigen, detected by simple antibody-based assays, is highly specific for carcinoma being undetectable in gastric healthy and premalignant epithelia. This finding lays the basis for new studies and holds promise in improving the rapid identification of MSI in the clinical setting. MDPI 2018-09-05 /pmc/articles/PMC6162870/ /pubmed/30189652 http://dx.doi.org/10.3390/jcm7090256 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mereiter, Stefan
Polom, Karol
Williams, Coralie
Polonia, Antonio
Guergova-Kuras, Mariana
Karlsson, Niclas G.
Roviello, Franco
Magalhães, Ana
Reis, Celso A.
The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer
title The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer
title_full The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer
title_fullStr The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer
title_full_unstemmed The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer
title_short The Thomsen-Friedenreich Antigen: A Highly Sensitive and Specific Predictor of Microsatellite Instability in Gastric Cancer
title_sort thomsen-friedenreich antigen: a highly sensitive and specific predictor of microsatellite instability in gastric cancer
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162870/
https://www.ncbi.nlm.nih.gov/pubmed/30189652
http://dx.doi.org/10.3390/jcm7090256
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