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Label-Free Monitoring of Human IgG/Anti-IgG Recognition Using Bloch Surface Waves on 1D Photonic Crystals

Optical biosensors based on one-dimensional photonic crystals sustaining Bloch surface waves are proposed to study antibody interactions and perform affinity studies. The presented approach utilizes two types of different antibodies anchored at the sensitive area of a photonic crystal-based biosenso...

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Detalles Bibliográficos
Autores principales: Sinibaldi, Alberto, Occhicone, Agostino, Munzert, Peter, Danz, Norbert, Sonntag, Frank, Michelotti, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163225/
https://www.ncbi.nlm.nih.gov/pubmed/30044392
http://dx.doi.org/10.3390/bios8030071
Descripción
Sumario:Optical biosensors based on one-dimensional photonic crystals sustaining Bloch surface waves are proposed to study antibody interactions and perform affinity studies. The presented approach utilizes two types of different antibodies anchored at the sensitive area of a photonic crystal-based biosensor. Such a strategy allows for creating two or more on-chip regions with different biochemical features as well as studying the binding kinetics of biomolecules in real time. In particular, the proposed detection system shows an estimated limit of detection for the target antibody (anti-human IgG) smaller than 0.19 nM (28 ng/mL), corresponding to a minimum surface mass coverage of 10.3 ng/cm(2). Moreover, from the binding curves we successfully derived the equilibrium association and dissociation constants (K(A) = 7.5 × 10(7) M(−1); K(D) = 13.26 nM) of the human IgG–anti-human IgG interaction.