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Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration

Background: Current resorbable and non-resorbable membranes act as a physical barrier to avoid connective and epithelial tissue downgrowth into the defect, favoring the regeneration of periodontal tissues. These conventional membranes possess many structural and bio-functional limitations. We hypoth...

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Autores principales: Sunandhakumari, Vishnu Jayakumar, Vidhyadharan, Arun Kumar, Alim, Aneesh, Kumar, Deepan, Ravindran, Jayakrishnan, Krishna, Aswathy, Prasad, Manoj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163249/
https://www.ncbi.nlm.nih.gov/pubmed/30011946
http://dx.doi.org/10.3390/bioengineering5030054
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author Sunandhakumari, Vishnu Jayakumar
Vidhyadharan, Arun Kumar
Alim, Aneesh
Kumar, Deepan
Ravindran, Jayakrishnan
Krishna, Aswathy
Prasad, Manoj
author_facet Sunandhakumari, Vishnu Jayakumar
Vidhyadharan, Arun Kumar
Alim, Aneesh
Kumar, Deepan
Ravindran, Jayakrishnan
Krishna, Aswathy
Prasad, Manoj
author_sort Sunandhakumari, Vishnu Jayakumar
collection PubMed
description Background: Current resorbable and non-resorbable membranes act as a physical barrier to avoid connective and epithelial tissue downgrowth into the defect, favoring the regeneration of periodontal tissues. These conventional membranes possess many structural and bio-functional limitations. We hypothesized that the next-generation of guided tissue regeneration (GTR) membranes for periodontal tissue engineering will be a biologically active, spatially designed nanofibrous biomaterial that closely mimics the native extra-cellular matrix (ECM). Methods: GTR membranes made of poly(ε-Caprolactone) with a molecular weight of 80,000 reinforced with different weight concentrations of nano-Hydroxyapatite/Bioactive glass (2%, 5%, 10%, 15%) is fabricated by the method of electrospinning. After fabrication, in vitro properties are evaluated. Results: The electrospun nanofibrous membranes possessed excellent mechanical properties initially and after one month of degradation in phosphate buffer solution (PBS). Moreover, none of the fabricated membranes were found to be cytotoxic at lower concentrations and higher concentrations. Comparing the overall properties, PCL (poly(e-caprolactone)) + BG (Bioactive glass) 2% exhibited superior cell attachment and percentage of viable cells, increased fiber and pore diameter which satisfies the ideal properties needed for GTR membranes. Conclusion: Composite nanofibrous membranes prepared by electrospinning are suitable for use as a GTR membrane and are a useful prototype for further development of a final membrane for clinical use.
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spelling pubmed-61632492018-10-11 Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration Sunandhakumari, Vishnu Jayakumar Vidhyadharan, Arun Kumar Alim, Aneesh Kumar, Deepan Ravindran, Jayakrishnan Krishna, Aswathy Prasad, Manoj Bioengineering (Basel) Article Background: Current resorbable and non-resorbable membranes act as a physical barrier to avoid connective and epithelial tissue downgrowth into the defect, favoring the regeneration of periodontal tissues. These conventional membranes possess many structural and bio-functional limitations. We hypothesized that the next-generation of guided tissue regeneration (GTR) membranes for periodontal tissue engineering will be a biologically active, spatially designed nanofibrous biomaterial that closely mimics the native extra-cellular matrix (ECM). Methods: GTR membranes made of poly(ε-Caprolactone) with a molecular weight of 80,000 reinforced with different weight concentrations of nano-Hydroxyapatite/Bioactive glass (2%, 5%, 10%, 15%) is fabricated by the method of electrospinning. After fabrication, in vitro properties are evaluated. Results: The electrospun nanofibrous membranes possessed excellent mechanical properties initially and after one month of degradation in phosphate buffer solution (PBS). Moreover, none of the fabricated membranes were found to be cytotoxic at lower concentrations and higher concentrations. Comparing the overall properties, PCL (poly(e-caprolactone)) + BG (Bioactive glass) 2% exhibited superior cell attachment and percentage of viable cells, increased fiber and pore diameter which satisfies the ideal properties needed for GTR membranes. Conclusion: Composite nanofibrous membranes prepared by electrospinning are suitable for use as a GTR membrane and are a useful prototype for further development of a final membrane for clinical use. MDPI 2018-07-15 /pmc/articles/PMC6163249/ /pubmed/30011946 http://dx.doi.org/10.3390/bioengineering5030054 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sunandhakumari, Vishnu Jayakumar
Vidhyadharan, Arun Kumar
Alim, Aneesh
Kumar, Deepan
Ravindran, Jayakrishnan
Krishna, Aswathy
Prasad, Manoj
Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration
title Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration
title_full Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration
title_fullStr Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration
title_full_unstemmed Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration
title_short Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration
title_sort fabrication and in vitro characterization of bioactive glass/nano hydroxyapatite reinforced electrospun poly(ε-caprolactone) composite membranes for guided tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163249/
https://www.ncbi.nlm.nih.gov/pubmed/30011946
http://dx.doi.org/10.3390/bioengineering5030054
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