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Triplex Real-Time PCR without DNA Extraction for the Monitoring of Meningococcal Disease
Detection of Neisseria meningitidis has become less time- and resource-intensive with a monoplex direct real-time PCR (drt-PCR) to amplify genes from clinical specimens without DNA extraction. To further improve efficiency, we evaluated two triplex drt-PCR assays for the detection of meningococcal s...
Autores principales: | Whaley, Melissa J., Jenkins, Laurel T., Hu, Fang, Chen, Alexander, Diarra, Seydou, Ouédraogo-Traoré, Rasmata, Sacchi, Claudio T., Wang, Xin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163423/ https://www.ncbi.nlm.nih.gov/pubmed/30200184 http://dx.doi.org/10.3390/diagnostics8030058 |
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