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Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles
Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. H...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163445/ https://www.ncbi.nlm.nih.gov/pubmed/30201889 http://dx.doi.org/10.3390/nano8090707 |
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author | Marcus, Michal Smith, Alexandra Maswadeh, Ahmad Shemesh, Ziv Zak, Idan Motiei, Menachem Schori, Hadas Margel, Shlomo Sharoni, Amos Shefi, Orit |
author_facet | Marcus, Michal Smith, Alexandra Maswadeh, Ahmad Shemesh, Ziv Zak, Idan Motiei, Menachem Schori, Hadas Margel, Shlomo Sharoni, Amos Shefi, Orit |
author_sort | Marcus, Michal |
collection | PubMed |
description | Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF–MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic “hot spots”. To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics. |
format | Online Article Text |
id | pubmed-6163445 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61634452018-10-10 Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles Marcus, Michal Smith, Alexandra Maswadeh, Ahmad Shemesh, Ziv Zak, Idan Motiei, Menachem Schori, Hadas Margel, Shlomo Sharoni, Amos Shefi, Orit Nanomaterials (Basel) Article Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF–MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic “hot spots”. To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics. MDPI 2018-09-10 /pmc/articles/PMC6163445/ /pubmed/30201889 http://dx.doi.org/10.3390/nano8090707 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marcus, Michal Smith, Alexandra Maswadeh, Ahmad Shemesh, Ziv Zak, Idan Motiei, Menachem Schori, Hadas Margel, Shlomo Sharoni, Amos Shefi, Orit Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
title | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
title_full | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
title_fullStr | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
title_full_unstemmed | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
title_short | Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles |
title_sort | magnetic targeting of growth factors using iron oxide nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163445/ https://www.ncbi.nlm.nih.gov/pubmed/30201889 http://dx.doi.org/10.3390/nano8090707 |
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