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Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi

Many marine bacteria secrete exopolysaccharides (EPSs), which are made up of a substantial component of the macro-molecules surrounding cells. Recently, the wide demand for EPSs for food, cosmetics, pharmaceutical and other applications has led to great interest in them. In this study, an EPS produc...

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Autores principales: Wang, Chunlei, Fan, Qiuping, Zhang, Xiaofei, Lu, Xiaoping, Xu, Yanrui, Zhu, Wenxing, Zhang, Jie, Hao, Wen, Hao, Lujiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163479/
https://www.ncbi.nlm.nih.gov/pubmed/30223612
http://dx.doi.org/10.3390/md16090337
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author Wang, Chunlei
Fan, Qiuping
Zhang, Xiaofei
Lu, Xiaoping
Xu, Yanrui
Zhu, Wenxing
Zhang, Jie
Hao, Wen
Hao, Lujiang
author_facet Wang, Chunlei
Fan, Qiuping
Zhang, Xiaofei
Lu, Xiaoping
Xu, Yanrui
Zhu, Wenxing
Zhang, Jie
Hao, Wen
Hao, Lujiang
author_sort Wang, Chunlei
collection PubMed
description Many marine bacteria secrete exopolysaccharides (EPSs), which are made up of a substantial component of the macro-molecules surrounding cells. Recently, the wide demand for EPSs for food, cosmetics, pharmaceutical and other applications has led to great interest in them. In this study, an EPS produced by marine bacteria Aerococcus uriaeequi HZ strains (EPS-A) was isolated and purified to examine its structure and biological function. The molecular weight of EPS-A analyzed by high-performance liquid gel filtration chromatography (HPGFC) is found to have a number average of 2.22 × 10(5) and weight average of 2.84 × 10(5), respectively. High-performance liquid chromatography (HPLC) and Fourier-transform–infrared (FT–IR) analysis indicate that EPS-A was a polysaccharide composed of glucose and a little mannose. In addition, the flocculating rate of sewage of EPS-A was 79.90%. The hygroscopicity studies showed that hygroscopicity of EPS-A was higher than chitosan but lower than that of sodium hyaluronate. The moisture retention of EPS-A showed similar retention activity to both chitosan and sodium hyaluronate. EPS-A also can scavenge free radicals including both OH• free radical and O(2)•(−) free radical and the activity to O(2)•(−) free radical is similar to vitamin C. Safety assessment on mice indicated that the EPS-A is safe for external use and oral administration. EPS-A has great potential for applications in medicine due to its characteristics mentioned above.
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spelling pubmed-61634792018-10-11 Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi Wang, Chunlei Fan, Qiuping Zhang, Xiaofei Lu, Xiaoping Xu, Yanrui Zhu, Wenxing Zhang, Jie Hao, Wen Hao, Lujiang Mar Drugs Article Many marine bacteria secrete exopolysaccharides (EPSs), which are made up of a substantial component of the macro-molecules surrounding cells. Recently, the wide demand for EPSs for food, cosmetics, pharmaceutical and other applications has led to great interest in them. In this study, an EPS produced by marine bacteria Aerococcus uriaeequi HZ strains (EPS-A) was isolated and purified to examine its structure and biological function. The molecular weight of EPS-A analyzed by high-performance liquid gel filtration chromatography (HPGFC) is found to have a number average of 2.22 × 10(5) and weight average of 2.84 × 10(5), respectively. High-performance liquid chromatography (HPLC) and Fourier-transform–infrared (FT–IR) analysis indicate that EPS-A was a polysaccharide composed of glucose and a little mannose. In addition, the flocculating rate of sewage of EPS-A was 79.90%. The hygroscopicity studies showed that hygroscopicity of EPS-A was higher than chitosan but lower than that of sodium hyaluronate. The moisture retention of EPS-A showed similar retention activity to both chitosan and sodium hyaluronate. EPS-A also can scavenge free radicals including both OH• free radical and O(2)•(−) free radical and the activity to O(2)•(−) free radical is similar to vitamin C. Safety assessment on mice indicated that the EPS-A is safe for external use and oral administration. EPS-A has great potential for applications in medicine due to its characteristics mentioned above. MDPI 2018-09-16 /pmc/articles/PMC6163479/ /pubmed/30223612 http://dx.doi.org/10.3390/md16090337 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Chunlei
Fan, Qiuping
Zhang, Xiaofei
Lu, Xiaoping
Xu, Yanrui
Zhu, Wenxing
Zhang, Jie
Hao, Wen
Hao, Lujiang
Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
title Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
title_full Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
title_fullStr Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
title_full_unstemmed Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
title_short Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
title_sort isolation, characterization, and pharmaceutical applications of an exopolysaccharide from aerococcus uriaeequi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163479/
https://www.ncbi.nlm.nih.gov/pubmed/30223612
http://dx.doi.org/10.3390/md16090337
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