Cargando…
Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi
Many marine bacteria secrete exopolysaccharides (EPSs), which are made up of a substantial component of the macro-molecules surrounding cells. Recently, the wide demand for EPSs for food, cosmetics, pharmaceutical and other applications has led to great interest in them. In this study, an EPS produc...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163479/ https://www.ncbi.nlm.nih.gov/pubmed/30223612 http://dx.doi.org/10.3390/md16090337 |
_version_ | 1783359371054415872 |
---|---|
author | Wang, Chunlei Fan, Qiuping Zhang, Xiaofei Lu, Xiaoping Xu, Yanrui Zhu, Wenxing Zhang, Jie Hao, Wen Hao, Lujiang |
author_facet | Wang, Chunlei Fan, Qiuping Zhang, Xiaofei Lu, Xiaoping Xu, Yanrui Zhu, Wenxing Zhang, Jie Hao, Wen Hao, Lujiang |
author_sort | Wang, Chunlei |
collection | PubMed |
description | Many marine bacteria secrete exopolysaccharides (EPSs), which are made up of a substantial component of the macro-molecules surrounding cells. Recently, the wide demand for EPSs for food, cosmetics, pharmaceutical and other applications has led to great interest in them. In this study, an EPS produced by marine bacteria Aerococcus uriaeequi HZ strains (EPS-A) was isolated and purified to examine its structure and biological function. The molecular weight of EPS-A analyzed by high-performance liquid gel filtration chromatography (HPGFC) is found to have a number average of 2.22 × 10(5) and weight average of 2.84 × 10(5), respectively. High-performance liquid chromatography (HPLC) and Fourier-transform–infrared (FT–IR) analysis indicate that EPS-A was a polysaccharide composed of glucose and a little mannose. In addition, the flocculating rate of sewage of EPS-A was 79.90%. The hygroscopicity studies showed that hygroscopicity of EPS-A was higher than chitosan but lower than that of sodium hyaluronate. The moisture retention of EPS-A showed similar retention activity to both chitosan and sodium hyaluronate. EPS-A also can scavenge free radicals including both OH• free radical and O(2)•(−) free radical and the activity to O(2)•(−) free radical is similar to vitamin C. Safety assessment on mice indicated that the EPS-A is safe for external use and oral administration. EPS-A has great potential for applications in medicine due to its characteristics mentioned above. |
format | Online Article Text |
id | pubmed-6163479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61634792018-10-11 Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi Wang, Chunlei Fan, Qiuping Zhang, Xiaofei Lu, Xiaoping Xu, Yanrui Zhu, Wenxing Zhang, Jie Hao, Wen Hao, Lujiang Mar Drugs Article Many marine bacteria secrete exopolysaccharides (EPSs), which are made up of a substantial component of the macro-molecules surrounding cells. Recently, the wide demand for EPSs for food, cosmetics, pharmaceutical and other applications has led to great interest in them. In this study, an EPS produced by marine bacteria Aerococcus uriaeequi HZ strains (EPS-A) was isolated and purified to examine its structure and biological function. The molecular weight of EPS-A analyzed by high-performance liquid gel filtration chromatography (HPGFC) is found to have a number average of 2.22 × 10(5) and weight average of 2.84 × 10(5), respectively. High-performance liquid chromatography (HPLC) and Fourier-transform–infrared (FT–IR) analysis indicate that EPS-A was a polysaccharide composed of glucose and a little mannose. In addition, the flocculating rate of sewage of EPS-A was 79.90%. The hygroscopicity studies showed that hygroscopicity of EPS-A was higher than chitosan but lower than that of sodium hyaluronate. The moisture retention of EPS-A showed similar retention activity to both chitosan and sodium hyaluronate. EPS-A also can scavenge free radicals including both OH• free radical and O(2)•(−) free radical and the activity to O(2)•(−) free radical is similar to vitamin C. Safety assessment on mice indicated that the EPS-A is safe for external use and oral administration. EPS-A has great potential for applications in medicine due to its characteristics mentioned above. MDPI 2018-09-16 /pmc/articles/PMC6163479/ /pubmed/30223612 http://dx.doi.org/10.3390/md16090337 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Chunlei Fan, Qiuping Zhang, Xiaofei Lu, Xiaoping Xu, Yanrui Zhu, Wenxing Zhang, Jie Hao, Wen Hao, Lujiang Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi |
title | Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi |
title_full | Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi |
title_fullStr | Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi |
title_full_unstemmed | Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi |
title_short | Isolation, Characterization, and Pharmaceutical Applications of an Exopolysaccharide from Aerococcus Uriaeequi |
title_sort | isolation, characterization, and pharmaceutical applications of an exopolysaccharide from aerococcus uriaeequi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163479/ https://www.ncbi.nlm.nih.gov/pubmed/30223612 http://dx.doi.org/10.3390/md16090337 |
work_keys_str_mv | AT wangchunlei isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT fanqiuping isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT zhangxiaofei isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT luxiaoping isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT xuyanrui isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT zhuwenxing isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT zhangjie isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT haowen isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi AT haolujiang isolationcharacterizationandpharmaceuticalapplicationsofanexopolysaccharidefromaerococcusuriaeequi |