Cargando…
Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis
RAB5 is a small GTPase that belongs to the wide family of Rab proteins and localizes on early endosomes. In its active GTP-bound form, RAB5 recruits downstream effectors that, in turn, are responsible for distinct aspects of early endosome function, including their movement along microtubules. We pr...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163544/ https://www.ncbi.nlm.nih.gov/pubmed/30200238 http://dx.doi.org/10.3390/ijms19092575 |
_version_ | 1783359386622623744 |
---|---|
author | Pupo, Emanuela Avanzato, Daniele Scianna, Marco Oldani, Amanda Serini, Guido Lanzetti, Letizia |
author_facet | Pupo, Emanuela Avanzato, Daniele Scianna, Marco Oldani, Amanda Serini, Guido Lanzetti, Letizia |
author_sort | Pupo, Emanuela |
collection | PubMed |
description | RAB5 is a small GTPase that belongs to the wide family of Rab proteins and localizes on early endosomes. In its active GTP-bound form, RAB5 recruits downstream effectors that, in turn, are responsible for distinct aspects of early endosome function, including their movement along microtubules. We previously reported that, at the onset of mitosis, RAB5positive vesicles cluster around the spindle poles and, during metaphase, move along spindle microtubules. RNAi-mediated depletion of the three RAB5 isoforms delays nuclear envelope breakdown at prophase and severely affects chromosome alignment and segregation. Here we show that depletion of the Kinesin-2 motor complex impairs long-range movement of RAB5 endosomes in interphase cells and prevents localization of these vesicles at the spindle during metaphase. Similarly to the effect caused by RAB5 depletion, functional ablation of Kinesin-2 delays nuclear envelope breakdown resulting in prolonged prophase. Altogether these findings suggest that endosomal transport at the onset of mitosis is required to control timing of nuclear envelope breakdown. |
format | Online Article Text |
id | pubmed-6163544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61635442018-10-10 Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis Pupo, Emanuela Avanzato, Daniele Scianna, Marco Oldani, Amanda Serini, Guido Lanzetti, Letizia Int J Mol Sci Article RAB5 is a small GTPase that belongs to the wide family of Rab proteins and localizes on early endosomes. In its active GTP-bound form, RAB5 recruits downstream effectors that, in turn, are responsible for distinct aspects of early endosome function, including their movement along microtubules. We previously reported that, at the onset of mitosis, RAB5positive vesicles cluster around the spindle poles and, during metaphase, move along spindle microtubules. RNAi-mediated depletion of the three RAB5 isoforms delays nuclear envelope breakdown at prophase and severely affects chromosome alignment and segregation. Here we show that depletion of the Kinesin-2 motor complex impairs long-range movement of RAB5 endosomes in interphase cells and prevents localization of these vesicles at the spindle during metaphase. Similarly to the effect caused by RAB5 depletion, functional ablation of Kinesin-2 delays nuclear envelope breakdown resulting in prolonged prophase. Altogether these findings suggest that endosomal transport at the onset of mitosis is required to control timing of nuclear envelope breakdown. MDPI 2018-08-30 /pmc/articles/PMC6163544/ /pubmed/30200238 http://dx.doi.org/10.3390/ijms19092575 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pupo, Emanuela Avanzato, Daniele Scianna, Marco Oldani, Amanda Serini, Guido Lanzetti, Letizia Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis |
title | Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis |
title_full | Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis |
title_fullStr | Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis |
title_full_unstemmed | Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis |
title_short | Kinesin-2 Controls the Motility of RAB5 Endosomes and Their Association with the Spindle in Mitosis |
title_sort | kinesin-2 controls the motility of rab5 endosomes and their association with the spindle in mitosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163544/ https://www.ncbi.nlm.nih.gov/pubmed/30200238 http://dx.doi.org/10.3390/ijms19092575 |
work_keys_str_mv | AT pupoemanuela kinesin2controlsthemotilityofrab5endosomesandtheirassociationwiththespindleinmitosis AT avanzatodaniele kinesin2controlsthemotilityofrab5endosomesandtheirassociationwiththespindleinmitosis AT sciannamarco kinesin2controlsthemotilityofrab5endosomesandtheirassociationwiththespindleinmitosis AT oldaniamanda kinesin2controlsthemotilityofrab5endosomesandtheirassociationwiththespindleinmitosis AT seriniguido kinesin2controlsthemotilityofrab5endosomesandtheirassociationwiththespindleinmitosis AT lanzettiletizia kinesin2controlsthemotilityofrab5endosomesandtheirassociationwiththespindleinmitosis |