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Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System
Recently, the benefit of step-wise sequential delivery of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 from a bioinspired apatite drug delivery system on mouse calvarial bone repair was demonstrated. The thicknesses of the nanostructured poly-l-Lysine/poly-l-Glutamic acid poly...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163578/ https://www.ncbi.nlm.nih.gov/pubmed/30217000 http://dx.doi.org/10.3390/ma11091703 |
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author | Alhamdi, Jumana Jacobs, Emily Gronowicz, Gloria Benkirane-Jessel, Nadia Hurley, Marja Kuhn, Liisa |
author_facet | Alhamdi, Jumana Jacobs, Emily Gronowicz, Gloria Benkirane-Jessel, Nadia Hurley, Marja Kuhn, Liisa |
author_sort | Alhamdi, Jumana |
collection | PubMed |
description | Recently, the benefit of step-wise sequential delivery of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 from a bioinspired apatite drug delivery system on mouse calvarial bone repair was demonstrated. The thicknesses of the nanostructured poly-l-Lysine/poly-l-Glutamic acid polyelectrolyte multilayer (PEM) and the bone-like apatite barrier layer that make up the delivery system, were varied. The effects of the structural variations of the coating on the kinetics of cell access to a cytotoxic factor delivered by the layered structure were evaluated. FGF-2 was adsorbed into the outer PEM, and cytotoxic antimycin-A (AntiA) was adsorbed to the substrate below the barrier layer to detect the timing of the cell access. While MC3T3-E1 osteoprogenitor cells accessed AntiA after three days, the RAW 264.7 macrophage access occurred within 4 h, unless the PEM layer was removed, in which case the results were reversed. Pits were created in the coating by the RAW 264.7 macrophages and initiated delivery, while the osteoprogenitor cell access to drugs occurred through a solution-mediated coating dissolution, at junctions between the islands of crystals. Macrophage-mediated degradation is therefore a mechanism that controls drug release from coatings containing bioinspired apatite. |
format | Online Article Text |
id | pubmed-6163578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61635782018-10-12 Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System Alhamdi, Jumana Jacobs, Emily Gronowicz, Gloria Benkirane-Jessel, Nadia Hurley, Marja Kuhn, Liisa Materials (Basel) Article Recently, the benefit of step-wise sequential delivery of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 from a bioinspired apatite drug delivery system on mouse calvarial bone repair was demonstrated. The thicknesses of the nanostructured poly-l-Lysine/poly-l-Glutamic acid polyelectrolyte multilayer (PEM) and the bone-like apatite barrier layer that make up the delivery system, were varied. The effects of the structural variations of the coating on the kinetics of cell access to a cytotoxic factor delivered by the layered structure were evaluated. FGF-2 was adsorbed into the outer PEM, and cytotoxic antimycin-A (AntiA) was adsorbed to the substrate below the barrier layer to detect the timing of the cell access. While MC3T3-E1 osteoprogenitor cells accessed AntiA after three days, the RAW 264.7 macrophage access occurred within 4 h, unless the PEM layer was removed, in which case the results were reversed. Pits were created in the coating by the RAW 264.7 macrophages and initiated delivery, while the osteoprogenitor cell access to drugs occurred through a solution-mediated coating dissolution, at junctions between the islands of crystals. Macrophage-mediated degradation is therefore a mechanism that controls drug release from coatings containing bioinspired apatite. MDPI 2018-09-13 /pmc/articles/PMC6163578/ /pubmed/30217000 http://dx.doi.org/10.3390/ma11091703 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alhamdi, Jumana Jacobs, Emily Gronowicz, Gloria Benkirane-Jessel, Nadia Hurley, Marja Kuhn, Liisa Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System |
title | Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System |
title_full | Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System |
title_fullStr | Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System |
title_full_unstemmed | Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System |
title_short | Cell Type Influences Local Delivery of Biomolecules from a Bioinspired Apatite Drug Delivery System |
title_sort | cell type influences local delivery of biomolecules from a bioinspired apatite drug delivery system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163578/ https://www.ncbi.nlm.nih.gov/pubmed/30217000 http://dx.doi.org/10.3390/ma11091703 |
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