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Cytokeratins Biosensing Using Tilted Fiber Gratings
Optical fiber gratings have widely proven their applicability in biosensing, especially when they are coupled with antibodies for specific antigen recognition. While this is customarily done with fibers coated by a thin metal film to benefit from plasmonic enhancement, in this paper, we propose to s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163579/ https://www.ncbi.nlm.nih.gov/pubmed/30081506 http://dx.doi.org/10.3390/bios8030074 |
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author | Loyez, Médéric Albert, Jacques Caucheteur, Christophe Wattiez, Ruddy |
author_facet | Loyez, Médéric Albert, Jacques Caucheteur, Christophe Wattiez, Ruddy |
author_sort | Loyez, Médéric |
collection | PubMed |
description | Optical fiber gratings have widely proven their applicability in biosensing, especially when they are coupled with antibodies for specific antigen recognition. While this is customarily done with fibers coated by a thin metal film to benefit from plasmonic enhancement, in this paper, we propose to study their intrinsic properties, developing a label-free sensor for the detection of biomarkers in real-time without metal coatings for surface plasmon resonances. We focus on the inner properties of our modal sensor by immobilizing receptors directly on the silica surface, and reporting the sensitivity of bare tilted fiber Bragg gratings (TFBGs) used at near infrared wavelengths. We test different strategies to build our sensing surface against cytokeratins and show that the most reliable functionalization method is the electrostatic adsorption of antibodies on the fiber, allowing a limit of detection reaching 14 pM by following the guided cladding modes near the cut-off area. These results present the biodetection performance that TFBGs bring through their modal properties for different functionalizations and data processing strategies. |
format | Online Article Text |
id | pubmed-6163579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61635792018-10-10 Cytokeratins Biosensing Using Tilted Fiber Gratings Loyez, Médéric Albert, Jacques Caucheteur, Christophe Wattiez, Ruddy Biosensors (Basel) Communication Optical fiber gratings have widely proven their applicability in biosensing, especially when they are coupled with antibodies for specific antigen recognition. While this is customarily done with fibers coated by a thin metal film to benefit from plasmonic enhancement, in this paper, we propose to study their intrinsic properties, developing a label-free sensor for the detection of biomarkers in real-time without metal coatings for surface plasmon resonances. We focus on the inner properties of our modal sensor by immobilizing receptors directly on the silica surface, and reporting the sensitivity of bare tilted fiber Bragg gratings (TFBGs) used at near infrared wavelengths. We test different strategies to build our sensing surface against cytokeratins and show that the most reliable functionalization method is the electrostatic adsorption of antibodies on the fiber, allowing a limit of detection reaching 14 pM by following the guided cladding modes near the cut-off area. These results present the biodetection performance that TFBGs bring through their modal properties for different functionalizations and data processing strategies. MDPI 2018-08-03 /pmc/articles/PMC6163579/ /pubmed/30081506 http://dx.doi.org/10.3390/bios8030074 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Loyez, Médéric Albert, Jacques Caucheteur, Christophe Wattiez, Ruddy Cytokeratins Biosensing Using Tilted Fiber Gratings |
title | Cytokeratins Biosensing Using Tilted Fiber Gratings |
title_full | Cytokeratins Biosensing Using Tilted Fiber Gratings |
title_fullStr | Cytokeratins Biosensing Using Tilted Fiber Gratings |
title_full_unstemmed | Cytokeratins Biosensing Using Tilted Fiber Gratings |
title_short | Cytokeratins Biosensing Using Tilted Fiber Gratings |
title_sort | cytokeratins biosensing using tilted fiber gratings |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163579/ https://www.ncbi.nlm.nih.gov/pubmed/30081506 http://dx.doi.org/10.3390/bios8030074 |
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