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Cytokeratins Biosensing Using Tilted Fiber Gratings

Optical fiber gratings have widely proven their applicability in biosensing, especially when they are coupled with antibodies for specific antigen recognition. While this is customarily done with fibers coated by a thin metal film to benefit from plasmonic enhancement, in this paper, we propose to s...

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Detalles Bibliográficos
Autores principales: Loyez, Médéric, Albert, Jacques, Caucheteur, Christophe, Wattiez, Ruddy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163579/
https://www.ncbi.nlm.nih.gov/pubmed/30081506
http://dx.doi.org/10.3390/bios8030074
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author Loyez, Médéric
Albert, Jacques
Caucheteur, Christophe
Wattiez, Ruddy
author_facet Loyez, Médéric
Albert, Jacques
Caucheteur, Christophe
Wattiez, Ruddy
author_sort Loyez, Médéric
collection PubMed
description Optical fiber gratings have widely proven their applicability in biosensing, especially when they are coupled with antibodies for specific antigen recognition. While this is customarily done with fibers coated by a thin metal film to benefit from plasmonic enhancement, in this paper, we propose to study their intrinsic properties, developing a label-free sensor for the detection of biomarkers in real-time without metal coatings for surface plasmon resonances. We focus on the inner properties of our modal sensor by immobilizing receptors directly on the silica surface, and reporting the sensitivity of bare tilted fiber Bragg gratings (TFBGs) used at near infrared wavelengths. We test different strategies to build our sensing surface against cytokeratins and show that the most reliable functionalization method is the electrostatic adsorption of antibodies on the fiber, allowing a limit of detection reaching 14 pM by following the guided cladding modes near the cut-off area. These results present the biodetection performance that TFBGs bring through their modal properties for different functionalizations and data processing strategies.
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spelling pubmed-61635792018-10-10 Cytokeratins Biosensing Using Tilted Fiber Gratings Loyez, Médéric Albert, Jacques Caucheteur, Christophe Wattiez, Ruddy Biosensors (Basel) Communication Optical fiber gratings have widely proven their applicability in biosensing, especially when they are coupled with antibodies for specific antigen recognition. While this is customarily done with fibers coated by a thin metal film to benefit from plasmonic enhancement, in this paper, we propose to study their intrinsic properties, developing a label-free sensor for the detection of biomarkers in real-time without metal coatings for surface plasmon resonances. We focus on the inner properties of our modal sensor by immobilizing receptors directly on the silica surface, and reporting the sensitivity of bare tilted fiber Bragg gratings (TFBGs) used at near infrared wavelengths. We test different strategies to build our sensing surface against cytokeratins and show that the most reliable functionalization method is the electrostatic adsorption of antibodies on the fiber, allowing a limit of detection reaching 14 pM by following the guided cladding modes near the cut-off area. These results present the biodetection performance that TFBGs bring through their modal properties for different functionalizations and data processing strategies. MDPI 2018-08-03 /pmc/articles/PMC6163579/ /pubmed/30081506 http://dx.doi.org/10.3390/bios8030074 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Loyez, Médéric
Albert, Jacques
Caucheteur, Christophe
Wattiez, Ruddy
Cytokeratins Biosensing Using Tilted Fiber Gratings
title Cytokeratins Biosensing Using Tilted Fiber Gratings
title_full Cytokeratins Biosensing Using Tilted Fiber Gratings
title_fullStr Cytokeratins Biosensing Using Tilted Fiber Gratings
title_full_unstemmed Cytokeratins Biosensing Using Tilted Fiber Gratings
title_short Cytokeratins Biosensing Using Tilted Fiber Gratings
title_sort cytokeratins biosensing using tilted fiber gratings
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163579/
https://www.ncbi.nlm.nih.gov/pubmed/30081506
http://dx.doi.org/10.3390/bios8030074
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AT wattiezruddy cytokeratinsbiosensingusingtiltedfibergratings