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PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway
Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress response...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163601/ https://www.ncbi.nlm.nih.gov/pubmed/30177627 http://dx.doi.org/10.3390/ijms19092609 |
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author | Li, Ping Yang, Hong Liu, Gaojing Ma, Wenzhang Li, Chuanhong Huo, Heqiang He, Jianfang Liu, Li |
author_facet | Li, Ping Yang, Hong Liu, Gaojing Ma, Wenzhang Li, Chuanhong Huo, Heqiang He, Jianfang Liu, Li |
author_sort | Li, Ping |
collection | PubMed |
description | Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress responses in moss are not well known. Here, we investigated functional roles of SARK isolated from Physcomitrella patens (PpSARK) in salt stress response and senescence. PpSARK transcripts significantly accumulated under NaCl and abscisic acid (ABA) treatments, with higher expression in the moss gametophyte stage. Insertional gain-of-function mutants of PpSARK (PpSARKg) were more tolerant to salt stress and ABA than wild type (WT), whereas senescence of mutants was delayed during the protonema stage. Expression of stress-responsive genes in the ABA related pathway, such as PpABI3, PpABI5, PpPP2C, and PpLEA were significantly higher in PpSARKg and WT under salt stress conditions, suggesting that PpSARK might positively regulate salt tolerance via an ABA-related pathway. Endogenous ABA contents also increased 3-fold under salt stress conditions. These results indicate that PpSARK functions as a positive regulator in salt stress responses, while possibly functioning as a negative regulator in senescence in moss. |
format | Online Article Text |
id | pubmed-6163601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61636012018-10-10 PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway Li, Ping Yang, Hong Liu, Gaojing Ma, Wenzhang Li, Chuanhong Huo, Heqiang He, Jianfang Liu, Li Int J Mol Sci Article Senescence-associated receptor-like kinase (SARK) family members in Arabidopsis, soybean, and rice are known to be positive regulators of leaf senescence. In the meantime, SARKs are extensively involved in stress response. However, their function and underlying molecular mechanism in stress responses in moss are not well known. Here, we investigated functional roles of SARK isolated from Physcomitrella patens (PpSARK) in salt stress response and senescence. PpSARK transcripts significantly accumulated under NaCl and abscisic acid (ABA) treatments, with higher expression in the moss gametophyte stage. Insertional gain-of-function mutants of PpSARK (PpSARKg) were more tolerant to salt stress and ABA than wild type (WT), whereas senescence of mutants was delayed during the protonema stage. Expression of stress-responsive genes in the ABA related pathway, such as PpABI3, PpABI5, PpPP2C, and PpLEA were significantly higher in PpSARKg and WT under salt stress conditions, suggesting that PpSARK might positively regulate salt tolerance via an ABA-related pathway. Endogenous ABA contents also increased 3-fold under salt stress conditions. These results indicate that PpSARK functions as a positive regulator in salt stress responses, while possibly functioning as a negative regulator in senescence in moss. MDPI 2018-09-03 /pmc/articles/PMC6163601/ /pubmed/30177627 http://dx.doi.org/10.3390/ijms19092609 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Ping Yang, Hong Liu, Gaojing Ma, Wenzhang Li, Chuanhong Huo, Heqiang He, Jianfang Liu, Li PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title | PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_full | PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_fullStr | PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_full_unstemmed | PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_short | PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway |
title_sort | ppsark regulates moss senescence and salt tolerance through aba related pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163601/ https://www.ncbi.nlm.nih.gov/pubmed/30177627 http://dx.doi.org/10.3390/ijms19092609 |
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