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Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infecti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163637/ https://www.ncbi.nlm.nih.gov/pubmed/30150524 http://dx.doi.org/10.3390/ma11091541 |
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author | Savas, Sumeyra Ersoy, Aylin Gulmez, Yakup Kilic, Selcuk Levent, Belkis Altintas, Zeynep |
author_facet | Savas, Sumeyra Ersoy, Aylin Gulmez, Yakup Kilic, Selcuk Levent, Belkis Altintas, Zeynep |
author_sort | Savas, Sumeyra |
collection | PubMed |
description | Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL(−1). The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection. |
format | Online Article Text |
id | pubmed-6163637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61636372018-10-12 Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella Savas, Sumeyra Ersoy, Aylin Gulmez, Yakup Kilic, Selcuk Levent, Belkis Altintas, Zeynep Materials (Basel) Article Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL(−1). The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection. MDPI 2018-08-27 /pmc/articles/PMC6163637/ /pubmed/30150524 http://dx.doi.org/10.3390/ma11091541 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Savas, Sumeyra Ersoy, Aylin Gulmez, Yakup Kilic, Selcuk Levent, Belkis Altintas, Zeynep Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella |
title | Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella |
title_full | Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella |
title_fullStr | Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella |
title_full_unstemmed | Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella |
title_short | Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella |
title_sort | nanoparticle enhanced antibody and dna biosensors for sensitive detection of salmonella |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163637/ https://www.ncbi.nlm.nih.gov/pubmed/30150524 http://dx.doi.org/10.3390/ma11091541 |
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