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Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella

Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infecti...

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Autores principales: Savas, Sumeyra, Ersoy, Aylin, Gulmez, Yakup, Kilic, Selcuk, Levent, Belkis, Altintas, Zeynep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163637/
https://www.ncbi.nlm.nih.gov/pubmed/30150524
http://dx.doi.org/10.3390/ma11091541
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author Savas, Sumeyra
Ersoy, Aylin
Gulmez, Yakup
Kilic, Selcuk
Levent, Belkis
Altintas, Zeynep
author_facet Savas, Sumeyra
Ersoy, Aylin
Gulmez, Yakup
Kilic, Selcuk
Levent, Belkis
Altintas, Zeynep
author_sort Savas, Sumeyra
collection PubMed
description Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL(−1). The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection.
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spelling pubmed-61636372018-10-12 Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella Savas, Sumeyra Ersoy, Aylin Gulmez, Yakup Kilic, Selcuk Levent, Belkis Altintas, Zeynep Materials (Basel) Article Bacteria-related pathogenic diseases are one of the major health problems throughout the world. Salmonella is a genus of rod-shaped Gram-negative enterobacteria of which more than 2600 serotypes have been identified. Infection with Salmonella can cause salmonellosis, a serious bacterial toxi-infection syndrome associated with gastroenteritis, and paralyphoid and typhoid fevers. Its rapid and sensitive detection is a key to the prevention of problems related to health. This paper describes the development of antibody and DNA sensors for Salmonella detection using a microfluidic-based electrochemical system. Commercial Salmonella typhimurium and Salmonella typhimurium from human stool samples were investigated using standard and nanomaterial-amplified antibody sensors. S. typhimurium could be detected down to 1 cfu mL(−1). The specificity of immunoassay was tested by studying with non-specific bacteria including E. coli and S. aureus that revealed only 2.01% and 2.66% binding when compared to the target bacterium. On the other hand, the quantification of Salmonella DNA was investigated in a concentration range of 0.002–200 µM using the developed DNA biosensor that demonstrated very high specificity and sensitivity with a detection limit of 0.94 nM. Our custom-designed microfluidic sensor offers rapid, highly sensitive, and specific diagnostic assay approaches for pathogen detection. MDPI 2018-08-27 /pmc/articles/PMC6163637/ /pubmed/30150524 http://dx.doi.org/10.3390/ma11091541 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Savas, Sumeyra
Ersoy, Aylin
Gulmez, Yakup
Kilic, Selcuk
Levent, Belkis
Altintas, Zeynep
Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
title Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
title_full Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
title_fullStr Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
title_full_unstemmed Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
title_short Nanoparticle Enhanced Antibody and DNA Biosensors for Sensitive Detection of Salmonella
title_sort nanoparticle enhanced antibody and dna biosensors for sensitive detection of salmonella
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163637/
https://www.ncbi.nlm.nih.gov/pubmed/30150524
http://dx.doi.org/10.3390/ma11091541
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