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Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment
Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163784/ https://www.ncbi.nlm.nih.gov/pubmed/30154348 http://dx.doi.org/10.3390/ijms19092549 |
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author | Brandt, Luisa Schubert, Susanna Scheibe, Patrick Brehm, Walter Franzen, Jan Gross, Claudia Burk, Janina |
author_facet | Brandt, Luisa Schubert, Susanna Scheibe, Patrick Brehm, Walter Franzen, Jan Gross, Claudia Burk, Janina |
author_sort | Brandt, Luisa |
collection | PubMed |
description | Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represented by supplementation with interleukin-1β and/or tumor necrosis factor-α or by co-culture with allogeneic peripheral blood leukocytes, on ASC functional properties was investigated. High cytokine concentrations increased ASC proliferation and osteogenic differentiation, but decreased chondrogenic differentiation and ASC viability in scaffold culture, as well as tendon scaffold repopulation, and strongly influenced musculoskeletal gene expression. Effects regarding the latter differed between the monolayer and scaffold cultures. Leukocytes rather decreased ASC proliferation, but had similar effects on viability and musculoskeletal gene expression. This included decreased expression of the tenogenic transcription factor scleraxis by an inflammatory environment throughout culture conditions. The data demonstrate that ASC tenogenic properties are compromised in an inflammatory environment, with relevance to their possible mechanisms of action in acute tendon disease. |
format | Online Article Text |
id | pubmed-6163784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61637842018-10-10 Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment Brandt, Luisa Schubert, Susanna Scheibe, Patrick Brehm, Walter Franzen, Jan Gross, Claudia Burk, Janina Int J Mol Sci Article Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represented by supplementation with interleukin-1β and/or tumor necrosis factor-α or by co-culture with allogeneic peripheral blood leukocytes, on ASC functional properties was investigated. High cytokine concentrations increased ASC proliferation and osteogenic differentiation, but decreased chondrogenic differentiation and ASC viability in scaffold culture, as well as tendon scaffold repopulation, and strongly influenced musculoskeletal gene expression. Effects regarding the latter differed between the monolayer and scaffold cultures. Leukocytes rather decreased ASC proliferation, but had similar effects on viability and musculoskeletal gene expression. This included decreased expression of the tenogenic transcription factor scleraxis by an inflammatory environment throughout culture conditions. The data demonstrate that ASC tenogenic properties are compromised in an inflammatory environment, with relevance to their possible mechanisms of action in acute tendon disease. MDPI 2018-08-28 /pmc/articles/PMC6163784/ /pubmed/30154348 http://dx.doi.org/10.3390/ijms19092549 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Brandt, Luisa Schubert, Susanna Scheibe, Patrick Brehm, Walter Franzen, Jan Gross, Claudia Burk, Janina Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment |
title | Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment |
title_full | Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment |
title_fullStr | Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment |
title_full_unstemmed | Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment |
title_short | Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment |
title_sort | tenogenic properties of mesenchymal progenitor cells are compromised in an inflammatory environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163784/ https://www.ncbi.nlm.nih.gov/pubmed/30154348 http://dx.doi.org/10.3390/ijms19092549 |
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