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A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling
Human milk composition is dynamic, and substitute formulae are intended to mimic its protein content. The purpose of this study was to investigate the potentiality of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), followed by multivariate data analyses a...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163840/ https://www.ncbi.nlm.nih.gov/pubmed/30189627 http://dx.doi.org/10.3390/nu10091238 |
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author | Di Francesco, Laura Di Girolamo, Francesco Mennini, Maurizio Masotti, Andrea Salvatori, Guglielmo Rigon, Giuliano Signore, Fabrizio Pietrantoni, Emanuela Scapaticci, Margherita Lante, Isabella Goffredo, Bianca Maria Mazzina, Oscar Elbousify, Ahmed Ibrahim Roncada, Paola Dotta, Andrea Fiocchi, Alessandro Putignani, Lorenza |
author_facet | Di Francesco, Laura Di Girolamo, Francesco Mennini, Maurizio Masotti, Andrea Salvatori, Guglielmo Rigon, Giuliano Signore, Fabrizio Pietrantoni, Emanuela Scapaticci, Margherita Lante, Isabella Goffredo, Bianca Maria Mazzina, Oscar Elbousify, Ahmed Ibrahim Roncada, Paola Dotta, Andrea Fiocchi, Alessandro Putignani, Lorenza |
author_sort | Di Francesco, Laura |
collection | PubMed |
description | Human milk composition is dynamic, and substitute formulae are intended to mimic its protein content. The purpose of this study was to investigate the potentiality of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), followed by multivariate data analyses as a tool to analyze the peptide profiles of mammalian, human, and formula milks. Breast milk samples from women at different lactation stages (2 (n = 5), 30 (n = 6), 60 (n = 5), and 90 (n = 4) days postpartum), and milk from donkeys (n = 4), cows (n = 4), buffaloes (n = 7), goats (n = 4), ewes (n = 5), and camels (n = 2) were collected. Different brands (n = 4) of infant formulae were also analyzed. Protein content (<30 kDa) was analyzed by MS, and data were exported for statistical elaborations. The mass spectra for each milk closely clustered together, whereas different milk samples resulted in well-separated mass spectra. Human samples formed a cluster in which colostrum constituted a well-defined subcluster. None of the milk formulae correlated with animal or human milk, although they were specifically characterized and correlated well with each other. These findings propose MALDI-TOF MS milk profiling as an analytical tool to discriminate, in a blinded way, different milk types. As each formula has a distinct specificity, shifting a baby from one to another formula implies a specific proteomic exposure. These profiles may assist in milk proteomics for easiness of use and minimization of costs, suggesting that the MALDI-TOF MS pipelines may be useful for not only milk adulteration assessments but also for the characterization of banked milk specimens in pediatric clinical settings. |
format | Online Article Text |
id | pubmed-6163840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61638402018-10-10 A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling Di Francesco, Laura Di Girolamo, Francesco Mennini, Maurizio Masotti, Andrea Salvatori, Guglielmo Rigon, Giuliano Signore, Fabrizio Pietrantoni, Emanuela Scapaticci, Margherita Lante, Isabella Goffredo, Bianca Maria Mazzina, Oscar Elbousify, Ahmed Ibrahim Roncada, Paola Dotta, Andrea Fiocchi, Alessandro Putignani, Lorenza Nutrients Article Human milk composition is dynamic, and substitute formulae are intended to mimic its protein content. The purpose of this study was to investigate the potentiality of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), followed by multivariate data analyses as a tool to analyze the peptide profiles of mammalian, human, and formula milks. Breast milk samples from women at different lactation stages (2 (n = 5), 30 (n = 6), 60 (n = 5), and 90 (n = 4) days postpartum), and milk from donkeys (n = 4), cows (n = 4), buffaloes (n = 7), goats (n = 4), ewes (n = 5), and camels (n = 2) were collected. Different brands (n = 4) of infant formulae were also analyzed. Protein content (<30 kDa) was analyzed by MS, and data were exported for statistical elaborations. The mass spectra for each milk closely clustered together, whereas different milk samples resulted in well-separated mass spectra. Human samples formed a cluster in which colostrum constituted a well-defined subcluster. None of the milk formulae correlated with animal or human milk, although they were specifically characterized and correlated well with each other. These findings propose MALDI-TOF MS milk profiling as an analytical tool to discriminate, in a blinded way, different milk types. As each formula has a distinct specificity, shifting a baby from one to another formula implies a specific proteomic exposure. These profiles may assist in milk proteomics for easiness of use and minimization of costs, suggesting that the MALDI-TOF MS pipelines may be useful for not only milk adulteration assessments but also for the characterization of banked milk specimens in pediatric clinical settings. MDPI 2018-09-05 /pmc/articles/PMC6163840/ /pubmed/30189627 http://dx.doi.org/10.3390/nu10091238 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Di Francesco, Laura Di Girolamo, Francesco Mennini, Maurizio Masotti, Andrea Salvatori, Guglielmo Rigon, Giuliano Signore, Fabrizio Pietrantoni, Emanuela Scapaticci, Margherita Lante, Isabella Goffredo, Bianca Maria Mazzina, Oscar Elbousify, Ahmed Ibrahim Roncada, Paola Dotta, Andrea Fiocchi, Alessandro Putignani, Lorenza A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling |
title | A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling |
title_full | A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling |
title_fullStr | A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling |
title_full_unstemmed | A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling |
title_short | A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling |
title_sort | maldi-tof ms approach for mammalian, human, and formula milks’ profiling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163840/ https://www.ncbi.nlm.nih.gov/pubmed/30189627 http://dx.doi.org/10.3390/nu10091238 |
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