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A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling

Human milk composition is dynamic, and substitute formulae are intended to mimic its protein content. The purpose of this study was to investigate the potentiality of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), followed by multivariate data analyses a...

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Autores principales: Di Francesco, Laura, Di Girolamo, Francesco, Mennini, Maurizio, Masotti, Andrea, Salvatori, Guglielmo, Rigon, Giuliano, Signore, Fabrizio, Pietrantoni, Emanuela, Scapaticci, Margherita, Lante, Isabella, Goffredo, Bianca Maria, Mazzina, Oscar, Elbousify, Ahmed Ibrahim, Roncada, Paola, Dotta, Andrea, Fiocchi, Alessandro, Putignani, Lorenza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163840/
https://www.ncbi.nlm.nih.gov/pubmed/30189627
http://dx.doi.org/10.3390/nu10091238
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author Di Francesco, Laura
Di Girolamo, Francesco
Mennini, Maurizio
Masotti, Andrea
Salvatori, Guglielmo
Rigon, Giuliano
Signore, Fabrizio
Pietrantoni, Emanuela
Scapaticci, Margherita
Lante, Isabella
Goffredo, Bianca Maria
Mazzina, Oscar
Elbousify, Ahmed Ibrahim
Roncada, Paola
Dotta, Andrea
Fiocchi, Alessandro
Putignani, Lorenza
author_facet Di Francesco, Laura
Di Girolamo, Francesco
Mennini, Maurizio
Masotti, Andrea
Salvatori, Guglielmo
Rigon, Giuliano
Signore, Fabrizio
Pietrantoni, Emanuela
Scapaticci, Margherita
Lante, Isabella
Goffredo, Bianca Maria
Mazzina, Oscar
Elbousify, Ahmed Ibrahim
Roncada, Paola
Dotta, Andrea
Fiocchi, Alessandro
Putignani, Lorenza
author_sort Di Francesco, Laura
collection PubMed
description Human milk composition is dynamic, and substitute formulae are intended to mimic its protein content. The purpose of this study was to investigate the potentiality of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), followed by multivariate data analyses as a tool to analyze the peptide profiles of mammalian, human, and formula milks. Breast milk samples from women at different lactation stages (2 (n = 5), 30 (n = 6), 60 (n = 5), and 90 (n = 4) days postpartum), and milk from donkeys (n = 4), cows (n = 4), buffaloes (n = 7), goats (n = 4), ewes (n = 5), and camels (n = 2) were collected. Different brands (n = 4) of infant formulae were also analyzed. Protein content (<30 kDa) was analyzed by MS, and data were exported for statistical elaborations. The mass spectra for each milk closely clustered together, whereas different milk samples resulted in well-separated mass spectra. Human samples formed a cluster in which colostrum constituted a well-defined subcluster. None of the milk formulae correlated with animal or human milk, although they were specifically characterized and correlated well with each other. These findings propose MALDI-TOF MS milk profiling as an analytical tool to discriminate, in a blinded way, different milk types. As each formula has a distinct specificity, shifting a baby from one to another formula implies a specific proteomic exposure. These profiles may assist in milk proteomics for easiness of use and minimization of costs, suggesting that the MALDI-TOF MS pipelines may be useful for not only milk adulteration assessments but also for the characterization of banked milk specimens in pediatric clinical settings.
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spelling pubmed-61638402018-10-10 A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling Di Francesco, Laura Di Girolamo, Francesco Mennini, Maurizio Masotti, Andrea Salvatori, Guglielmo Rigon, Giuliano Signore, Fabrizio Pietrantoni, Emanuela Scapaticci, Margherita Lante, Isabella Goffredo, Bianca Maria Mazzina, Oscar Elbousify, Ahmed Ibrahim Roncada, Paola Dotta, Andrea Fiocchi, Alessandro Putignani, Lorenza Nutrients Article Human milk composition is dynamic, and substitute formulae are intended to mimic its protein content. The purpose of this study was to investigate the potentiality of matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), followed by multivariate data analyses as a tool to analyze the peptide profiles of mammalian, human, and formula milks. Breast milk samples from women at different lactation stages (2 (n = 5), 30 (n = 6), 60 (n = 5), and 90 (n = 4) days postpartum), and milk from donkeys (n = 4), cows (n = 4), buffaloes (n = 7), goats (n = 4), ewes (n = 5), and camels (n = 2) were collected. Different brands (n = 4) of infant formulae were also analyzed. Protein content (<30 kDa) was analyzed by MS, and data were exported for statistical elaborations. The mass spectra for each milk closely clustered together, whereas different milk samples resulted in well-separated mass spectra. Human samples formed a cluster in which colostrum constituted a well-defined subcluster. None of the milk formulae correlated with animal or human milk, although they were specifically characterized and correlated well with each other. These findings propose MALDI-TOF MS milk profiling as an analytical tool to discriminate, in a blinded way, different milk types. As each formula has a distinct specificity, shifting a baby from one to another formula implies a specific proteomic exposure. These profiles may assist in milk proteomics for easiness of use and minimization of costs, suggesting that the MALDI-TOF MS pipelines may be useful for not only milk adulteration assessments but also for the characterization of banked milk specimens in pediatric clinical settings. MDPI 2018-09-05 /pmc/articles/PMC6163840/ /pubmed/30189627 http://dx.doi.org/10.3390/nu10091238 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Francesco, Laura
Di Girolamo, Francesco
Mennini, Maurizio
Masotti, Andrea
Salvatori, Guglielmo
Rigon, Giuliano
Signore, Fabrizio
Pietrantoni, Emanuela
Scapaticci, Margherita
Lante, Isabella
Goffredo, Bianca Maria
Mazzina, Oscar
Elbousify, Ahmed Ibrahim
Roncada, Paola
Dotta, Andrea
Fiocchi, Alessandro
Putignani, Lorenza
A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling
title A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling
title_full A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling
title_fullStr A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling
title_full_unstemmed A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling
title_short A MALDI-TOF MS Approach for Mammalian, Human, and Formula Milks’ Profiling
title_sort maldi-tof ms approach for mammalian, human, and formula milks’ profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163840/
https://www.ncbi.nlm.nih.gov/pubmed/30189627
http://dx.doi.org/10.3390/nu10091238
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