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Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process

Aedes aegypti (Ae. aegypti) is a competent vector for transmitting important viral diseases such as yellow fever, dengue, chikungunya, and Zika. Several strategies have been applied to avoid Ae. aegypti proliferation by using environmental management, biological, and chemical approaches. However, th...

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Autores principales: Lima, Alessandra R., Silva, Cicera M., Caires, Cynthia S. A., Prado, Esmael D., Rocha, Luciana R. P., Cabrini, Isaias, Arruda, Eduardo J., Oliveira, Samuel L., Caires, Anderson R. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163889/
https://www.ncbi.nlm.nih.gov/pubmed/30200177
http://dx.doi.org/10.3390/insects9030109
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author Lima, Alessandra R.
Silva, Cicera M.
Caires, Cynthia S. A.
Prado, Esmael D.
Rocha, Luciana R. P.
Cabrini, Isaias
Arruda, Eduardo J.
Oliveira, Samuel L.
Caires, Anderson R. L.
author_facet Lima, Alessandra R.
Silva, Cicera M.
Caires, Cynthia S. A.
Prado, Esmael D.
Rocha, Luciana R. P.
Cabrini, Isaias
Arruda, Eduardo J.
Oliveira, Samuel L.
Caires, Anderson R. L.
author_sort Lima, Alessandra R.
collection PubMed
description Aedes aegypti (Ae. aegypti) is a competent vector for transmitting important viral diseases such as yellow fever, dengue, chikungunya, and Zika. Several strategies have been applied to avoid Ae. aegypti proliferation by using environmental management, biological, and chemical approaches. However, the development of new methods for effective control of the insect vector population is still needed. Photodynamic control is an alternative way to control the vector population by using a physical approach based on the larval phototoxicity of a photosensitizer. In this context, the present study evaluated the use of eosin-methylene blue (EMB) as a new photosensitizer for photodynamic control of Ae. aegypti larval populations. The photodynamic assays were performed submitting Ae. aegypti third-instar larvae to different EMB concentrations (0.0, 0.5, 1.0, 5.0, 10.0, 50.0, and 100.0 µg mL(−1)) in combination of three different light doses (24.3, 48.6, and 97.2 J cm(−2)) under either white-light radiation from RGB LEDs or sunlight. The results demonstrated that EMB presented a rapid internalization into the larvae and was phototoxic. The photodynamic action induced 100% of larval mortality after about 40 min of sunlight irradiation even using low EMB concentration (0.5 µg mL(−1)). The findings reveal EMB as an effective photoactive compound to control larval populations of Ae. aegypti by photodynamic process induced by either sunlight or white-light from RGB LEDs.
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spelling pubmed-61638892018-10-10 Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process Lima, Alessandra R. Silva, Cicera M. Caires, Cynthia S. A. Prado, Esmael D. Rocha, Luciana R. P. Cabrini, Isaias Arruda, Eduardo J. Oliveira, Samuel L. Caires, Anderson R. L. Insects Article Aedes aegypti (Ae. aegypti) is a competent vector for transmitting important viral diseases such as yellow fever, dengue, chikungunya, and Zika. Several strategies have been applied to avoid Ae. aegypti proliferation by using environmental management, biological, and chemical approaches. However, the development of new methods for effective control of the insect vector population is still needed. Photodynamic control is an alternative way to control the vector population by using a physical approach based on the larval phototoxicity of a photosensitizer. In this context, the present study evaluated the use of eosin-methylene blue (EMB) as a new photosensitizer for photodynamic control of Ae. aegypti larval populations. The photodynamic assays were performed submitting Ae. aegypti third-instar larvae to different EMB concentrations (0.0, 0.5, 1.0, 5.0, 10.0, 50.0, and 100.0 µg mL(−1)) in combination of three different light doses (24.3, 48.6, and 97.2 J cm(−2)) under either white-light radiation from RGB LEDs or sunlight. The results demonstrated that EMB presented a rapid internalization into the larvae and was phototoxic. The photodynamic action induced 100% of larval mortality after about 40 min of sunlight irradiation even using low EMB concentration (0.5 µg mL(−1)). The findings reveal EMB as an effective photoactive compound to control larval populations of Ae. aegypti by photodynamic process induced by either sunlight or white-light from RGB LEDs. MDPI 2018-08-30 /pmc/articles/PMC6163889/ /pubmed/30200177 http://dx.doi.org/10.3390/insects9030109 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lima, Alessandra R.
Silva, Cicera M.
Caires, Cynthia S. A.
Prado, Esmael D.
Rocha, Luciana R. P.
Cabrini, Isaias
Arruda, Eduardo J.
Oliveira, Samuel L.
Caires, Anderson R. L.
Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process
title Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process
title_full Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process
title_fullStr Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process
title_full_unstemmed Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process
title_short Evaluation of Eosin-Methylene Blue as a Photosensitizer for Larval Control of Aedes aegypti by a Photodynamic Process
title_sort evaluation of eosin-methylene blue as a photosensitizer for larval control of aedes aegypti by a photodynamic process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163889/
https://www.ncbi.nlm.nih.gov/pubmed/30200177
http://dx.doi.org/10.3390/insects9030109
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