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Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance

Drought stress significantly limits cotton growth and production due to the necessity of water at every stage of crop growth. Hence, it is essential to identify tolerant genetic resources and understand the mechanisms of drought tolerance in economically and socially important plants such as cotton....

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Autores principales: Hasan, Md Mosfeq-Ul, Ma, Fanglu, Prodhan, Zakaria Hossain, Li, Feng, Shen, Hao, Chen, Yadong, Wang, Xuede
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163957/
https://www.ncbi.nlm.nih.gov/pubmed/30200561
http://dx.doi.org/10.3390/ijms19092636
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author Hasan, Md Mosfeq-Ul
Ma, Fanglu
Prodhan, Zakaria Hossain
Li, Feng
Shen, Hao
Chen, Yadong
Wang, Xuede
author_facet Hasan, Md Mosfeq-Ul
Ma, Fanglu
Prodhan, Zakaria Hossain
Li, Feng
Shen, Hao
Chen, Yadong
Wang, Xuede
author_sort Hasan, Md Mosfeq-Ul
collection PubMed
description Drought stress significantly limits cotton growth and production due to the necessity of water at every stage of crop growth. Hence, it is essential to identify tolerant genetic resources and understand the mechanisms of drought tolerance in economically and socially important plants such as cotton. In this study, molecular and physio-biochemical investigations were conducted by analyzing different parameters by following standard protocols in three different cotton species, namely TM-1 (Gossypium hirsutum), Zhongmian-16 (Gossypium arboreum), and Pima4-S (Gossypium barbadense). Drought stress significantly decreased plant growth, chlorophyll content, net photosynthetic rate (P(n)), stomatal conductance (Gs), maximum photochemical efficiency of PSII (Fv/Fm), and relative water content. TM-1 resulted in more tolerance than the other two species. The accumulation of proline, soluble proteins, soluble sugars, hydrogen peroxide (H(2)O(2)), and superoxide radicals (O(2)(•)(−)) increased significantly in TM-1. In addition, TM-1 maintained the integrity of the chloroplast structure under drought conditions. The relative expression level of drought-responsive genes including coding for transcription factors and other regulatory proteins or enzymes controlling genes (ERF, ERFB, DREB, WRKY6, ZFP1, FeSOD, CuZnSOD, MAPKKK17, P5CR, and PRP5) were higher in TM-1 under drought, conferring a more tolerant status than in Zhongmian-16 and Pima4-S. The findings of this research could be utilized for predicting a tolerant cotton genotype as well as evaluating prospective cotton species in the variety development program.
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spelling pubmed-61639572018-10-10 Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance Hasan, Md Mosfeq-Ul Ma, Fanglu Prodhan, Zakaria Hossain Li, Feng Shen, Hao Chen, Yadong Wang, Xuede Int J Mol Sci Article Drought stress significantly limits cotton growth and production due to the necessity of water at every stage of crop growth. Hence, it is essential to identify tolerant genetic resources and understand the mechanisms of drought tolerance in economically and socially important plants such as cotton. In this study, molecular and physio-biochemical investigations were conducted by analyzing different parameters by following standard protocols in three different cotton species, namely TM-1 (Gossypium hirsutum), Zhongmian-16 (Gossypium arboreum), and Pima4-S (Gossypium barbadense). Drought stress significantly decreased plant growth, chlorophyll content, net photosynthetic rate (P(n)), stomatal conductance (Gs), maximum photochemical efficiency of PSII (Fv/Fm), and relative water content. TM-1 resulted in more tolerance than the other two species. The accumulation of proline, soluble proteins, soluble sugars, hydrogen peroxide (H(2)O(2)), and superoxide radicals (O(2)(•)(−)) increased significantly in TM-1. In addition, TM-1 maintained the integrity of the chloroplast structure under drought conditions. The relative expression level of drought-responsive genes including coding for transcription factors and other regulatory proteins or enzymes controlling genes (ERF, ERFB, DREB, WRKY6, ZFP1, FeSOD, CuZnSOD, MAPKKK17, P5CR, and PRP5) were higher in TM-1 under drought, conferring a more tolerant status than in Zhongmian-16 and Pima4-S. The findings of this research could be utilized for predicting a tolerant cotton genotype as well as evaluating prospective cotton species in the variety development program. MDPI 2018-09-06 /pmc/articles/PMC6163957/ /pubmed/30200561 http://dx.doi.org/10.3390/ijms19092636 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hasan, Md Mosfeq-Ul
Ma, Fanglu
Prodhan, Zakaria Hossain
Li, Feng
Shen, Hao
Chen, Yadong
Wang, Xuede
Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance
title Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance
title_full Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance
title_fullStr Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance
title_full_unstemmed Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance
title_short Molecular and Physio-Biochemical Characterization of Cotton Species for Assessing Drought Stress Tolerance
title_sort molecular and physio-biochemical characterization of cotton species for assessing drought stress tolerance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163957/
https://www.ncbi.nlm.nih.gov/pubmed/30200561
http://dx.doi.org/10.3390/ijms19092636
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