Cargando…

The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis

Atherosclerosis is closely related to vascular dysfunction and hypertension. Ojeoksan (OJS), originally recorded in an ancient Korean medicinal book named “Donguibogam”, is a well-known, blended herbal formula. This study was carried out to investigate the beneficial effects of OJS on atherosclerosi...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Byung Hyuk, Seo, Chang Seob, Yoon, Jung Joo, Kim, Hye Yoom, Ahn, You Mee, Eun, So Young, Hong, Mi Hyeon, Lee, Jae Geon, Shin, Hyeun Kyoo, Lee, Ho Sub, Lee, Yun Jung, Kang, Dae Gill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164175/
https://www.ncbi.nlm.nih.gov/pubmed/30200674
http://dx.doi.org/10.3390/nu10091256
_version_ 1783359536443162624
author Han, Byung Hyuk
Seo, Chang Seob
Yoon, Jung Joo
Kim, Hye Yoom
Ahn, You Mee
Eun, So Young
Hong, Mi Hyeon
Lee, Jae Geon
Shin, Hyeun Kyoo
Lee, Ho Sub
Lee, Yun Jung
Kang, Dae Gill
author_facet Han, Byung Hyuk
Seo, Chang Seob
Yoon, Jung Joo
Kim, Hye Yoom
Ahn, You Mee
Eun, So Young
Hong, Mi Hyeon
Lee, Jae Geon
Shin, Hyeun Kyoo
Lee, Ho Sub
Lee, Yun Jung
Kang, Dae Gill
author_sort Han, Byung Hyuk
collection PubMed
description Atherosclerosis is closely related to vascular dysfunction and hypertension. Ojeoksan (OJS), originally recorded in an ancient Korean medicinal book named “Donguibogam”, is a well-known, blended herbal formula. This study was carried out to investigate the beneficial effects of OJS on atherosclerosis in vitro and in vivo. Western-diet-fed apolipoprotein-E gene-deficient mice (ApoE −/−) were used for this study for 16 weeks, and their vascular dysfunction and inflammation were analyzed. OJS-treated ApoE −/− mice showed lowered blood pressure and glucose levels. The levels of metabolic parameters with hyperlipidemia attenuated following OJS administration. Hematoxylin and eosin (H&E) staining revealed that treatment with OJS reduced atherosclerotic lesions. OJS also suppressed the expression of adhesion molecules and matrix metalloproteinases (MMPs) compared to Western-diet-fed ApoE −/− mice and tumor necrosis factor-alpha (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs). Expression levels of MicroRNAs (miRNA)-10a, -126 3p were increased in OJS-fed ApoE −/− mice. OJS significantly increased the phosphorylation of endothelial nitric oxide synthase (eNOS) and protein kinase B (Akt), which are involved in nitric oxide (NO) production. OJS also regulated eNOS coupling by increasing the expression of endothelial GTP Cyclohydrolase-1 (GTPCH). Taken together, OJS has a protective effect on vascular inflammation via eNOS coupling-mediated NO production and might be a potential therapeutic agent for both early and advanced atherosclerosis.
format Online
Article
Text
id pubmed-6164175
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61641752018-10-10 The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis Han, Byung Hyuk Seo, Chang Seob Yoon, Jung Joo Kim, Hye Yoom Ahn, You Mee Eun, So Young Hong, Mi Hyeon Lee, Jae Geon Shin, Hyeun Kyoo Lee, Ho Sub Lee, Yun Jung Kang, Dae Gill Nutrients Article Atherosclerosis is closely related to vascular dysfunction and hypertension. Ojeoksan (OJS), originally recorded in an ancient Korean medicinal book named “Donguibogam”, is a well-known, blended herbal formula. This study was carried out to investigate the beneficial effects of OJS on atherosclerosis in vitro and in vivo. Western-diet-fed apolipoprotein-E gene-deficient mice (ApoE −/−) were used for this study for 16 weeks, and their vascular dysfunction and inflammation were analyzed. OJS-treated ApoE −/− mice showed lowered blood pressure and glucose levels. The levels of metabolic parameters with hyperlipidemia attenuated following OJS administration. Hematoxylin and eosin (H&E) staining revealed that treatment with OJS reduced atherosclerotic lesions. OJS also suppressed the expression of adhesion molecules and matrix metalloproteinases (MMPs) compared to Western-diet-fed ApoE −/− mice and tumor necrosis factor-alpha (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs). Expression levels of MicroRNAs (miRNA)-10a, -126 3p were increased in OJS-fed ApoE −/− mice. OJS significantly increased the phosphorylation of endothelial nitric oxide synthase (eNOS) and protein kinase B (Akt), which are involved in nitric oxide (NO) production. OJS also regulated eNOS coupling by increasing the expression of endothelial GTP Cyclohydrolase-1 (GTPCH). Taken together, OJS has a protective effect on vascular inflammation via eNOS coupling-mediated NO production and might be a potential therapeutic agent for both early and advanced atherosclerosis. MDPI 2018-09-06 /pmc/articles/PMC6164175/ /pubmed/30200674 http://dx.doi.org/10.3390/nu10091256 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Byung Hyuk
Seo, Chang Seob
Yoon, Jung Joo
Kim, Hye Yoom
Ahn, You Mee
Eun, So Young
Hong, Mi Hyeon
Lee, Jae Geon
Shin, Hyeun Kyoo
Lee, Ho Sub
Lee, Yun Jung
Kang, Dae Gill
The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
title The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
title_full The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
title_fullStr The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
title_full_unstemmed The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
title_short The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
title_sort inhibitory effect of ojeoksan on early and advanced atherosclerosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164175/
https://www.ncbi.nlm.nih.gov/pubmed/30200674
http://dx.doi.org/10.3390/nu10091256
work_keys_str_mv AT hanbyunghyuk theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT seochangseob theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT yoonjungjoo theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT kimhyeyoom theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT ahnyoumee theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT eunsoyoung theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT hongmihyeon theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT leejaegeon theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT shinhyeunkyoo theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT leehosub theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT leeyunjung theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT kangdaegill theinhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT hanbyunghyuk inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT seochangseob inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT yoonjungjoo inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT kimhyeyoom inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT ahnyoumee inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT eunsoyoung inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT hongmihyeon inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT leejaegeon inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT shinhyeunkyoo inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT leehosub inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT leeyunjung inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis
AT kangdaegill inhibitoryeffectofojeoksanonearlyandadvancedatherosclerosis