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The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis
Atherosclerosis is closely related to vascular dysfunction and hypertension. Ojeoksan (OJS), originally recorded in an ancient Korean medicinal book named “Donguibogam”, is a well-known, blended herbal formula. This study was carried out to investigate the beneficial effects of OJS on atherosclerosi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164175/ https://www.ncbi.nlm.nih.gov/pubmed/30200674 http://dx.doi.org/10.3390/nu10091256 |
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author | Han, Byung Hyuk Seo, Chang Seob Yoon, Jung Joo Kim, Hye Yoom Ahn, You Mee Eun, So Young Hong, Mi Hyeon Lee, Jae Geon Shin, Hyeun Kyoo Lee, Ho Sub Lee, Yun Jung Kang, Dae Gill |
author_facet | Han, Byung Hyuk Seo, Chang Seob Yoon, Jung Joo Kim, Hye Yoom Ahn, You Mee Eun, So Young Hong, Mi Hyeon Lee, Jae Geon Shin, Hyeun Kyoo Lee, Ho Sub Lee, Yun Jung Kang, Dae Gill |
author_sort | Han, Byung Hyuk |
collection | PubMed |
description | Atherosclerosis is closely related to vascular dysfunction and hypertension. Ojeoksan (OJS), originally recorded in an ancient Korean medicinal book named “Donguibogam”, is a well-known, blended herbal formula. This study was carried out to investigate the beneficial effects of OJS on atherosclerosis in vitro and in vivo. Western-diet-fed apolipoprotein-E gene-deficient mice (ApoE −/−) were used for this study for 16 weeks, and their vascular dysfunction and inflammation were analyzed. OJS-treated ApoE −/− mice showed lowered blood pressure and glucose levels. The levels of metabolic parameters with hyperlipidemia attenuated following OJS administration. Hematoxylin and eosin (H&E) staining revealed that treatment with OJS reduced atherosclerotic lesions. OJS also suppressed the expression of adhesion molecules and matrix metalloproteinases (MMPs) compared to Western-diet-fed ApoE −/− mice and tumor necrosis factor-alpha (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs). Expression levels of MicroRNAs (miRNA)-10a, -126 3p were increased in OJS-fed ApoE −/− mice. OJS significantly increased the phosphorylation of endothelial nitric oxide synthase (eNOS) and protein kinase B (Akt), which are involved in nitric oxide (NO) production. OJS also regulated eNOS coupling by increasing the expression of endothelial GTP Cyclohydrolase-1 (GTPCH). Taken together, OJS has a protective effect on vascular inflammation via eNOS coupling-mediated NO production and might be a potential therapeutic agent for both early and advanced atherosclerosis. |
format | Online Article Text |
id | pubmed-6164175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61641752018-10-10 The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis Han, Byung Hyuk Seo, Chang Seob Yoon, Jung Joo Kim, Hye Yoom Ahn, You Mee Eun, So Young Hong, Mi Hyeon Lee, Jae Geon Shin, Hyeun Kyoo Lee, Ho Sub Lee, Yun Jung Kang, Dae Gill Nutrients Article Atherosclerosis is closely related to vascular dysfunction and hypertension. Ojeoksan (OJS), originally recorded in an ancient Korean medicinal book named “Donguibogam”, is a well-known, blended herbal formula. This study was carried out to investigate the beneficial effects of OJS on atherosclerosis in vitro and in vivo. Western-diet-fed apolipoprotein-E gene-deficient mice (ApoE −/−) were used for this study for 16 weeks, and their vascular dysfunction and inflammation were analyzed. OJS-treated ApoE −/− mice showed lowered blood pressure and glucose levels. The levels of metabolic parameters with hyperlipidemia attenuated following OJS administration. Hematoxylin and eosin (H&E) staining revealed that treatment with OJS reduced atherosclerotic lesions. OJS also suppressed the expression of adhesion molecules and matrix metalloproteinases (MMPs) compared to Western-diet-fed ApoE −/− mice and tumor necrosis factor-alpha (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs). Expression levels of MicroRNAs (miRNA)-10a, -126 3p were increased in OJS-fed ApoE −/− mice. OJS significantly increased the phosphorylation of endothelial nitric oxide synthase (eNOS) and protein kinase B (Akt), which are involved in nitric oxide (NO) production. OJS also regulated eNOS coupling by increasing the expression of endothelial GTP Cyclohydrolase-1 (GTPCH). Taken together, OJS has a protective effect on vascular inflammation via eNOS coupling-mediated NO production and might be a potential therapeutic agent for both early and advanced atherosclerosis. MDPI 2018-09-06 /pmc/articles/PMC6164175/ /pubmed/30200674 http://dx.doi.org/10.3390/nu10091256 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Byung Hyuk Seo, Chang Seob Yoon, Jung Joo Kim, Hye Yoom Ahn, You Mee Eun, So Young Hong, Mi Hyeon Lee, Jae Geon Shin, Hyeun Kyoo Lee, Ho Sub Lee, Yun Jung Kang, Dae Gill The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis |
title | The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis |
title_full | The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis |
title_fullStr | The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis |
title_full_unstemmed | The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis |
title_short | The Inhibitory Effect of Ojeoksan on Early and Advanced Atherosclerosis |
title_sort | inhibitory effect of ojeoksan on early and advanced atherosclerosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164175/ https://www.ncbi.nlm.nih.gov/pubmed/30200674 http://dx.doi.org/10.3390/nu10091256 |
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