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Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae
Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164196/ https://www.ncbi.nlm.nih.gov/pubmed/30081551 http://dx.doi.org/10.3390/antibiotics7030070 |
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| author | Perera, Sumudu R Taheri, Ali Khan, Nurul H Parti, Rajinder P Stefura, Stephanie Skiba, Pauline Acker, Jason P Martin, Irene Kusalik, Anthony Dillon, Jo-Anne R |
| author_facet | Perera, Sumudu R Taheri, Ali Khan, Nurul H Parti, Rajinder P Stefura, Stephanie Skiba, Pauline Acker, Jason P Martin, Irene Kusalik, Anthony Dillon, Jo-Anne R |
| author_sort | Perera, Sumudu R |
| collection | PubMed |
| description | Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis. |
| format | Online Article Text |
| id | pubmed-6164196 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| publisher | MDPI |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-61641962018-10-12 Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae Perera, Sumudu R Taheri, Ali Khan, Nurul H Parti, Rajinder P Stefura, Stephanie Skiba, Pauline Acker, Jason P Martin, Irene Kusalik, Anthony Dillon, Jo-Anne R Antibiotics (Basel) Article Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis. MDPI 2018-08-04 /pmc/articles/PMC6164196/ /pubmed/30081551 http://dx.doi.org/10.3390/antibiotics7030070 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article Perera, Sumudu R Taheri, Ali Khan, Nurul H Parti, Rajinder P Stefura, Stephanie Skiba, Pauline Acker, Jason P Martin, Irene Kusalik, Anthony Dillon, Jo-Anne R Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae |
| title | Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae |
| title_full | Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae |
| title_fullStr | Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae |
| title_full_unstemmed | Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae |
| title_short | Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae |
| title_sort | evaluation of a hydrogel-based diagnostic approach for the point-of-care based detection of neisseria gonorrhoeae |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164196/ https://www.ncbi.nlm.nih.gov/pubmed/30081551 http://dx.doi.org/10.3390/antibiotics7030070 |
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