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Protective Actions of Anserine Under Diabetic Conditions

Background/Aims: In rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, the methylated form of carnosine. Methods: Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human rena...

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Autores principales: Peters, Verena, Calabrese, Vittorio, Forsberg, Elisabete, Volk, Nadine, Fleming, Thomas, Baelde, Hans, Weigand, Tim, Thiel, Christian, Trovato, Angela, Scuto, Maria, Modafferi, Sergio, Schmitt, Claus Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164239/
https://www.ncbi.nlm.nih.gov/pubmed/30217069
http://dx.doi.org/10.3390/ijms19092751
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author Peters, Verena
Calabrese, Vittorio
Forsberg, Elisabete
Volk, Nadine
Fleming, Thomas
Baelde, Hans
Weigand, Tim
Thiel, Christian
Trovato, Angela
Scuto, Maria
Modafferi, Sergio
Schmitt, Claus Peter
author_facet Peters, Verena
Calabrese, Vittorio
Forsberg, Elisabete
Volk, Nadine
Fleming, Thomas
Baelde, Hans
Weigand, Tim
Thiel, Christian
Trovato, Angela
Scuto, Maria
Modafferi, Sergio
Schmitt, Claus Peter
author_sort Peters, Verena
collection PubMed
description Background/Aims: In rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, the methylated form of carnosine. Methods: Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting. In wildtype (WT) and diabetic mice (db/db), the effect of short-term anserine treatment on blood glucose, proteinuria and vascular permeability was measured. Results: Anserine has a higher antioxidant capacity compared to carnosine (p < 0.001). In tubular cells (HK-2) stressed with 25 mM glucose or 20–100 µM hydrogen peroxide, anserine but not carnosine, increased intracellular heat shock protein (Hsp70) mRNA and protein levels. In HK-2 cells stressed with glucose, co-incubation with anserine also increased hemeoxygenase (HO-1) protein and reduced total protein carbonylation, but had no effect on cellular sirtuin-1 and thioredoxin protein concentrations. Three intravenous anserine injections every 48 h in 12-week-old db/db mice, improved blood glucose by one fifth, vascular permeability by one third, and halved proteinuria (all p < 0.05). Conclusion: Anserine is a potent antioxidant and activates the intracellular Hsp70/HO-1 defense system under oxidative and glycative stress. Short-term anserine treatment in diabetic mice improves glucose homeostasis and nephropathy.
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spelling pubmed-61642392018-10-10 Protective Actions of Anserine Under Diabetic Conditions Peters, Verena Calabrese, Vittorio Forsberg, Elisabete Volk, Nadine Fleming, Thomas Baelde, Hans Weigand, Tim Thiel, Christian Trovato, Angela Scuto, Maria Modafferi, Sergio Schmitt, Claus Peter Int J Mol Sci Article Background/Aims: In rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, the methylated form of carnosine. Methods: Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting. In wildtype (WT) and diabetic mice (db/db), the effect of short-term anserine treatment on blood glucose, proteinuria and vascular permeability was measured. Results: Anserine has a higher antioxidant capacity compared to carnosine (p < 0.001). In tubular cells (HK-2) stressed with 25 mM glucose or 20–100 µM hydrogen peroxide, anserine but not carnosine, increased intracellular heat shock protein (Hsp70) mRNA and protein levels. In HK-2 cells stressed with glucose, co-incubation with anserine also increased hemeoxygenase (HO-1) protein and reduced total protein carbonylation, but had no effect on cellular sirtuin-1 and thioredoxin protein concentrations. Three intravenous anserine injections every 48 h in 12-week-old db/db mice, improved blood glucose by one fifth, vascular permeability by one third, and halved proteinuria (all p < 0.05). Conclusion: Anserine is a potent antioxidant and activates the intracellular Hsp70/HO-1 defense system under oxidative and glycative stress. Short-term anserine treatment in diabetic mice improves glucose homeostasis and nephropathy. MDPI 2018-09-13 /pmc/articles/PMC6164239/ /pubmed/30217069 http://dx.doi.org/10.3390/ijms19092751 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peters, Verena
Calabrese, Vittorio
Forsberg, Elisabete
Volk, Nadine
Fleming, Thomas
Baelde, Hans
Weigand, Tim
Thiel, Christian
Trovato, Angela
Scuto, Maria
Modafferi, Sergio
Schmitt, Claus Peter
Protective Actions of Anserine Under Diabetic Conditions
title Protective Actions of Anserine Under Diabetic Conditions
title_full Protective Actions of Anserine Under Diabetic Conditions
title_fullStr Protective Actions of Anserine Under Diabetic Conditions
title_full_unstemmed Protective Actions of Anserine Under Diabetic Conditions
title_short Protective Actions of Anserine Under Diabetic Conditions
title_sort protective actions of anserine under diabetic conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164239/
https://www.ncbi.nlm.nih.gov/pubmed/30217069
http://dx.doi.org/10.3390/ijms19092751
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