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Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers

Silymarin-loaded mucoadhesive microbeads of Chitosan-MMT were developed using the ionotropic gelation technique. Characterization of the microbeads was performed by DSC, XRD, SEM, and FTIR techniques. In vitro mucoadhesion and drug release studies; gastroprotective studies including the measurement...

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Autores principales: Sharma, Ameya, Puri, Vivek, Kakkar, Vandita, Singh, Inderbir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164251/
https://www.ncbi.nlm.nih.gov/pubmed/30201932
http://dx.doi.org/10.3390/jfb9030052
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author Sharma, Ameya
Puri, Vivek
Kakkar, Vandita
Singh, Inderbir
author_facet Sharma, Ameya
Puri, Vivek
Kakkar, Vandita
Singh, Inderbir
author_sort Sharma, Ameya
collection PubMed
description Silymarin-loaded mucoadhesive microbeads of Chitosan-MMT were developed using the ionotropic gelation technique. Characterization of the microbeads was performed by DSC, XRD, SEM, and FTIR techniques. In vitro mucoadhesion and drug release studies; gastroprotective studies including the measurement of ulcerative index; the determination of gastric wall mucus; and the determination of percentage protection, biochemical, and histopathological studies were also performed. Microbeads batches were evaluated for particle size (120–140 µm), actual drug content, (49.36–58.18%) and entrapment efficiency (72.52–92.39%).Biochemical estimation of myeloperoxidase was found to be 0.10–0.75 µmoles/g/tissue. Significant reduction in the ulcerative index showed the gastroprotective effect of the formulation. Silymarin-loaded beads of Chitosan-MMT were found to exhibit good mucoadhesion and efficient release of the drug, and were found to be a promising drug carrier system for the treatment of gastric ulcers.
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spelling pubmed-61642512018-10-12 Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers Sharma, Ameya Puri, Vivek Kakkar, Vandita Singh, Inderbir J Funct Biomater Article Silymarin-loaded mucoadhesive microbeads of Chitosan-MMT were developed using the ionotropic gelation technique. Characterization of the microbeads was performed by DSC, XRD, SEM, and FTIR techniques. In vitro mucoadhesion and drug release studies; gastroprotective studies including the measurement of ulcerative index; the determination of gastric wall mucus; and the determination of percentage protection, biochemical, and histopathological studies were also performed. Microbeads batches were evaluated for particle size (120–140 µm), actual drug content, (49.36–58.18%) and entrapment efficiency (72.52–92.39%).Biochemical estimation of myeloperoxidase was found to be 0.10–0.75 µmoles/g/tissue. Significant reduction in the ulcerative index showed the gastroprotective effect of the formulation. Silymarin-loaded beads of Chitosan-MMT were found to exhibit good mucoadhesion and efficient release of the drug, and were found to be a promising drug carrier system for the treatment of gastric ulcers. MDPI 2018-09-10 /pmc/articles/PMC6164251/ /pubmed/30201932 http://dx.doi.org/10.3390/jfb9030052 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sharma, Ameya
Puri, Vivek
Kakkar, Vandita
Singh, Inderbir
Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers
title Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers
title_full Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers
title_fullStr Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers
title_full_unstemmed Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers
title_short Formulation and Evaluation of Silymarin-Loaded Chitosan-Montmorilloite Microbeads for the Potential Treatment of Gastric Ulcers
title_sort formulation and evaluation of silymarin-loaded chitosan-montmorilloite microbeads for the potential treatment of gastric ulcers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164251/
https://www.ncbi.nlm.nih.gov/pubmed/30201932
http://dx.doi.org/10.3390/jfb9030052
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