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Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensi...

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Autores principales: Farias, Chantell, Lyman, Roman, Hemingway, Cecilia, Chau, Huong, Mahacek, Anne, Bouzos, Evangelia, Mobed-Miremadi, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164407/
https://www.ncbi.nlm.nih.gov/pubmed/30065227
http://dx.doi.org/10.3390/bioengineering5030059
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author Farias, Chantell
Lyman, Roman
Hemingway, Cecilia
Chau, Huong
Mahacek, Anne
Bouzos, Evangelia
Mobed-Miremadi, Maryam
author_facet Farias, Chantell
Lyman, Roman
Hemingway, Cecilia
Chau, Huong
Mahacek, Anne
Bouzos, Evangelia
Mobed-Miremadi, Maryam
author_sort Farias, Chantell
collection PubMed
description Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensional (3D) printed methacrylate-based custom hollow microneedle assembly (circular array of 13 conical frusta) fabricated using stereolithography. With a jetting reliability of 80%, the solvent-sterilized device with a root mean square roughness of 158 nm at the extrusion nozzle tip (d = 325 μm) was operated at a flowrate of 12 mL/min. There was no significant difference between the viability of the sheared and control samples for extrusion times of 2 h (p = 0.14, α = 0.05) and 24 h (p = 0.5, α = 0.05) post-atomization. Factoring the increase in extrusion yield from 21.2% to 56.4% attributed to hydrogel bioerosion quantifiable by a loss in resilience from 5470 (J/m(3)) to 3250 (J/m(3)), there was no significant difference in percentage relative payload (p = 0.2628, α = 0.05) when extrusion occurred 24 h (12.2 ± 4.9%) when compared to 2 h (9.9 ± 2.8%) post-atomization. Results from this paper highlight the feasibility of encapsulated cell extrusion, specifically protection from shear, through a hollow microneedle assembly reported for the first time in literature.
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spelling pubmed-61644072018-10-11 Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion Farias, Chantell Lyman, Roman Hemingway, Cecilia Chau, Huong Mahacek, Anne Bouzos, Evangelia Mobed-Miremadi, Maryam Bioengineering (Basel) Article Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensional (3D) printed methacrylate-based custom hollow microneedle assembly (circular array of 13 conical frusta) fabricated using stereolithography. With a jetting reliability of 80%, the solvent-sterilized device with a root mean square roughness of 158 nm at the extrusion nozzle tip (d = 325 μm) was operated at a flowrate of 12 mL/min. There was no significant difference between the viability of the sheared and control samples for extrusion times of 2 h (p = 0.14, α = 0.05) and 24 h (p = 0.5, α = 0.05) post-atomization. Factoring the increase in extrusion yield from 21.2% to 56.4% attributed to hydrogel bioerosion quantifiable by a loss in resilience from 5470 (J/m(3)) to 3250 (J/m(3)), there was no significant difference in percentage relative payload (p = 0.2628, α = 0.05) when extrusion occurred 24 h (12.2 ± 4.9%) when compared to 2 h (9.9 ± 2.8%) post-atomization. Results from this paper highlight the feasibility of encapsulated cell extrusion, specifically protection from shear, through a hollow microneedle assembly reported for the first time in literature. MDPI 2018-07-31 /pmc/articles/PMC6164407/ /pubmed/30065227 http://dx.doi.org/10.3390/bioengineering5030059 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Farias, Chantell
Lyman, Roman
Hemingway, Cecilia
Chau, Huong
Mahacek, Anne
Bouzos, Evangelia
Mobed-Miremadi, Maryam
Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion
title Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion
title_full Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion
title_fullStr Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion
title_full_unstemmed Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion
title_short Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion
title_sort three-dimensional (3d) printed microneedles for microencapsulated cell extrusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164407/
https://www.ncbi.nlm.nih.gov/pubmed/30065227
http://dx.doi.org/10.3390/bioengineering5030059
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