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Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes

This paper reports the work of developing one coplanar microfluidic sorter while using the electro-wetting on dielectrics (EWOD) technique. When connected with delivery capillary to receive sample solution containing micro-particles, this device can select about 10 micro-particles in high volume thr...

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Detalles Bibliográficos
Autores principales: Chen, Yuanyu, Wang, Shauchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164432/
https://www.ncbi.nlm.nih.gov/pubmed/30181484
http://dx.doi.org/10.3390/s18092941
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author Chen, Yuanyu
Wang, Shauchun
author_facet Chen, Yuanyu
Wang, Shauchun
author_sort Chen, Yuanyu
collection PubMed
description This paper reports the work of developing one coplanar microfluidic sorter while using the electro-wetting on dielectrics (EWOD) technique. When connected with delivery capillary to receive sample solution containing micro-particles, this device can select about 10 micro-particles in high volume throughput of milliliter amount within 20 min, to potentially match the requirement of efficiently determining the low amounts of bacteria in concentrated food and environmental samples, of which the typical bacteria density is 10 colony forming unit or less, much smaller than that of clinical pathogen samples. This coplanar T-shape EWOD device contains two fluidic channels, one inlet channel and the other collection channel stemmed from the middle of inlet channel. When the solution droplet falls from the delivery capillary to the entrance end of inlet channel, the droplet is driven to the intersection of two channels. The droplet containing fluorescent particle will be diverted to the lower channel to collect. Otherwise, the non-fluorescent droplet keeps moving toward the other end of inlet channel to waste zone. The particle fluorescence is collected through microscope lens to detect with one photomultiplier tube. The detected signals trigger the personal computer control board to active each EWOD electrode to direct the droplet moving directions. When the solution of 1 mL containing about 10 fluorescent micro-particles is delivered into this sorting device, nearly all the particles were correctly directed into collection zone in 20 min.
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spelling pubmed-61644322018-10-10 Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes Chen, Yuanyu Wang, Shauchun Sensors (Basel) Article This paper reports the work of developing one coplanar microfluidic sorter while using the electro-wetting on dielectrics (EWOD) technique. When connected with delivery capillary to receive sample solution containing micro-particles, this device can select about 10 micro-particles in high volume throughput of milliliter amount within 20 min, to potentially match the requirement of efficiently determining the low amounts of bacteria in concentrated food and environmental samples, of which the typical bacteria density is 10 colony forming unit or less, much smaller than that of clinical pathogen samples. This coplanar T-shape EWOD device contains two fluidic channels, one inlet channel and the other collection channel stemmed from the middle of inlet channel. When the solution droplet falls from the delivery capillary to the entrance end of inlet channel, the droplet is driven to the intersection of two channels. The droplet containing fluorescent particle will be diverted to the lower channel to collect. Otherwise, the non-fluorescent droplet keeps moving toward the other end of inlet channel to waste zone. The particle fluorescence is collected through microscope lens to detect with one photomultiplier tube. The detected signals trigger the personal computer control board to active each EWOD electrode to direct the droplet moving directions. When the solution of 1 mL containing about 10 fluorescent micro-particles is delivered into this sorting device, nearly all the particles were correctly directed into collection zone in 20 min. MDPI 2018-09-04 /pmc/articles/PMC6164432/ /pubmed/30181484 http://dx.doi.org/10.3390/s18092941 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yuanyu
Wang, Shauchun
Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes
title Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes
title_full Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes
title_fullStr Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes
title_full_unstemmed Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes
title_short Development of Coplanar Electro-Wetting Based Microfluidic Sorter to Select Micro-Particles in High Volume Throughput at Milliliter Amount within Twenty Minutes
title_sort development of coplanar electro-wetting based microfluidic sorter to select micro-particles in high volume throughput at milliliter amount within twenty minutes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164432/
https://www.ncbi.nlm.nih.gov/pubmed/30181484
http://dx.doi.org/10.3390/s18092941
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