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Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds

Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In t...

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Autores principales: Yin, Jun, Seo, Chang-Seob, Hwang, In Hyeok, Lee, Min Won, Song, Kwang Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164478/
https://www.ncbi.nlm.nih.gov/pubmed/30177649
http://dx.doi.org/10.3390/nu10091221
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author Yin, Jun
Seo, Chang-Seob
Hwang, In Hyeok
Lee, Min Won
Song, Kwang Hoon
author_facet Yin, Jun
Seo, Chang-Seob
Hwang, In Hyeok
Lee, Min Won
Song, Kwang Hoon
author_sort Yin, Jun
collection PubMed
description Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In the present work, we studyed the molecular mechanism for the inhibitory effect of DLL-Ex or Chikusetsusaponin IVa (CS-IVa), as isolated from Dolichos lablab L. (DLL) seeds extract, on adipocyte differentiation. We evaluated the effect of DLL-Ex, an anti-obesity agent, and CS-IVa, an active component of DLL-Ex, on 3T3-L1 cell differentiation via Oil red O assay and Q-PCR, along with their effects on CCAAT element binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FAS), and fatty acid-binding protein 4 (FABP4) mRNA transcriptions. FAS and FABP4 protein expression levels after exposure to CS-IVa were also tested. The results showed that DLL-Ex and CS-IVa have potent inhibitory activity on adipocyte differentiation. Therefore, DLL and CS-IVa may be developed as a functional food material to treat obesity.
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spelling pubmed-61644782018-10-10 Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds Yin, Jun Seo, Chang-Seob Hwang, In Hyeok Lee, Min Won Song, Kwang Hoon Nutrients Article Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In the present work, we studyed the molecular mechanism for the inhibitory effect of DLL-Ex or Chikusetsusaponin IVa (CS-IVa), as isolated from Dolichos lablab L. (DLL) seeds extract, on adipocyte differentiation. We evaluated the effect of DLL-Ex, an anti-obesity agent, and CS-IVa, an active component of DLL-Ex, on 3T3-L1 cell differentiation via Oil red O assay and Q-PCR, along with their effects on CCAAT element binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FAS), and fatty acid-binding protein 4 (FABP4) mRNA transcriptions. FAS and FABP4 protein expression levels after exposure to CS-IVa were also tested. The results showed that DLL-Ex and CS-IVa have potent inhibitory activity on adipocyte differentiation. Therefore, DLL and CS-IVa may be developed as a functional food material to treat obesity. MDPI 2018-09-03 /pmc/articles/PMC6164478/ /pubmed/30177649 http://dx.doi.org/10.3390/nu10091221 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yin, Jun
Seo, Chang-Seob
Hwang, In Hyeok
Lee, Min Won
Song, Kwang Hoon
Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
title Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
title_full Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
title_fullStr Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
title_full_unstemmed Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
title_short Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
title_sort anti-obesity activities of chikusetsusaponin iva and dolichos lablab l. seeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164478/
https://www.ncbi.nlm.nih.gov/pubmed/30177649
http://dx.doi.org/10.3390/nu10091221
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