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Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164478/ https://www.ncbi.nlm.nih.gov/pubmed/30177649 http://dx.doi.org/10.3390/nu10091221 |
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author | Yin, Jun Seo, Chang-Seob Hwang, In Hyeok Lee, Min Won Song, Kwang Hoon |
author_facet | Yin, Jun Seo, Chang-Seob Hwang, In Hyeok Lee, Min Won Song, Kwang Hoon |
author_sort | Yin, Jun |
collection | PubMed |
description | Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In the present work, we studyed the molecular mechanism for the inhibitory effect of DLL-Ex or Chikusetsusaponin IVa (CS-IVa), as isolated from Dolichos lablab L. (DLL) seeds extract, on adipocyte differentiation. We evaluated the effect of DLL-Ex, an anti-obesity agent, and CS-IVa, an active component of DLL-Ex, on 3T3-L1 cell differentiation via Oil red O assay and Q-PCR, along with their effects on CCAAT element binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FAS), and fatty acid-binding protein 4 (FABP4) mRNA transcriptions. FAS and FABP4 protein expression levels after exposure to CS-IVa were also tested. The results showed that DLL-Ex and CS-IVa have potent inhibitory activity on adipocyte differentiation. Therefore, DLL and CS-IVa may be developed as a functional food material to treat obesity. |
format | Online Article Text |
id | pubmed-6164478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61644782018-10-10 Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds Yin, Jun Seo, Chang-Seob Hwang, In Hyeok Lee, Min Won Song, Kwang Hoon Nutrients Article Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In the present work, we studyed the molecular mechanism for the inhibitory effect of DLL-Ex or Chikusetsusaponin IVa (CS-IVa), as isolated from Dolichos lablab L. (DLL) seeds extract, on adipocyte differentiation. We evaluated the effect of DLL-Ex, an anti-obesity agent, and CS-IVa, an active component of DLL-Ex, on 3T3-L1 cell differentiation via Oil red O assay and Q-PCR, along with their effects on CCAAT element binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FAS), and fatty acid-binding protein 4 (FABP4) mRNA transcriptions. FAS and FABP4 protein expression levels after exposure to CS-IVa were also tested. The results showed that DLL-Ex and CS-IVa have potent inhibitory activity on adipocyte differentiation. Therefore, DLL and CS-IVa may be developed as a functional food material to treat obesity. MDPI 2018-09-03 /pmc/articles/PMC6164478/ /pubmed/30177649 http://dx.doi.org/10.3390/nu10091221 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yin, Jun Seo, Chang-Seob Hwang, In Hyeok Lee, Min Won Song, Kwang Hoon Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds |
title | Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds |
title_full | Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds |
title_fullStr | Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds |
title_full_unstemmed | Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds |
title_short | Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds |
title_sort | anti-obesity activities of chikusetsusaponin iva and dolichos lablab l. seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164478/ https://www.ncbi.nlm.nih.gov/pubmed/30177649 http://dx.doi.org/10.3390/nu10091221 |
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