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Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms
Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164559/ https://www.ncbi.nlm.nih.gov/pubmed/29933583 http://dx.doi.org/10.3390/microorganisms6030056 |
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author | Peebo, Karl Neubauer, Peter |
author_facet | Peebo, Karl Neubauer, Peter |
author_sort | Peebo, Karl |
collection | PubMed |
description | Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate of the culture is advantageous. Continuous culture methods were developed to grow cells in a constant environment and have been used for decades to study basic microbial physiology in a controlled and reproducible manner. Our review summarizes the uses of continuous cultures in cell physiology studies and process development, with a focus on recombinant protein-producing microorganisms. |
format | Online Article Text |
id | pubmed-6164559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61645592018-10-10 Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms Peebo, Karl Neubauer, Peter Microorganisms Review Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate of the culture is advantageous. Continuous culture methods were developed to grow cells in a constant environment and have been used for decades to study basic microbial physiology in a controlled and reproducible manner. Our review summarizes the uses of continuous cultures in cell physiology studies and process development, with a focus on recombinant protein-producing microorganisms. MDPI 2018-06-21 /pmc/articles/PMC6164559/ /pubmed/29933583 http://dx.doi.org/10.3390/microorganisms6030056 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Peebo, Karl Neubauer, Peter Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms |
title | Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms |
title_full | Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms |
title_fullStr | Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms |
title_full_unstemmed | Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms |
title_short | Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms |
title_sort | application of continuous culture methods to recombinant protein production in microorganisms |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164559/ https://www.ncbi.nlm.nih.gov/pubmed/29933583 http://dx.doi.org/10.3390/microorganisms6030056 |
work_keys_str_mv | AT peebokarl applicationofcontinuousculturemethodstorecombinantproteinproductioninmicroorganisms AT neubauerpeter applicationofcontinuousculturemethodstorecombinantproteinproductioninmicroorganisms |