Cargando…

Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1

Ultraviolet absorbing chemicals (UV filters) are widely used in personal care products for protecting human skin and hair from damage by UV radiation. Although these substances are released into the environment during production and consumption processes, little is known about their genotoxicity eff...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Wen-Qian, Duan, Hai-Xin, Pei, Zhou-Tao, Xu, Rou-Rou, Qin, Ze-Tian, Zhu, Guang-Can, Sun, Li-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164588/
https://www.ncbi.nlm.nih.gov/pubmed/30200545
http://dx.doi.org/10.3390/ijerph15091907
_version_ 1783359636066271232
author Wang, Wen-Qian
Duan, Hai-Xin
Pei, Zhou-Tao
Xu, Rou-Rou
Qin, Ze-Tian
Zhu, Guang-Can
Sun, Li-Wei
author_facet Wang, Wen-Qian
Duan, Hai-Xin
Pei, Zhou-Tao
Xu, Rou-Rou
Qin, Ze-Tian
Zhu, Guang-Can
Sun, Li-Wei
author_sort Wang, Wen-Qian
collection PubMed
description Ultraviolet absorbing chemicals (UV filters) are widely used in personal care products for protecting human skin and hair from damage by UV radiation. Although these substances are released into the environment during production and consumption processes, little is known about their genotoxicity effects. Our previous studies have shown that benzophenone-type UV filters exhibited acute toxicity on three species of aquatic organisms. Mutagenesis by benzophenone (BP) and benzophenone-1(BP-1) was tested in the present study by the Salmonella typhimurium/reverse mutation assay (Ames assay). All the positive reverse mutations occurred in the absence of the S9 liver extract system for both chemicals. From BP, positive mutation effects on the TA102 strain at doses of 0.05 μg/plate and 0.5 μg/plate were detected. From BP-1, positive mutation effects on the TA97 strain at doses of 0.05 μg/plate and 0.5 μg/plate, and on the TA100 strain at a dose of 0.5 μg/plate, were detected. A mixture of BP and BP-1 exhibited mutagenicity on the TA97 and TA100 strains. For the TA97 strain, the positive mutation results were detected at 10% and 50% of the mixture. For the TA100 strain, the results were detected when the mixture was at 5% and 10%. In the mixture at 5%, the concentrations of BP and BP-1 were 3.5 μg/plate and 14 μg/plate, respectively. In the 10% mixture, the doses of BP and BP-1 were 7 μg/plate and 28 μg/plate, respectively. In the 50% mixture, the doses of BP and BP-1 were 35 μg/plate and 140 μg/plate, respectively. The mixture test results suggested that there was antagonism in mutagenicity between BP and BP-1.
format Online
Article
Text
id pubmed-6164588
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-61645882018-10-12 Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1 Wang, Wen-Qian Duan, Hai-Xin Pei, Zhou-Tao Xu, Rou-Rou Qin, Ze-Tian Zhu, Guang-Can Sun, Li-Wei Int J Environ Res Public Health Article Ultraviolet absorbing chemicals (UV filters) are widely used in personal care products for protecting human skin and hair from damage by UV radiation. Although these substances are released into the environment during production and consumption processes, little is known about their genotoxicity effects. Our previous studies have shown that benzophenone-type UV filters exhibited acute toxicity on three species of aquatic organisms. Mutagenesis by benzophenone (BP) and benzophenone-1(BP-1) was tested in the present study by the Salmonella typhimurium/reverse mutation assay (Ames assay). All the positive reverse mutations occurred in the absence of the S9 liver extract system for both chemicals. From BP, positive mutation effects on the TA102 strain at doses of 0.05 μg/plate and 0.5 μg/plate were detected. From BP-1, positive mutation effects on the TA97 strain at doses of 0.05 μg/plate and 0.5 μg/plate, and on the TA100 strain at a dose of 0.5 μg/plate, were detected. A mixture of BP and BP-1 exhibited mutagenicity on the TA97 and TA100 strains. For the TA97 strain, the positive mutation results were detected at 10% and 50% of the mixture. For the TA100 strain, the results were detected when the mixture was at 5% and 10%. In the mixture at 5%, the concentrations of BP and BP-1 were 3.5 μg/plate and 14 μg/plate, respectively. In the 10% mixture, the doses of BP and BP-1 were 7 μg/plate and 28 μg/plate, respectively. In the 50% mixture, the doses of BP and BP-1 were 35 μg/plate and 140 μg/plate, respectively. The mixture test results suggested that there was antagonism in mutagenicity between BP and BP-1. MDPI 2018-09-02 2018-09 /pmc/articles/PMC6164588/ /pubmed/30200545 http://dx.doi.org/10.3390/ijerph15091907 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Wen-Qian
Duan, Hai-Xin
Pei, Zhou-Tao
Xu, Rou-Rou
Qin, Ze-Tian
Zhu, Guang-Can
Sun, Li-Wei
Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1
title Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1
title_full Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1
title_fullStr Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1
title_full_unstemmed Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1
title_short Evaluation by the Ames Assay of the Mutagenicity of UV Filters Using Benzophenone and Benzophenone-1
title_sort evaluation by the ames assay of the mutagenicity of uv filters using benzophenone and benzophenone-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164588/
https://www.ncbi.nlm.nih.gov/pubmed/30200545
http://dx.doi.org/10.3390/ijerph15091907
work_keys_str_mv AT wangwenqian evaluationbytheamesassayofthemutagenicityofuvfiltersusingbenzophenoneandbenzophenone1
AT duanhaixin evaluationbytheamesassayofthemutagenicityofuvfiltersusingbenzophenoneandbenzophenone1
AT peizhoutao evaluationbytheamesassayofthemutagenicityofuvfiltersusingbenzophenoneandbenzophenone1
AT xurourou evaluationbytheamesassayofthemutagenicityofuvfiltersusingbenzophenoneandbenzophenone1
AT qinzetian evaluationbytheamesassayofthemutagenicityofuvfiltersusingbenzophenoneandbenzophenone1
AT zhuguangcan evaluationbytheamesassayofthemutagenicityofuvfiltersusingbenzophenoneandbenzophenone1
AT sunliwei evaluationbytheamesassayofthemutagenicityofuvfiltersusingbenzophenoneandbenzophenone1