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A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure

Background: Bone grafts are used in approximately one half of all musculoskeletal surgeries. Autograft bone is the historic gold standard but is limited in supply and its harvest imparts significant morbidity to the patient. Alternative sources of bone graft include allografts, synthetics and, less...

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Autores principales: Bracey, Daniel N., Seyler, Thorsten M., Jinnah, Alexander H., Lively, Mark O., Willey, Jeffrey S., Smith, Thomas L., Van Dyke, Mark E., Whitlock, Patrick W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164666/
https://www.ncbi.nlm.nih.gov/pubmed/30002336
http://dx.doi.org/10.3390/jfb9030045
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author Bracey, Daniel N.
Seyler, Thorsten M.
Jinnah, Alexander H.
Lively, Mark O.
Willey, Jeffrey S.
Smith, Thomas L.
Van Dyke, Mark E.
Whitlock, Patrick W.
author_facet Bracey, Daniel N.
Seyler, Thorsten M.
Jinnah, Alexander H.
Lively, Mark O.
Willey, Jeffrey S.
Smith, Thomas L.
Van Dyke, Mark E.
Whitlock, Patrick W.
author_sort Bracey, Daniel N.
collection PubMed
description Background: Bone grafts are used in approximately one half of all musculoskeletal surgeries. Autograft bone is the historic gold standard but is limited in supply and its harvest imparts significant morbidity to the patient. Alternative sources of bone graft include allografts, synthetics and, less commonly, xenografts which are taken from animal species. Xenografts are available in unlimited supply from healthy animal donors with controlled biology, avoiding the risk of human disease transmission, and may satisfy current demand for bone graft products. Methods: In the current study, cancellous bone was harvested from porcine femurs and subjected to a novel decellularization protocol to derive a bone scaffold. Results: The scaffold was devoid of donor cellular material on histology and DNA sampling (p < 0.01). Microarchitectural properties important for osteoconductive potential were preserved after decellularization as shown by high resolution imaging modalities. Proteomics data demonstrated similar profiles when comparing the porcine bone scaffold against commercially available human demineralized bone matrix approved for clinical use. Conclusion: We are unaware of any porcine-derived bone graft products currently used in orthopaedic surgery practice. Results from the current study suggest that porcine-derived bone scaffolds warrant further consideration to serve as a potential bone graft substitute.
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spelling pubmed-61646662018-10-12 A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure Bracey, Daniel N. Seyler, Thorsten M. Jinnah, Alexander H. Lively, Mark O. Willey, Jeffrey S. Smith, Thomas L. Van Dyke, Mark E. Whitlock, Patrick W. J Funct Biomater Article Background: Bone grafts are used in approximately one half of all musculoskeletal surgeries. Autograft bone is the historic gold standard but is limited in supply and its harvest imparts significant morbidity to the patient. Alternative sources of bone graft include allografts, synthetics and, less commonly, xenografts which are taken from animal species. Xenografts are available in unlimited supply from healthy animal donors with controlled biology, avoiding the risk of human disease transmission, and may satisfy current demand for bone graft products. Methods: In the current study, cancellous bone was harvested from porcine femurs and subjected to a novel decellularization protocol to derive a bone scaffold. Results: The scaffold was devoid of donor cellular material on histology and DNA sampling (p < 0.01). Microarchitectural properties important for osteoconductive potential were preserved after decellularization as shown by high resolution imaging modalities. Proteomics data demonstrated similar profiles when comparing the porcine bone scaffold against commercially available human demineralized bone matrix approved for clinical use. Conclusion: We are unaware of any porcine-derived bone graft products currently used in orthopaedic surgery practice. Results from the current study suggest that porcine-derived bone scaffolds warrant further consideration to serve as a potential bone graft substitute. MDPI 2018-07-12 /pmc/articles/PMC6164666/ /pubmed/30002336 http://dx.doi.org/10.3390/jfb9030045 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bracey, Daniel N.
Seyler, Thorsten M.
Jinnah, Alexander H.
Lively, Mark O.
Willey, Jeffrey S.
Smith, Thomas L.
Van Dyke, Mark E.
Whitlock, Patrick W.
A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure
title A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure
title_full A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure
title_fullStr A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure
title_full_unstemmed A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure
title_short A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure
title_sort decellularized porcine xenograft-derived bone scaffold for clinical use as a bone graft substitute: a critical evaluation of processing and structure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164666/
https://www.ncbi.nlm.nih.gov/pubmed/30002336
http://dx.doi.org/10.3390/jfb9030045
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