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Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine

Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscop...

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Autores principales: Sui, Tan, Dluhoš, Jiří, Li, Tao, Zeng, Kaiyang, Cernescu, Adrian, Landini, Gabriel, Korsunsky, Alexander M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164774/
https://www.ncbi.nlm.nih.gov/pubmed/30134596
http://dx.doi.org/10.3390/ma11091493
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author Sui, Tan
Dluhoš, Jiří
Li, Tao
Zeng, Kaiyang
Cernescu, Adrian
Landini, Gabriel
Korsunsky, Alexander M.
author_facet Sui, Tan
Dluhoš, Jiří
Li, Tao
Zeng, Kaiyang
Cernescu, Adrian
Landini, Gabriel
Korsunsky, Alexander M.
author_sort Sui, Tan
collection PubMed
description Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscopy (s-SNOM) mapping. The brighter appearance of PTD in 3D SEM-Backscattered-Electron (BSE) imaging mode and the corresponding higher grey value indicate a greater mineral concentration in PTD (~160) compared to ITD (~152). However, the 3D FIB-SEM-EDS reconstruction and high resolution, quantitative 2D map of the Ca/P ratio (~1.8) fail to distinguish between PTD and ITD. This has been further confirmed using nanoscale 2D AFM map, which clearly visualised biopolymers and hydroxyapatite (HAp) crystallites with larger mean crystallite size in ITD (32 ± 8 nm) than that in PTD (22 ± 3 nm). Correlative microscopy reveals that the principal difference between PTD and ITD arises primarily from the nanoscale packing density of the crystallites bonded together by thin biopolymer, with moderate contribution from the chemical composition difference. The structural difference results in the mechanical properties variation that is described by the parabolic stiffness-volume fraction correlation function introduced here. The obtained results benefit a microstructure-based mechano-chemical model to simulate the chemical etching process that can occur in human dental caries and some of its treatments.
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spelling pubmed-61647742018-10-12 Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine Sui, Tan Dluhoš, Jiří Li, Tao Zeng, Kaiyang Cernescu, Adrian Landini, Gabriel Korsunsky, Alexander M. Materials (Basel) Article Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscopy (s-SNOM) mapping. The brighter appearance of PTD in 3D SEM-Backscattered-Electron (BSE) imaging mode and the corresponding higher grey value indicate a greater mineral concentration in PTD (~160) compared to ITD (~152). However, the 3D FIB-SEM-EDS reconstruction and high resolution, quantitative 2D map of the Ca/P ratio (~1.8) fail to distinguish between PTD and ITD. This has been further confirmed using nanoscale 2D AFM map, which clearly visualised biopolymers and hydroxyapatite (HAp) crystallites with larger mean crystallite size in ITD (32 ± 8 nm) than that in PTD (22 ± 3 nm). Correlative microscopy reveals that the principal difference between PTD and ITD arises primarily from the nanoscale packing density of the crystallites bonded together by thin biopolymer, with moderate contribution from the chemical composition difference. The structural difference results in the mechanical properties variation that is described by the parabolic stiffness-volume fraction correlation function introduced here. The obtained results benefit a microstructure-based mechano-chemical model to simulate the chemical etching process that can occur in human dental caries and some of its treatments. MDPI 2018-08-21 /pmc/articles/PMC6164774/ /pubmed/30134596 http://dx.doi.org/10.3390/ma11091493 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sui, Tan
Dluhoš, Jiří
Li, Tao
Zeng, Kaiyang
Cernescu, Adrian
Landini, Gabriel
Korsunsky, Alexander M.
Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine
title Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine
title_full Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine
title_fullStr Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine
title_full_unstemmed Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine
title_short Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine
title_sort structure-function correlative microscopy of peritubular and intertubular dentine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164774/
https://www.ncbi.nlm.nih.gov/pubmed/30134596
http://dx.doi.org/10.3390/ma11091493
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