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Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine
Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscop...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164774/ https://www.ncbi.nlm.nih.gov/pubmed/30134596 http://dx.doi.org/10.3390/ma11091493 |
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author | Sui, Tan Dluhoš, Jiří Li, Tao Zeng, Kaiyang Cernescu, Adrian Landini, Gabriel Korsunsky, Alexander M. |
author_facet | Sui, Tan Dluhoš, Jiří Li, Tao Zeng, Kaiyang Cernescu, Adrian Landini, Gabriel Korsunsky, Alexander M. |
author_sort | Sui, Tan |
collection | PubMed |
description | Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscopy (s-SNOM) mapping. The brighter appearance of PTD in 3D SEM-Backscattered-Electron (BSE) imaging mode and the corresponding higher grey value indicate a greater mineral concentration in PTD (~160) compared to ITD (~152). However, the 3D FIB-SEM-EDS reconstruction and high resolution, quantitative 2D map of the Ca/P ratio (~1.8) fail to distinguish between PTD and ITD. This has been further confirmed using nanoscale 2D AFM map, which clearly visualised biopolymers and hydroxyapatite (HAp) crystallites with larger mean crystallite size in ITD (32 ± 8 nm) than that in PTD (22 ± 3 nm). Correlative microscopy reveals that the principal difference between PTD and ITD arises primarily from the nanoscale packing density of the crystallites bonded together by thin biopolymer, with moderate contribution from the chemical composition difference. The structural difference results in the mechanical properties variation that is described by the parabolic stiffness-volume fraction correlation function introduced here. The obtained results benefit a microstructure-based mechano-chemical model to simulate the chemical etching process that can occur in human dental caries and some of its treatments. |
format | Online Article Text |
id | pubmed-6164774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61647742018-10-12 Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine Sui, Tan Dluhoš, Jiří Li, Tao Zeng, Kaiyang Cernescu, Adrian Landini, Gabriel Korsunsky, Alexander M. Materials (Basel) Article Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscopy (s-SNOM) mapping. The brighter appearance of PTD in 3D SEM-Backscattered-Electron (BSE) imaging mode and the corresponding higher grey value indicate a greater mineral concentration in PTD (~160) compared to ITD (~152). However, the 3D FIB-SEM-EDS reconstruction and high resolution, quantitative 2D map of the Ca/P ratio (~1.8) fail to distinguish between PTD and ITD. This has been further confirmed using nanoscale 2D AFM map, which clearly visualised biopolymers and hydroxyapatite (HAp) crystallites with larger mean crystallite size in ITD (32 ± 8 nm) than that in PTD (22 ± 3 nm). Correlative microscopy reveals that the principal difference between PTD and ITD arises primarily from the nanoscale packing density of the crystallites bonded together by thin biopolymer, with moderate contribution from the chemical composition difference. The structural difference results in the mechanical properties variation that is described by the parabolic stiffness-volume fraction correlation function introduced here. The obtained results benefit a microstructure-based mechano-chemical model to simulate the chemical etching process that can occur in human dental caries and some of its treatments. MDPI 2018-08-21 /pmc/articles/PMC6164774/ /pubmed/30134596 http://dx.doi.org/10.3390/ma11091493 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sui, Tan Dluhoš, Jiří Li, Tao Zeng, Kaiyang Cernescu, Adrian Landini, Gabriel Korsunsky, Alexander M. Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine |
title | Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine |
title_full | Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine |
title_fullStr | Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine |
title_full_unstemmed | Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine |
title_short | Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine |
title_sort | structure-function correlative microscopy of peritubular and intertubular dentine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6164774/ https://www.ncbi.nlm.nih.gov/pubmed/30134596 http://dx.doi.org/10.3390/ma11091493 |
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