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A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering

Since most of the body’s extracellular matrix (ECM) is composed of collagen and elastin, we believe the choice of these materials is key for the future and promise of tissue engineering. Once it is known how elastin content of ECM guides cellular behavior (in 2D or 3D), one will be able to harness t...

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Autores principales: Wang, Xinmei, Ali, Mir S., Lacerda, Carla M. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165002/
https://www.ncbi.nlm.nih.gov/pubmed/30154331
http://dx.doi.org/10.3390/bioengineering5030069
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author Wang, Xinmei
Ali, Mir S.
Lacerda, Carla M. R.
author_facet Wang, Xinmei
Ali, Mir S.
Lacerda, Carla M. R.
author_sort Wang, Xinmei
collection PubMed
description Since most of the body’s extracellular matrix (ECM) is composed of collagen and elastin, we believe the choice of these materials is key for the future and promise of tissue engineering. Once it is known how elastin content of ECM guides cellular behavior (in 2D or 3D), one will be able to harness the power of collagen-elastin microenvironments to design and engineer stimuli-responsive tissues. Moreover, the implementation of such matrices to promote endothelial-mesenchymal transition of primary endothelial cells constitutes a powerful tool to engineer 3D tissues. Here, we design a 3D collagen-elastin scaffold to mimic the native ECM of heart valves, by providing the strength of collagen layers, as well as elasticity. Valve interstitial cells (VICs) were encapsulated in the collagen-elastin hydrogels and valve endothelial cells (VECs) cultured onto the surface to create an in vitro 3D VEC-VIC co-culture. Over a seven-day period, VICs had stable expression levels of integrin β1 and F-actin and continuously proliferated, while cell morphology changed to more elongated. VECs maintained endothelial phenotype up to day five, as indicated by low expression of F-actin and integrin β1, while transformed VECs accounted for less than 7% of the total VECs in culture. On day seven, over 20% VECs were transformed to mesenchymal phenotype, indicated by increased actin filaments and higher expression of integrin β1. These findings demonstrate that our 3D collagen-elastin scaffolds provided a novel tool to study cell-cell or cell-matrix interactions in vitro, promoting advances in the current knowledge of valvular endothelial cell mesenchymal transition.
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spelling pubmed-61650022018-10-11 A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering Wang, Xinmei Ali, Mir S. Lacerda, Carla M. R. Bioengineering (Basel) Article Since most of the body’s extracellular matrix (ECM) is composed of collagen and elastin, we believe the choice of these materials is key for the future and promise of tissue engineering. Once it is known how elastin content of ECM guides cellular behavior (in 2D or 3D), one will be able to harness the power of collagen-elastin microenvironments to design and engineer stimuli-responsive tissues. Moreover, the implementation of such matrices to promote endothelial-mesenchymal transition of primary endothelial cells constitutes a powerful tool to engineer 3D tissues. Here, we design a 3D collagen-elastin scaffold to mimic the native ECM of heart valves, by providing the strength of collagen layers, as well as elasticity. Valve interstitial cells (VICs) were encapsulated in the collagen-elastin hydrogels and valve endothelial cells (VECs) cultured onto the surface to create an in vitro 3D VEC-VIC co-culture. Over a seven-day period, VICs had stable expression levels of integrin β1 and F-actin and continuously proliferated, while cell morphology changed to more elongated. VECs maintained endothelial phenotype up to day five, as indicated by low expression of F-actin and integrin β1, while transformed VECs accounted for less than 7% of the total VECs in culture. On day seven, over 20% VECs were transformed to mesenchymal phenotype, indicated by increased actin filaments and higher expression of integrin β1. These findings demonstrate that our 3D collagen-elastin scaffolds provided a novel tool to study cell-cell or cell-matrix interactions in vitro, promoting advances in the current knowledge of valvular endothelial cell mesenchymal transition. MDPI 2018-08-28 /pmc/articles/PMC6165002/ /pubmed/30154331 http://dx.doi.org/10.3390/bioengineering5030069 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xinmei
Ali, Mir S.
Lacerda, Carla M. R.
A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering
title A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering
title_full A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering
title_fullStr A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering
title_full_unstemmed A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering
title_short A Three-Dimensional Collagen-Elastin Scaffold for Heart Valve Tissue Engineering
title_sort three-dimensional collagen-elastin scaffold for heart valve tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165002/
https://www.ncbi.nlm.nih.gov/pubmed/30154331
http://dx.doi.org/10.3390/bioengineering5030069
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