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New 19-Residue Peptaibols from Trichoderma Clade Viride

Trichoderma koningiopsis and T. gamsii belong to clade Viride of Trichoderma, the largest and most diverse group of this genus. They produce a wide range of bioactive secondary metabolites, including peptaibols with antibacterial, antifungal, and antiviral properties. The unusual amino acid residues...

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Autores principales: Marik, Tamás, Tyagi, Chetna, Racić, Gordana, Rakk, Dávid, Szekeres, András, Vágvölgyi, Csaba, Kredics, László
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165201/
https://www.ncbi.nlm.nih.gov/pubmed/30103563
http://dx.doi.org/10.3390/microorganisms6030085
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author Marik, Tamás
Tyagi, Chetna
Racić, Gordana
Rakk, Dávid
Szekeres, András
Vágvölgyi, Csaba
Kredics, László
author_facet Marik, Tamás
Tyagi, Chetna
Racić, Gordana
Rakk, Dávid
Szekeres, András
Vágvölgyi, Csaba
Kredics, László
author_sort Marik, Tamás
collection PubMed
description Trichoderma koningiopsis and T. gamsii belong to clade Viride of Trichoderma, the largest and most diverse group of this genus. They produce a wide range of bioactive secondary metabolites, including peptaibols with antibacterial, antifungal, and antiviral properties. The unusual amino acid residues of peptaibols, i.e., α-aminoisobutyric acid (Aib), isovaline (Iva), and the C-terminal 1,2-amino alcohol make them unique among peptides. In this study, the peptaibiomes of T. koningiopsis and T. gamsii were investigated by HPLC-ESI-MS. The examined strains appeared to produce 19-residue peptaibols, most of which are unknown from literature, but their amino acid sequences are similar to those of trikoningins, tricholongins, trichostrigocins, trichorzianins, and trichorzins. A new group of peptaibols detected in T. koningiopsis are described here under the name “Koningiopsin”. Trikoningin KA V, the closest peptaibol compound to the peptaibols produced by these two strains, was selected for structural investigation by short MD simulation, which revealed that many residues show high preference for left handed helix formation. The bioactivity of the peptaibol mixtures produced by T. koningiopsis and T. gamsii was tested on agar plates against bacteria, yeasts, and filamentous fungi. The results revealed characteristic differences in bioactivities towards the different groups of target microorganisms, which can be explained with the differences in their cell wall structures.
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spelling pubmed-61652012018-10-10 New 19-Residue Peptaibols from Trichoderma Clade Viride Marik, Tamás Tyagi, Chetna Racić, Gordana Rakk, Dávid Szekeres, András Vágvölgyi, Csaba Kredics, László Microorganisms Article Trichoderma koningiopsis and T. gamsii belong to clade Viride of Trichoderma, the largest and most diverse group of this genus. They produce a wide range of bioactive secondary metabolites, including peptaibols with antibacterial, antifungal, and antiviral properties. The unusual amino acid residues of peptaibols, i.e., α-aminoisobutyric acid (Aib), isovaline (Iva), and the C-terminal 1,2-amino alcohol make them unique among peptides. In this study, the peptaibiomes of T. koningiopsis and T. gamsii were investigated by HPLC-ESI-MS. The examined strains appeared to produce 19-residue peptaibols, most of which are unknown from literature, but their amino acid sequences are similar to those of trikoningins, tricholongins, trichostrigocins, trichorzianins, and trichorzins. A new group of peptaibols detected in T. koningiopsis are described here under the name “Koningiopsin”. Trikoningin KA V, the closest peptaibol compound to the peptaibols produced by these two strains, was selected for structural investigation by short MD simulation, which revealed that many residues show high preference for left handed helix formation. The bioactivity of the peptaibol mixtures produced by T. koningiopsis and T. gamsii was tested on agar plates against bacteria, yeasts, and filamentous fungi. The results revealed characteristic differences in bioactivities towards the different groups of target microorganisms, which can be explained with the differences in their cell wall structures. MDPI 2018-08-12 /pmc/articles/PMC6165201/ /pubmed/30103563 http://dx.doi.org/10.3390/microorganisms6030085 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marik, Tamás
Tyagi, Chetna
Racić, Gordana
Rakk, Dávid
Szekeres, András
Vágvölgyi, Csaba
Kredics, László
New 19-Residue Peptaibols from Trichoderma Clade Viride
title New 19-Residue Peptaibols from Trichoderma Clade Viride
title_full New 19-Residue Peptaibols from Trichoderma Clade Viride
title_fullStr New 19-Residue Peptaibols from Trichoderma Clade Viride
title_full_unstemmed New 19-Residue Peptaibols from Trichoderma Clade Viride
title_short New 19-Residue Peptaibols from Trichoderma Clade Viride
title_sort new 19-residue peptaibols from trichoderma clade viride
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165201/
https://www.ncbi.nlm.nih.gov/pubmed/30103563
http://dx.doi.org/10.3390/microorganisms6030085
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