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Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2

Peritubular cells are part of the wall of seminiferous tubules in the human testis and their contractile abilities are important for sperm transport. In addition, they have immunological roles. A proteomic analysis of isolated human testicular peritubular cells (HTPCs) revealed expression of the tra...

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Autores principales: Eubler, Katja, Herrmann, Carola, Tiefenbacher, Astrid, Köhn, Frank-Michael, Schwarzer, J. Ullrich, Kunz, Lars, Mayerhofer, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165404/
https://www.ncbi.nlm.nih.gov/pubmed/30235802
http://dx.doi.org/10.3390/ijms19092829
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author Eubler, Katja
Herrmann, Carola
Tiefenbacher, Astrid
Köhn, Frank-Michael
Schwarzer, J. Ullrich
Kunz, Lars
Mayerhofer, Artur
author_facet Eubler, Katja
Herrmann, Carola
Tiefenbacher, Astrid
Köhn, Frank-Michael
Schwarzer, J. Ullrich
Kunz, Lars
Mayerhofer, Artur
author_sort Eubler, Katja
collection PubMed
description Peritubular cells are part of the wall of seminiferous tubules in the human testis and their contractile abilities are important for sperm transport. In addition, they have immunological roles. A proteomic analysis of isolated human testicular peritubular cells (HTPCs) revealed expression of the transient receptor potential channel subfamily V member 2 (TRPV2). This cation channel is linked to mechano-sensation and to immunological processes and inflammation in other organs. We verified expression of TRPV2 in peritubular cells in human sections by immunohistochemistry. It was also found in other testicular cells, including Sertoli cells and interstitial cells. In cultured HTPCs, application of cannabidiol (CBD), a known TRPV2 agonist, acutely induced a transient increase in intracellular Ca(2+) levels. These Ca(2+) transients could be blocked both by ruthenium red, an unspecific Ca(2+) channel blocker, and tranilast (TRA), an antagonist of TRPV2, and were also abolished when extracellular Ca(2+) was removed. Taken together this indicates functional TRPV2 channels in peritubular cells. When applied for 24 to 48 h, CBD induced expression of proinflammatory factors. In particular, mRNA and secreted protein levels of the proinflammatory chemokine interleukin-8 (IL-8/CXCL8) were elevated. Via its known roles as a major mediator of the inflammatory response and as an angiogenic factor, this chemokine may play a role in testicular physiology and pathology.
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spelling pubmed-61654042018-10-10 Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2 Eubler, Katja Herrmann, Carola Tiefenbacher, Astrid Köhn, Frank-Michael Schwarzer, J. Ullrich Kunz, Lars Mayerhofer, Artur Int J Mol Sci Article Peritubular cells are part of the wall of seminiferous tubules in the human testis and their contractile abilities are important for sperm transport. In addition, they have immunological roles. A proteomic analysis of isolated human testicular peritubular cells (HTPCs) revealed expression of the transient receptor potential channel subfamily V member 2 (TRPV2). This cation channel is linked to mechano-sensation and to immunological processes and inflammation in other organs. We verified expression of TRPV2 in peritubular cells in human sections by immunohistochemistry. It was also found in other testicular cells, including Sertoli cells and interstitial cells. In cultured HTPCs, application of cannabidiol (CBD), a known TRPV2 agonist, acutely induced a transient increase in intracellular Ca(2+) levels. These Ca(2+) transients could be blocked both by ruthenium red, an unspecific Ca(2+) channel blocker, and tranilast (TRA), an antagonist of TRPV2, and were also abolished when extracellular Ca(2+) was removed. Taken together this indicates functional TRPV2 channels in peritubular cells. When applied for 24 to 48 h, CBD induced expression of proinflammatory factors. In particular, mRNA and secreted protein levels of the proinflammatory chemokine interleukin-8 (IL-8/CXCL8) were elevated. Via its known roles as a major mediator of the inflammatory response and as an angiogenic factor, this chemokine may play a role in testicular physiology and pathology. MDPI 2018-09-19 /pmc/articles/PMC6165404/ /pubmed/30235802 http://dx.doi.org/10.3390/ijms19092829 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Eubler, Katja
Herrmann, Carola
Tiefenbacher, Astrid
Köhn, Frank-Michael
Schwarzer, J. Ullrich
Kunz, Lars
Mayerhofer, Artur
Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
title Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
title_full Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
title_fullStr Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
title_full_unstemmed Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
title_short Ca(2+) Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
title_sort ca(2+) signaling and il-8 secretion in human testicular peritubular cells involve the cation channel trpv2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165404/
https://www.ncbi.nlm.nih.gov/pubmed/30235802
http://dx.doi.org/10.3390/ijms19092829
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