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Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection
Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. Blockage by Wolbachia in some scenarios may be affected by the susceptibility of wMel to cyclical heat stress during mo...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165456/ https://www.ncbi.nlm.nih.gov/pubmed/29966368 http://dx.doi.org/10.3390/insects9030078 |
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author | Ross, Perran A. Hoffmann, Ary A. |
author_facet | Ross, Perran A. Hoffmann, Ary A. |
author_sort | Ross, Perran A. |
collection | PubMed |
description | Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. Blockage by Wolbachia in some scenarios may be affected by the susceptibility of wMel to cyclical heat stress during mosquito larval development. We therefore evaluated the potential to generate a heat-resistant strain of wMel in Ae. aegypti through artificial laboratory selection and through exposure to field temperatures across multiple generations. To generate an artificially selected strain, wMel-infected females reared under cyclical heat stress were crossed to wMel-infected males reared at 26 °C. The low proportion of larvae that hatched founded the next generation, and this process was repeated for eight generations. The wMel heat-selected strain (wMel-HS) was similar to wMel (unselected) in its ability to induce cytoplasmic incompatibility and restore compatibility when larvae were reared under cyclical heat stress, but wMel-HS adults exhibited reduced Wolbachia densities at 26 °C. To investigate the effects of field exposure, we compared the response of wMel-infected Ae. aegypti collected from Cairns, Australia where the infection has been established for seven years, to a wMel-infected population maintained in the laboratory for approximately 60 generations. Field and laboratory strains of wMel did not differ in their response to cyclical heat stress or in their phenotypic effects at 26 °C. The capacity for the wMel infection in Ae. aegypti to adapt to high temperatures therefore appears limited, and alternative strains may need to be considered for deployment in environments where high temperatures are regularly experienced in mosquito breeding sites. |
format | Online Article Text |
id | pubmed-6165456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61654562018-10-10 Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection Ross, Perran A. Hoffmann, Ary A. Insects Article Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. Blockage by Wolbachia in some scenarios may be affected by the susceptibility of wMel to cyclical heat stress during mosquito larval development. We therefore evaluated the potential to generate a heat-resistant strain of wMel in Ae. aegypti through artificial laboratory selection and through exposure to field temperatures across multiple generations. To generate an artificially selected strain, wMel-infected females reared under cyclical heat stress were crossed to wMel-infected males reared at 26 °C. The low proportion of larvae that hatched founded the next generation, and this process was repeated for eight generations. The wMel heat-selected strain (wMel-HS) was similar to wMel (unselected) in its ability to induce cytoplasmic incompatibility and restore compatibility when larvae were reared under cyclical heat stress, but wMel-HS adults exhibited reduced Wolbachia densities at 26 °C. To investigate the effects of field exposure, we compared the response of wMel-infected Ae. aegypti collected from Cairns, Australia where the infection has been established for seven years, to a wMel-infected population maintained in the laboratory for approximately 60 generations. Field and laboratory strains of wMel did not differ in their response to cyclical heat stress or in their phenotypic effects at 26 °C. The capacity for the wMel infection in Ae. aegypti to adapt to high temperatures therefore appears limited, and alternative strains may need to be considered for deployment in environments where high temperatures are regularly experienced in mosquito breeding sites. MDPI 2018-06-30 /pmc/articles/PMC6165456/ /pubmed/29966368 http://dx.doi.org/10.3390/insects9030078 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ross, Perran A. Hoffmann, Ary A. Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection |
title | Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection |
title_full | Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection |
title_fullStr | Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection |
title_full_unstemmed | Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection |
title_short | Continued Susceptibility of the wMel Wolbachia Infection in Aedes aegypti to Heat Stress Following Field Deployment and Selection |
title_sort | continued susceptibility of the wmel wolbachia infection in aedes aegypti to heat stress following field deployment and selection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165456/ https://www.ncbi.nlm.nih.gov/pubmed/29966368 http://dx.doi.org/10.3390/insects9030078 |
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